Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,029,042 A→G 12.9% intergenic (+71/‑368) PP_4432 → / → PP_4433 Xaa‑Pro aminopeptidase/amino acid MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,029,0420AG12.9% 130.8 / 8.7 53intergenic (+71/‑368)PP_4432/PP_4433Xaa‑Pro aminopeptidase/amino acid MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base G (4/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGTAATA  >  NC_002947/5028893‑5029186
                                                                                                                                                     |                                                                                                                                                 
tACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTaaa                                                                                                                                                   >  1:382878/1‑150 (MQ=255)
              tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAcattg                                                                                                                                     >  2:704624/1‑146 (MQ=255)
                aTCACTGCTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGc                                                                                                                                   <  2:396658/150‑1 (MQ=255)
                     tGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc                                                                                                                              >  1:497063/1‑150 (MQ=255)
                       aTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCt                                                                                                                            >  2:1387467/1‑150 (MQ=255)
                           tGGGGTCGAGACGTTCTGCCCGAGGCCTCGCAAGCTTTTCGTCAAGGACTGCCGAAAGGTTTTCAAGTCGCTGGAGTTATAGTGCCACGGCTATTTTCAGCGACAATGAGCTCATATAGTAAAGCACCATCACTCCAGCCACTCCTTTGt                                                                                                                        >  2:1181908/1‑150 (MQ=255)
                                       gTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACaaaaaaa                                                                                                            <  1:1286943/150‑1 (MQ=255)
                                         tCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGc                                                                                                          <  2:382878/150‑1 (MQ=255)
                                           tGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCACCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCAc                                                                                                        <  1:1181908/150‑1 (MQ=255)
                                            gCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACg                                                                                                       <  2:498684/150‑1 (MQ=255)
                                             cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAc                                                                                                                                         >  2:498106/1‑115 (MQ=255)
                                             cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCAc                                                                                                                                         <  1:498106/115‑1 (MQ=255)
                                                   tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt                                                                                                >  2:902730/1‑150 (MQ=255)
                                                   tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt                                                                                                >  2:1297666/1‑150 (MQ=255)
                                                   tcctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt                                                                                                <  1:902730/150‑1 (MQ=255)
                                                     ctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCt                                                                                                 >  2:142664/1‑147 (MQ=255)
                                                     ctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCt                                                                                                 <  1:142664/147‑1 (MQ=255)
                                                      tcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGaa                                                                                             <  1:820128/150‑1 (MQ=255)
                                                      tcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGaa                                                                                             >  2:820128/1‑150 (MQ=255)
                                                              ttttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCCGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGa                                                                                     >  1:743113/1‑150 (MQ=255)
                                                               tttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc                                                                                                                                           <  2:283040/95‑1 (MQ=255)
                                                               tttCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc                                                                                                                                           >  1:283040/1‑95 (MQ=255)
                                                                     cAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa                                                                              <  1:1297666/150‑1 (MQ=255)
                                                                      aaGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGAt                                                                             <  1:1395434/150‑1 (MQ=255)
                                                                           cTGACGACAAGTTTTCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTAAAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTc                                                                        <  2:747530/150‑1 (MQ=255)
                                                                              aCGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTg                                                                                                                         >  1:847525/1‑98 (MQ=255)
                                                                              aCGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTg                                                                                                                         <  2:847525/98‑1 (MQ=255)
                                                                                gAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAg                                                                  >  1:935048/1‑150 (MQ=255)
                                                                                  aaaGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAggg                                                                >  2:1051359/1‑150 (MQ=255)
                                                                                         gCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCa                                                                                                                                          <  1:598885/70‑1 (MQ=255)
                                                                                         gCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCa                                                                                                                                          >  2:598885/1‑70 (MQ=255)
                                                                                           aaGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCTCCATCACTCTAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGa                                                           >  2:937908/1‑146 (MQ=255)
                                                                                           aaGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCTCCATCACTCTAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGa                                                           <  1:937908/146‑1 (MQ=255)
                                                                                               cGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCt                                                   <  2:497063/150‑1 (MQ=255)
                                                                                                  tGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCtgtg                                                >  2:396940/1‑150 (MQ=255)
                                                                                                  tGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCtgtg                                                >  2:436225/1‑150 (MQ=255)
                                                                                                      gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa                                                                              <  2:46450/117‑1 (MQ=255)
                                                                                                      gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGa                                                                              >  1:46450/1‑117 (MQ=255)
                                                                                                      gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAAc                                                     >  2:1174473/1‑141 (MQ=255)
                                                                                                      gTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAAc                                                     <  1:1174473/141‑1 (MQ=255)
                                                                                                            gTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCt                                      <  1:1051359/150‑1 (MQ=255)
                                                                                                               ccACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGaa                                   >  1:1338474/1‑150 (MQ=255)
                                                                                                                cACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGaaa                                  <  1:396940/150‑1 (MQ=255)
                                                                                                                   ggCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACtt                               <  2:743113/150‑1 (MQ=255)
                                                                                                                             aGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGgacg                     >  1:539786/1‑150 (MQ=255)
                                                                                                                                     tgtgATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGaa             >  2:631969/1‑150 (MQ=255)
                                                                                                                                       tgATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATa           >  2:656762/1‑150 (MQ=255)
                                                                                                                                          tGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGaa        <  1:436225/150‑1 (MQ=255)
                                                                                                                                            gTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGt      <  2:539786/150‑1 (MQ=255)
                                                                                                                                              gCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGga                       <  2:1332077/131‑1 (MQ=255)
                                                                                                                                              gCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGga                       >  1:1332077/1‑131 (MQ=255)
                                                                                                                                                tttAAGGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGAtt                                                                            >  2:160112/2‑77 (MQ=255)
                                                                                                                                                tttAAGGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGAtt                                                                            <  1:160112/76‑1 (MQ=255)
                                                                                                                                                cTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCCAGCAGCAGCCCGGCAA‑GGGTACGAAACGCCTGAAACTTATCAAGGACGACGCGGAATAGAAGTAATa  >  1:168242/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAGGTTGAAAGTTGATTGTTCCC‑CAGGGAGCGAGTCAACCTGTGCCTAACGCCTGAAACTTATCAAGGACGACGCTGAATAGAAGTAATA  >  NC_002947/5028893‑5029186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑