| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,083,466 | G→C | 8.2% | A283G (GCC→GGC) | alaS ← | alanine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,083,466 | 0 | G | C | 8.2% | 190.0 / 6.8 | 61 | A283G (GCC→GGC) | alaS | alanine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base C (3/2); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GATCTGGTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACGCTCCAGGCCCATGCC > NC_002947/5083318‑5083608 | gATCTGGTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGc < 1:559672/150‑1 (MQ=255) cTGGTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGAc < 2:74800/150‑1 (MQ=255) ggTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCa > 2:1261666/1‑150 (MQ=255) gTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCAc > 2:427650/1‑150 (MQ=255) agaagCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCa < 1:54427/150‑1 (MQ=255) agCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCg > 2:1308081/1‑150 (MQ=255) gCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGa > 1:595972/1‑150 (MQ=255) cTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTTGTCGGCGACCACTTTCAGCGaa < 1:1261666/150‑1 (MQ=255) cTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTg < 2:1103451/150‑1 (MQ=255) cTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTg > 1:917310/1‑150 (MQ=255) gcCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAAGAAGAGAGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTc > 1:74017/1‑150 (MQ=255) ccAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGGGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCAt > 2:455290/1‑150 (MQ=255) gccgcgcttgCCGTCACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCAtt > 2:1162164/8‑150 (MQ=255) cAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCAtt > 1:999909/1‑150 (MQ=255) aGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGTACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATtg < 1:878941/150‑1 (MQ=255) cTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATtgct < 1:592489/150‑1 (MQ=255) ttgttgCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGAAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATtgctg < 1:852620/150‑1 (MQ=255) ttgCCGTCACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCaa < 1:1162164/150‑1 (MQ=255) cGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGa < 1:135705/150‑1 (MQ=255) tGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATg > 2:1017102/1‑150 (MQ=255) gCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAAAGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCtt > 2:1261323/1‑150 (MQ=255) gcgcGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGc < 1:1355818/98‑1 (MQ=255) gcgcGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGc > 2:1355818/1‑98 (MQ=255) gcggcgAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGc < 1:1308081/150‑1 (MQ=255) gAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGACAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGcag > 1:271749/1‑150 (MQ=255) tgCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGc > 1:476521/1‑85 (MQ=255) tgCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGc < 2:476521/85‑1 (MQ=255) tgCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCcagca > 2:520767/1‑150 (MQ=255) gcggcgCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCcagcag < 1:292259/150‑1 (MQ=255) gcggcgCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGGCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCcagcag < 2:670024/150‑1 (MQ=255) gcgcAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGtt < 2:1237840/150‑1 (MQ=255) gcagcaCGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCt > 2:245563/1‑150 (MQ=255) cagcaCGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGACAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTg < 2:271749/150‑1 (MQ=255) caCGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGaa < 1:491929/150‑1 (MQ=255) aCGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGTACGAACTGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAAc < 1:25146/150‑1 (MQ=255) cGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACa < 1:301626/150‑1 (MQ=255) ccTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCg < 1:520767/150‑1 (MQ=255) ttCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGTGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTa > 1:176791/1‑150 (MQ=255) ttCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGACCTCATTGCTGCCACCGCTGCACTTCTCTGCGGCAGCCATCAGGTTCTGGAACAGGTCGATCTCTTa < 2:234768/150‑1 (MQ=255) gTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTg > 2:700439/1‑78 (MQ=255) gTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTg < 1:700439/78‑1 (MQ=255) gTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGt > 2:799803/1‑83 (MQ=255) gTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGt < 1:799803/83‑1 (MQ=255) ttGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTc < 2:595972/150‑1 (MQ=255) gACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTTGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGaa < 2:1121790/150‑1 (MQ=255) gcaCGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAATTCGAATGCACGt < 1:1017102/150‑1 (MQ=255) ccGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTTGGGAACAGGTCGATCTCGTAGTTCGAATGCACGtgctgc > 1:154582/1‑150 (MQ=255) ggCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCAc > 1:584197/1‑150 (MQ=255) gCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACg > 2:1102052/1‑150 (MQ=255) tCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTGGAGCTCGTATTTCGAATGCACGTGCTGTAGGACAgcgt > 2:691205/1‑149 (MQ=255) tCAGGAAGCCGCACGAACGGATGTGGTCGGAGCACACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGgcgc > 1:691447/1‑150 (MQ=255) gCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACg > 2:664731/1‑150 (MQ=255) gCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACGCTc > 1:527756/1‑150 (MQ=255) aaCGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTa < 2:526900/106‑1 (MQ=255) aaCGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTa > 1:526900/1‑106 (MQ=255) tgaaagtgGTCGCCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACGCTCCAGGccc > 2:1205865/6‑150 (MQ=255) aaagtgGTCGCCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACg < 1:53888/124‑1 (MQ=255) aaagtgGTCGCCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACg < 1:53454/124‑1 (MQ=255) aaagtgGTCGCCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACg > 2:53454/4‑127 (MQ=255) aaagtgGTCGCCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACg > 2:53888/4‑127 (MQ=255) gtgGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACGCTCCAGGCCCATGcc < 1:115339/150‑1 (MQ=255) | GATCTGGTAGAAGAAGCTGCCCTTGGCGCCCAGCTTGTTGCCGTGACGGCAAGCGCGGCGAATGATGCGGCGCAGCACGTAGCCACGGCCTTCGTTGGACGGCAGCACGCCGTCGGCAATCAGGAAGCCGCACGAACGGATGTGGTCGGCGACCACTTTCAGCGAAGCCTGGCCGTCATTGCTGCAACCGATGGCCTTGGCTGCGGCAGCCAGCAGGTTCTGGAACAGGTCGATCTCGTAGTTCGAATGCACGTGCTGCAGCACGGCGCTGACACGCTCCAGGCCCATGCC > NC_002947/5083318‑5083608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |