| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,029,037 | C→T | 9.3% | intergenic (+66/‑373) | PP_4432 → / → PP_4433 | Xaa‑Pro aminopeptidase/amino acid MFS transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,029,037 | 0 | C | T | 9.3% | 136.5 / 5.5 | 54 | intergenic (+66/‑373) | PP_4432/PP_4433 | Xaa‑Pro aminopeptidase/amino acid MFS transporter |
| Reads supporting (aligned to +/- strand): ref base C (23/25); new base T (3/2); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
AGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGTAATA > NC_002947/5028889‑5029186 | agaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCt < 2:1381175/150‑1 (MQ=255)agaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCt < 1:1374681/150‑1 (MQ=255) gaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCtt > 2:1286943/1‑150 (MQ=255) tACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTaaa > 1:382878/1‑150 (MQ=255) tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAcattg > 2:704624/1‑146 (MQ=255) aTCACTGCTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGc < 2:396658/150‑1 (MQ=255) tGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc > 1:497063/1‑150 (MQ=255) aTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCt > 2:1387467/1‑150 (MQ=255) tGGGGTCGAGACGTTCTGCCCGAGGCCTCGCAAGCTTTTCGTCAAGGACTGCCGAAAGGTTTTCAAGTCGCTGGAGTTATAGTGCCACGGCTATTTTCAGCGACAATGAGCTCATATAGTAAAGCACCATCACTCCAGCCACTCCTTTGt > 2:1181908/1‑150 (MQ=255) gTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACaaaaaaa < 1:1286943/150‑1 (MQ=255) tCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGc < 2:382878/150‑1 (MQ=255) tGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCACCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCAc < 1:1181908/150‑1 (MQ=255) gCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACg < 2:498684/150‑1 (MQ=255) cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAc < 1:498106/115‑1 (MQ=255) cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAc > 2:498106/1‑115 (MQ=255) tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt > 2:902730/1‑150 (MQ=255) tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt > 2:1297666/1‑150 (MQ=255) tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt < 1:902730/150‑1 (MQ=255) ctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCt > 2:142664/1‑147 (MQ=255) ctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCt < 1:142664/147‑1 (MQ=255) tcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGaa < 1:820128/150‑1 (MQ=255) tcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGaa > 2:820128/1‑150 (MQ=255) ttttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCCGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGa > 1:743113/1‑150 (MQ=255) tttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc < 2:283040/95‑1 (MQ=255) tttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc > 1:283040/1‑95 (MQ=255) cAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa < 1:1297666/150‑1 (MQ=255) aaGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGAt < 1:1395434/150‑1 (MQ=255) cTGACGACAAGTTTTCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTAAAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTc < 2:747530/150‑1 (MQ=255) aCGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTg > 1:847525/1‑98 (MQ=255) aCGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTg < 2:847525/98‑1 (MQ=255) gAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAg > 1:935048/1‑150 (MQ=255) aaaGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAggg > 2:1051359/1‑150 (MQ=255) gCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCa < 1:598885/70‑1 (MQ=255) gCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCa > 2:598885/1‑70 (MQ=255) aaGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCTCCATCACTCTAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGa > 2:937908/1‑146 (MQ=255) aaGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCTCCATCACTCTAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGa < 1:937908/146‑1 (MQ=255) cGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCt < 2:497063/150‑1 (MQ=255) tGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCtgtg > 2:396940/1‑150 (MQ=255) tGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCtgtg > 2:436225/1‑150 (MQ=255) gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa > 1:46450/1‑117 (MQ=255) gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa < 2:46450/117‑1 (MQ=255) gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAAc > 2:1174473/1‑141 (MQ=255) gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAAc < 1:1174473/141‑1 (MQ=255) gTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCt < 1:1051359/150‑1 (MQ=255) ccACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGaa > 1:1338474/1‑150 (MQ=255) cACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGaaa < 1:396940/150‑1 (MQ=255) ggCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACtt < 2:743113/150‑1 (MQ=255) aGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGgacg > 1:539786/1‑150 (MQ=255) tgtgATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGaa > 2:631969/1‑150 (MQ=255) tgATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATa > 2:656762/1‑150 (MQ=255) tGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGaa < 1:436225/150‑1 (MQ=255) gTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGt < 2:539786/150‑1 (MQ=255) gCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGga < 2:1332077/131‑1 (MQ=255) gCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGga > 1:1332077/1‑131 (MQ=255) cTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCCAGCAGCAGCCCGGCAA‑GGGTACGAAACGCCTGAAACTTATCAAGGACGACGCGGAATAGAAGTAATa > 1:168242/1‑150 (MQ=255) | AGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGTAATA > NC_002947/5028889‑5029186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |