Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,039,903 C→A 7.7% E217* (GAG→TAG)  PP_4446 ← group II intron‑encoding maturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,039,9030CA 7.7% 160.2 / 3.2 52E217* (GAG→TAG) PP_4446group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base A (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

TCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACAATAATTACATCGCCACGG  >  NC_002947/5039760‑5040051
                                                                                                                                               |                                                                                                                                                    
tCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAg                                                                                                                                                >  2:1058103/1‑150 (MQ=255)
  gCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGcc                                                                                                                                              >  1:365116/1‑150 (MQ=255)
    ttcttGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCgt                                                                                                                                            >  1:627949/1‑150 (MQ=255)
            cGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGa                                                                                                                                    <  1:1058103/150‑1 (MQ=255)
              tCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGcc                                                                                                                                              <  2:787634/138‑1 (MQ=255)
              tCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGcc                                                                                                                                              >  1:787634/1‑138 (MQ=255)
                    gCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAGGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCa                                                                                                                            <  2:245316/150‑1 (MQ=255)
                     cAGTATTTGAAACCCACCGCTTCTGTTGGTACTACCACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTGCTCGCCGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAg                                                                                                                           <  2:760876/150‑1 (MQ=255)
                     cAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAg                                                                                                                           >  1:1067682/1‑150 (MQ=255)
                     cAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAg                                                                                                                           <  2:780306/150‑1 (MQ=255)
                       gTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAggg                                                                                                                         >  2:927631/1‑150 (MQ=255)
                             gAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAAc                                                                                                                   <  1:927631/150‑1 (MQ=255)
                                   cACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCGCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGAGATTTCTCGCAGCAAAAAGACCGAACTCAATTAGCCGTTTCTTCGAGGCGGTCAGGGATAAACCGAAcc                                                                                                             >  2:311385/1‑150 (MQ=255)
                                     ccGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTCGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTg                                                                                                           <  2:820136/150‑1 (MQ=255)
                                              ttGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAAc                                                                                                              >  1:676016/1‑138 (MQ=255)
                                              ttGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAAc                                                                                                              <  2:676016/138‑1 (MQ=255)
                                                ggTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTc                                                                                                <  2:1067682/150‑1 (MQ=255)
                                                 gTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTcc                                                                                               >  2:559781/1‑150 (MQ=255)
                                                 gTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTcc                                                                                               >  1:1019916/1‑150 (MQ=255)
                                                  tactacAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCt                                                                                              <  2:892211/150‑1 (MQ=255)
                                                     tacAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCa                                                                                           >  1:1254320/1‑150 (MQ=255)
                                                        aacaGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGCACTCAATTAGc                                                                                                                                               >  2:44086/1‑95 (MQ=255)
                                                        aacaGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGc                                                                                                                                               <  1:44086/95‑1 (MQ=255)
                                                        aacaGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACt                                                                                        >  2:1098183/1‑150 (MQ=255)
                                                          caGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTg                                                                                             <  1:1233182/143‑1 (MQ=255)
                                                          caGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTg                                                                                             >  2:1233182/1‑143 (MQ=255)
                                                                gTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGa                                                                                >  2:1104186/1‑150 (MQ=255)
                                                                             aGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCt                                                                   <  1:559781/150‑1 (MQ=255)
                                                                                     gTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTc                                                           >  1:327248/1‑150 (MQ=255)
                                                                                     gTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTc                                                           <  2:327248/150‑1 (MQ=255)
                                                                                        tcCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAAc                                                                                                                   >  1:623090/1‑91 (MQ=255)
                                                                                        tcCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAAc                                                                                                                   <  2:623090/91‑1 (MQ=255)
                                                                                        tcCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTAAATTAGCCGTGTCTTCGAGGCGTTCAgg                                                                                                                          >  1:559774/1‑84 (MQ=255)
                                                                                        tcCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTAAATTAGCCGTGTCTTCGAGGCGTTCAgg                                                                                                                          <  2:559774/84‑1 (MQ=255)
                                                                                         ccGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTAAATTa                                                                                                                                                 <  1:345928/60‑1 (MQ=255)
                                                                                         ccGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTAAATTa                                                                                                                                                 >  2:345928/1‑60 (MQ=255)
                                                                                                  cTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACcca                                              <  2:365116/150‑1 (MQ=255)
                                                                                                         tCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTc                                                           >  1:1294881/1‑130 (MQ=255)
                                                                                                         tCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTc                                                           <  2:1294881/130‑1 (MQ=255)
                                                                                                         tCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCt                                       >  1:280823/1‑150 (MQ=255)
                                                                                                               ccTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTgtcgtc                                 <  1:194149/150‑1 (MQ=255)
                                                                                                                 tGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACccac                                             <  2:478440/136‑1 (MQ=255)
                                                                                                                 tGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACccac                                             >  1:478440/1‑136 (MQ=255)
                                                                                                                 tGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCg                               >  1:486505/1‑150 (MQ=255)
                                                                                                                 tGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGCAGTACCTGGCCTACAACTGCACCAGAAACTGCTGAGACTGCACTCTCCTCCTGAAACCTACCACGCTGTCGTCCg                               >  1:957776/1‑150 (MQ=255)
                                                                                                                          tcGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGca                      <  2:486505/150‑1 (MQ=255)
                                                                                                                                 aaaaCGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACaataat               >  2:839462/1‑150 (MQ=255)
                                                                                                                                 aaaaCGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAAATGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACaataat               <  1:1098183/150‑1 (MQ=255)
                                                                                                                                  aaaCGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCCTAACGCACAATAAtt              <  2:280823/150‑1 (MQ=255)
                                                                                                                                   aaCGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACAATAATTa             >  2:187379/1‑150 (MQ=255)
                                                                                                                                           aaCTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACAATAATTACATCGCCa     <  1:257052/150‑1 (MQ=255)
                                                                                                                                              tCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACAATAATTACATCGCCACgg  <  2:1254320/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TCGCTTCTTGACCGTCAGTCGCAGTATTTGAAACCCACCGCTTCTGTTGGTACTACAACAGTGCGTGAAGCCCAGGAAGTCAAACGTCTCCGGTTTACCTAACCCTCGCTTCCTGCGATTTCTCGCAGCAAAACGACCGAACTCAATTAGCCGTGTCTTCGAGGCGTTCAGGGATAAACCGAACCTGGCCAACCGTTCCTGCAACTGCACCAGAAACTGCTGAGCCTGCACTTTCGTCCTGAAACCCACCACGCTGTCGTCCGCATAACGCACAATAATTACATCGCCACGG  >  NC_002947/5039760‑5040051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑