Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,013,331 T→C 16.4% V35A (GTG→GCG)  PP_5678 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,013,3310TC16.4% 162.8 / 22.3 73V35A (GTG→GCG) PP_5678hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base C (5/7);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTT  >  NC_002947/5013187‑5013478
                                                                                                                                                |                                                                                                                                                   
aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                                >  1:611945/1‑150 (MQ=255)
aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                                >  1:576573/1‑150 (MQ=255)
  ccGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGc                                                                                                                                                   <  2:1302334/145‑1 (MQ=255)
  ccGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGc                                                                                                                                                   >  1:1302334/1‑145 (MQ=255)
  ccGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAgc                                                                                                                                              >  2:1290443/1‑150 (MQ=255)
   cGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAgcg                                                                                                                                             >  1:1164249/1‑150 (MQ=255)
      gCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAggatgg                                                                                                                                          <  2:611945/150‑6 (MQ=255)
       cAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggc                                                                                                                                         <  2:440552/150‑1 (MQ=255)
        aGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCgcggcg                                                                                                                                        >  1:819985/1‑150 (MQ=255)
         gTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGt                                                                                                                                       <  2:672510/150‑1 (MQ=255)
          tGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                      <  2:91705/150‑1 (MQ=255)
            caacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgct                                                                                                                                    <  2:1382665/150‑3 (MQ=255)
             aacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                      >  2:451115/1‑147 (MQ=255)
             aacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                      <  1:451115/147‑1 (MQ=255)
                   ccTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                             <  1:989120/150‑1 (MQ=255)
                   ccTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                             <  1:792638/150‑1 (MQ=255)
                    cTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGc                                                                                                                            >  2:1052034/1‑150 (MQ=255)
                        gACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgc                                                                                                                        >  1:810283/1‑150 (MQ=255)
                           ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                                  <  1:121569/137‑5 (MQ=255)
                           ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                                  >  2:121569/1‑133 (MQ=255)
                              ggggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                             <  2:523656/139‑1 (MQ=255)
                              ggggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                             >  1:523656/1‑139 (MQ=255)
                              ggggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCaaa                                                                                                                  >  1:1029926/1‑150 (MQ=255)
                                      ccAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                          >  2:539210/1‑150 (MQ=255)
                                        aaTGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAAATACCAGCTCGCTGTTGGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGc                                                                                                        <  1:247797/150‑1 (MQ=255)
                                           gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                     <  1:1290443/150‑1 (MQ=255)
                                            atgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTAt                                                                                                    <  1:1090122/150‑1 (MQ=255)
                                                  aaaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAGCCCCGAAGCCTATGAACTc                                                                                              >  2:800291/1‑150 (MQ=255)
                                                  aaaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAACGCGACGTGGAAATCAGACTCGCAACAAAACCCCAAAGCCTATAAACTc                                                                                              >  2:435574/1‑150 (MQ=255)
                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCa                                                                                                                               <  1:279617/116‑1 (MQ=255)
                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCa                                                                                                                               >  2:279617/1‑116 (MQ=255)
                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                             >  2:1056409/1‑150 (MQ=255)
                                                             ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                   >  1:427070/1‑150 (MQ=255)
                                                             ccTCGCAGCCGTTGCAGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGAAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgc                                                                                   >  2:124668/1‑149 (MQ=255)
                                                               tcacAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggc                                                                                                                                         <  1:558388/91‑1 (MQ=255)
                                                               tcacAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggc                                                                                                                                         >  2:558388/4‑94 (MQ=255)
                                                               tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCAt                                                                                           <  1:641462/140‑1 (MQ=255)
                                                               tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCAt                                                                                           >  2:641462/1‑140 (MQ=255)
                                                                 gCAGCCGTTGCAGTCAGCGAAGCTAGCGACCGCTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgc                                                                               >  2:642056/1‑150 (MQ=255)
                                                                          gCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCTAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCttt                                                                      <  2:819985/150‑1 (MQ=255)
                                                                              tCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCa                                                                  <  1:1056409/150‑1 (MQ=255)
                                                                                            gACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGc                                                                                                        >  1:1349330/1‑98 (MQ=255)
                                                                                            gACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGc                                                                                                        <  2:1349330/98‑1 (MQ=255)
                                                                                            gACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATa                                                    >  1:301335/1‑150 (MQ=255)
                                                                                            gACCGTTGCGGCCAGACTTGTCCTTATGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATa                                                    >  2:511762/1‑150 (MQ=255)
                                                                                              ccGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                                  <  2:427070/150‑1 (MQ=255)
                                                                                              ccGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                                  <  2:1364081/150‑1 (MQ=255)
                                                                                                 ttGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                     >  2:576847/1‑96 (MQ=255)
                                                                                                 ttGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                     <  1:576847/96‑1 (MQ=255)
                                                                                                        cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                        >  1:257860/1‑150 (MQ=255)
                                                                                                          gACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCTAGCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTg                                      <  1:1169909/150‑1 (MQ=255)
                                                                                                          gACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTg                                      <  2:333227/150‑1 (MQ=255)
                                                                                                             ttGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTgg                                     <  2:207190/148‑1 (MQ=255)
                                                                                                             ttGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTgg                                     >  1:207190/1‑148 (MQ=255)
                                                                                                                   ttCTGATGACGGCCATCCACAGCACGCCGTGCTGCGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                             <  1:435574/150‑1 (MQ=255)
                                                                                                                   ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                             >  2:832059/1‑150 (MQ=255)
                                                                                                                   ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                             >  2:1105920/1‑150 (MQ=255)
                                                                                                                      tgatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGAt                          >  2:1023436/1‑150 (MQ=255)
                                                                                                                                  tgatttccACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGa              >  2:906291/8‑150 (MQ=255)
                                                                                                                                   gatttccACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGaa             <  1:906291/144‑1 (MQ=255)
                                                                                                                                   ccACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGaa             <  2:301335/150‑1 (MQ=255)
                                                                                                                                   ccACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGaa             <  1:1023436/150‑1 (MQ=255)
                                                                                                                                     tttccACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCttt                                                                      >  2:1285772/5‑91 (MQ=38)
                                                                                                                                     tttccACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCttt                                                                      <  1:1285772/87‑1 (MQ=38)
                                                                                                                                        gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa        <  1:1052034/150‑1 (MQ=255)
                                                                                                                                        gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa        <  1:539210/150‑1 (MQ=255)
                                                                                                                                        gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTAAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa        <  1:1105920/150‑1 (MQ=255)
                                                                                                                                        ccACGCCGTGCGGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGACTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa        <  1:124668/149‑1 (MQ=255)
                                                                                                                                          aCGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGAAAAGCTTTGAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACt      >  2:903214/1‑150 (MQ=255)
                                                                                                                                             ccGTGCTGAGCGCGGCGTGGAAATCAGGNTCGCAGCANAACCCCGAANCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAACGTGAACGCTGGGCCCAATTGATGTTCTGTGAGGAAGTTGGCTCCt   >  1:522583/1‑150 (MQ=255)
                                                                                                                                              cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAGCCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCtt  <  1:800291/150‑1 (MQ=255)
                                                                                                                                              cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATTTTCTCTGAGGAAGCTGACTCCtt  <  1:389991/150‑1 (MQ=255)
                                                                                                                                              cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCtt  >  2:389991/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTT  >  NC_002947/5013187‑5013478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑