| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,080,879 | C→G | 8.5% | P236P (CCG→CCC) | lysC ← | aspartokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,080,879 | 0 | C | G | 8.5% | 139.6 / 4.7 | 47 | P236P (CCG→CCC) | lysC | aspartokinase |
| Reads supporting (aligned to +/- strand): ref base C (23/20); new base G (2/2); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGCGACGGGCCTGTG > NC_002947/5080737‑5081027 | tCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGaccct < 2:718426‑M2/150‑6 (MQ=255) gggCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACcctcc < 1:937300/150‑1 (MQ=255) ccACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCCGGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCtt > 1:811942/1‑133 (MQ=255) ccACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCtt < 2:811942/133‑1 (MQ=255) ggCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTAc < 2:859948/150‑1 (MQ=255) aTGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCAGGCCCAGCGGAACGTTGTACTTGCCGGCGAAc > 1:315240/1‑150 (MQ=255) tCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTAATCAATGGTGTTGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCa < 1:883881/150‑1 (MQ=255) cAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCAc > 2:1056422/1‑150 (MQ=255) ttCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGAt > 1:851598/1‑150 (MQ=255) ttCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGAt > 1:285298/1‑150 (MQ=255) atcaCGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTg > 1:102951/1‑150 (MQ=255) tcaCGGTTGAAGGCGATACCGGAAATGATCGGCTGTTGCAGGGATCCCTCTTCATCACTGGTAATGAGGGTACTCGGACCGTCCTCTACGCTGTGTAGCACGCCCGGCGGAATGCTGGAATTCCCAACGCACTCCACCGAACGAATCTgc > 2:586527/1‑150 (MQ=255) tcaCGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTgc < 2:285298/150‑1 (MQ=255) tcaCGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTATTAATAAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTCTACTTGACGGCGAACTCCACCGAACAGATCTgc > 2:630922/1‑150 (MQ=255) gAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCtt < 2:851598/150‑1 (MQ=255) ggCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGa < 1:1056422/150‑1 (MQ=255) ggCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGT‑CCGGGtaccctcattaccattgatgaaga < 2:1349812‑M2/89‑25 (MQ=255) ggCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGT‑CCGGGtaccctcattaccattgatgaaga > 1:1349812‑M2/1‑65 (MQ=255) gCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTCCGGGTaccctcattaccat < 1:673068‑M2/79‑15 (MQ=255) gCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTCCGGGTaccctcattaccat > 2:673068‑M2/1‑65 (MQ=255) cGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTCTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCaa < 1:815354/147‑1 (MQ=255) cGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTCTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCaa > 2:815354/1‑147 (MQ=255) tCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCAttt > 2:1390522/1‑150 (MQ=255) tCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCAttt > 1:243321/1‑150 (MQ=255) gCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCa < 2:102951/150‑1 (MQ=255) cgatctCCATGGATTCCTCTTCATCAATGGTAATGGCGCAATCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCa < 1:74236/145‑1 (MQ=255) cTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAg < 1:718426/150‑1 (MQ=255) gTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTg < 1:1134427/137‑1 (MQ=255) gTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTg > 2:1134427/1‑137 (MQ=255) tGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCtt < 1:1390522/150‑1 (MQ=255) tCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAg > 2:1237732/1‑150 (MQ=255) ctctTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGaa > 1:572798/1‑147 (MQ=255) ctctTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGaa < 2:572798/147‑1 (MQ=255) ctctTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGt > 1:1224844/1‑150 (MQ=255) tctTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCa < 2:196461/133‑1 (MQ=255) tctTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCa > 1:196461/1‑133 (MQ=255) tcaATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCCACCGGAGCGATAAGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGAtcttc > 2:1416676/1‑150 (MQ=255) tcaATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGAtcttc > 1:950625/1‑150 (MQ=255) tAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTc < 1:1075745/118‑1 (MQ=255) tAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTc < 1:1075927/118‑1 (MQ=255) tAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTc > 2:1075927/1‑118 (MQ=255) tAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTc > 2:1075745/1‑118 (MQ=255) tAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCCTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGc > 2:310667/1‑150 (MQ=255) aTGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGCGa > 2:957397/1‑150 (MQ=255) gAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGCGACg < 1:630922/150‑1 (MQ=255) aGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTgc < 1:397400/84‑1 (MQ=255) aGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTgc > 2:397400/1‑84 (MQ=255) ccGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGCGACGGGCCtgtg > 2:753740/1‑150 (MQ=255) | TCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATGGATTCCTCTTCATCAATGGTAATGAGGGTACCCGGACCCTCCTTGAAGCTGTGCAGCACGCGCAGCGGAACGTTGTACTTGCCGGCGAACTCCACCGAACGGATCTGCAGCACCTTGGAACCAAGGCTGGCCATTTCCAGCATCTCTTCGAAGGTGATCTTCTCCAGGCGACGGGCCTGTG > NC_002947/5080737‑5081027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |