Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,169,782 T→A 5.9% L451Q (CTG→CAG)  PP_4547 → glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,169,7820TA 5.9% 210.5 / 4.0 68L451Q (CTG→CAG) PP_4547glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (34/30);  new base A (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

GGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGTGGAT  >  NC_002947/5169635‑5169930
                                                                                                                                                   |                                                                                                                                                     
ggatGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTga                                                                                                                                                     >  2:574459/4‑149 (MQ=255)
    gACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCa                                                                                                                                                 <  2:398930/150‑1 (MQ=255)
       cGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAgtc                                                                                                                                              <  2:1296002/150‑3 (MQ=255)
       cGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGc                                                                                                                                              >  1:377407/1‑150 (MQ=255)
           cTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCCGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGa                                                                                                                                          >  2:15494/1‑150 (MQ=255)
                    cTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTaaa                                                                                                                                 >  1:263352/1‑150 (MQ=255)
                     tGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCt                                                                                                                                                       <  2:1359678/127‑1 (MQ=255)
                     tGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCt                                                                                                                                                       >  1:1359678/1‑127 (MQ=255)
                          cATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACaa                                                                                                                          <  2:931720/150‑1 (MQ=255)
                          cATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCCGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACaa                                                                                                                          <  1:15494/150‑1 (MQ=255)
                            ttCCGATTGGGCCCGTGAAGGATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAgg                                                                                                                        >  1:440118/1‑150 (MQ=255)
                            ttCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAgg                                                                                                                        <  2:624747/150‑1 (MQ=255)
                             tacGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCGGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGa                                                                                                                       >  1:289615/3‑150 (MQ=255)
                                 aTTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAacac                                                                                                                   <  2:263352/150‑1 (MQ=255)
                                        ccGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGaa                                                                                                            >  1:237745/1‑150 (MQ=255)
                                           tGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAAc                                                                                                           >  1:10135/1‑148 (MQ=255)
                                           tGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAAc                                                                                                           <  2:10135/148‑1 (MQ=255)
                                           tGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACgc                                                                                                         >  2:878975/1‑150 (MQ=255)
                                                 aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAt                                                                                                                                <  2:122000/122‑1 (MQ=255)
                                                 aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAt                                                                                                                                >  1:122000/1‑122 (MQ=255)
                                                 aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCAtt                                                                                                   <  2:237745/150‑1 (MQ=255)
                                                   tGGGCTGAGACTGGCTGGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGa                                                                                                 <  2:658757/150‑1 (MQ=255)
                                                     ggCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGaac                                                                                               >  1:942088/1‑150 (MQ=255)
                                                        gAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGaacaac                                                                                            <  1:1039064/150‑1 (MQ=255)
                                                                 cTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGc                                                                                                                                              >  2:1110437/1‑92 (MQ=255)
                                                                 cTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGc                                                                                                                                              <  1:1110437/92‑1 (MQ=255)
                                                                       gtgtACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAgt                                                                                                                                               >  2:884188/1‑84 (MQ=25)
                                                                       gtgtACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAgt                                                                                                                                               <  1:884188/85‑2 (MQ=37)
                                                                       gtgtACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAgcgc                                                                             >  2:1337845/1‑150 (MQ=255)
                                                                        tgtACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAgcgcg                                                                            >  2:1178279/1‑150 (MQ=255)
                                                                             cTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGcc                                                                                                                                             <  2:529483/81‑1 (MQ=255)
                                                                             cTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGcc                                                                                                                                             >  1:529483/1‑81 (MQ=255)
                                                                              tGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGt                                                                      <  2:440118/150‑1 (MQ=255)
                                                                              tGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCAGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAAACTACG‑ACATCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGt                                                                      >  1:1156085/1‑150 (MQ=255)
                                                                               gAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTc                                                                     <  1:1178279/150‑1 (MQ=255)
                                                                                   gTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCctac                                                                 <  1:416991/150‑1 (MQ=255)
                                                                                       aGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAAc                                                                                                           >  2:884152/1‑104 (MQ=255)
                                                                                       aGCGCGCCGAGTACCGCCAGTTCATGGCAGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAAc                                                                                                           <  1:884152/104‑1 (MQ=255)
                                                                                         cgcgcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAgc                                                           <  2:377407/150‑1 (MQ=255)
                                                                                            gcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGcc                                                        >  1:1197863/1‑150 (MQ=255)
                                                                                            gcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGcc                                                        >  1:1197156/1‑150 (MQ=255)
                                                                                                     cGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAAc                                               <  1:981082/150‑1 (MQ=255)
                                                                                                     cGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAAc                                               >  2:876118/1‑150 (MQ=255)
                                                                                                     cGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAAc                                               >  1:606344/1‑150 (MQ=255)
                                                                                                      gCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACg                                              >  2:887143/1‑150 (MQ=255)
                                                                                                      gCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACg                                              >  1:1075286/1‑150 (MQ=255)
                                                                                                       ccAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGa                                             >  1:782670/1‑150 (MQ=255)
                                                                                                       ccAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCAACAGGCCTGAACCCCTAAAT‑AAGGAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGa                                             <  1:878975/150‑1 (MQ=255)
                                                                                                        cAGCTCATGGCCGAAGGCAGCGAACAGCACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACCCCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGAc                                            <  2:145404/150‑1 (MQ=255)
                                                                                                          gTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCAGGCGCAGCGCGCGTCGTCCTACTACAGCGCCACCCTCCACGACca                                          <  1:1176574/150‑1 (MQ=255)
                                                                                                                ggCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGc                                    <  2:782670/150‑1 (MQ=255)
                                                                                                                ggCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGc                                    <  2:956658/150‑1 (MQ=255)
                                                                                                                   cGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTa                                 <  2:289615/150‑1 (MQ=255)
                                                                                                                           gCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCa                                                 <  2:505230/126‑1 (MQ=255)
                                                                                                                           gCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCa                                                 >  1:505230/1‑126 (MQ=255)
                                                                                                                            cGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACAGCGTACCCGCAACt                        >  2:369229/1‑150 (MQ=255)
                                                                                                                             gAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTc                       >  2:1326321/1‑150 (MQ=255)
                                                                                                                                 aGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCAACCTCAACGACCCCCCTGCGTACCCGCAACTCAAgg                   <  2:1156085/150‑1 (MQ=255)
                                                                                                                                   gccTGGTGCAGGTACCAGCGCCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGc                 >  1:1353054/3‑150 (MQ=255)
                                                                                                                                            tGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAg        <  1:887143/150‑1 (MQ=255)
                                                                                                                                            tGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAg        <  1:1326321/150‑1 (MQ=255)
                                                                                                                                            tGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAg        >  1:64770/1‑150 (MQ=255)
                                                                                                                                              gTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGACGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGt      >  1:1017649/1‑150 (MQ=255)
                                                                                                                                                aCCTGCAACAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGTgg    >  2:1421429/1‑150 (MQ=255)
                                                                                                                                                 ccTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAAc                                               >  2:345530/1‑106 (MQ=255)
                                                                                                                                                 ccTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAAc                                               <  1:345530/106‑1 (MQ=255)
                                                                                                                                                  cTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAg        >  1:746008/1‑144 (MQ=255)
                                                                                                                                                  cTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAg        <  2:746008/144‑1 (MQ=255)
                                                                                                                                                  cTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGTGGAt  >  1:1254869/1‑150 (MQ=255)
                                                                                                                                                  cTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGTGGAt  >  2:505954/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAAT‑AACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAAGGGCACGGTGCAGGTGGAT  >  NC_002947/5169635‑5169930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑