Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,232,105 C→G 13.5% P213P (CCG→CCC)  PP_4610 ← PepSY‑associated TM helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,232,1050CG13.5% 128.0 / 15.0 52P213P (CCG→CCC) PP_4610PepSY‑associated TM helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base G (2/5);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

CCGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGA  >  NC_002947/5231957‑5232250
                                                                                                                                                    |                                                                                                                                                 
ccGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgccg                                                                                                                                                  <  2:1273306/150‑1 (MQ=255)
ccGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgccg                                                                                                                                                  >  1:298740/1‑150 (MQ=255)
   gCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCa                                                                                                                                               <  1:743094/150‑1 (MQ=255)
       cAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCat                                                                                                                                           <  2:109331/150‑2 (MQ=255)
       cAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCat                                                                                                                                           <  2:850144/150‑2 (MQ=255)
        aGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatg                                                                                                                                          <  2:268304/150‑3 (MQ=255)
        aGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatg                                                                                                                                          <  1:417537/150‑3 (MQ=255)
          cTCCTGCCCCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCAt                                                                                                                                        <  2:564871/150‑1 (MQ=255)
          cTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCAt                                                                                                                                        >  1:1031698/1‑150 (MQ=255)
           tCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATg                                                                                                                                       >  2:99143/1‑150 (MQ=255)
           tCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATg                                                                                                                                       >  1:366878/1‑150 (MQ=255)
             cTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTa                                                                                                                                     <  2:366878/150‑1 (MQ=255)
                   cTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGaa                                                                                                                               >  2:368953/1‑150 (MQ=255)
                   cTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGaa                                                                                                                               >  2:1125293/1‑150 (MQ=255)
                     tGGCGCACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACa                                                                                                                             <  1:922670/150‑1 (MQ=255)
                                cTCGGCAGTGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCa                                                                                                                                         >  2:97779/1‑127 (MQ=255)
                                cTCGGCAGTGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCa                                                                                                                                         <  1:97779/127‑1 (MQ=255)
                                          cTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGa                                                                                                        <  1:157213/150‑1 (MQ=255)
                                               aGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTCCTCCGCTGTGGCAGTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatcatc                                                                                                   <  1:678472/150‑1 (MQ=255)
                                                gcggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTGCATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                  <  1:982963/150‑1 (MQ=255)
                                                   gcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGt                                                                                               <  2:1031698/150‑1 (MQ=255)
                                                         ccACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgcc                                                                                                                                                   >  1:16416/1‑92 (MQ=255)
                                                         ccACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgcc                                                                                                                                                   <  2:16416/92‑1 (MQ=255)
                                                            ccTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGgca                                                                                      <  2:298740/150‑1 (MQ=255)
                                                            ccTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGgca                                                                                      >  1:860251/1‑150 (MQ=255)
                                                                ggCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcac                                                                                  <  1:368953/150‑1 (MQ=255)
                                                                 gccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCCTTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                  <  1:1185249/133‑1 (MQ=255)
                                                                 gccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                  >  2:1185249/1‑133 (MQ=255)
                                                                  ccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgccg                                                                                                                                                  <  2:6897/84‑1 (MQ=255)
                                                                  ccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgccg                                                                                                                                                  >  1:6897/1‑84 (MQ=255)
                                                                            cgcATCGTTGCGGCGCAACAGGTCGTTGAAGTCCTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCAGCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGAt                                                                      <  2:499636/150‑1 (MQ=255)
                                                                             gcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATg                                                                     <  2:1275125/150‑1 (MQ=255)
                                                                             gcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATg                                                                     >  2:1091392/1‑150 (MQ=255)
                                                                             gcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATCGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATg                                                                     <  2:166818/150‑1 (MQ=255)
                                                                                  tttGCGGCCAAACAGGTCGTTGAAGTACTTTTCGGTGTCATTTCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCATGTGAAACGGCTGCACCAGCACTCCGATGGCAtt                                                                <  2:1003498/149‑1 (MQ=255)
                                                                                      cGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGc                                                            <  1:99143/150‑1 (MQ=255)
                                                                                          cAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGa                                                                                                        >  2:719938/1‑102 (MQ=255)
                                                                                          cAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGa                                                                                                        <  1:719938/102‑1 (MQ=255)
                                                                                               aGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTccagc                                                   >  1:787732/1‑150 (MQ=255)
                                                                                                ggTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGt                                                                                               <  2:1327136/105‑1 (MQ=255)
                                                                                                ggTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcaccagcac                                                                            <  2:63216/124‑1 (MQ=255)
                                                                                                ggTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcaccagcac                                                                            >  1:63216/1‑124 (MQ=255)
                                                                                                ggTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGAACGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGt                                                                                               >  1:1327136/1‑105 (MQ=255)
                                                                                                  tCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTccagccag                                                >  1:75361/1‑150 (MQ=255)
                                                                                                     ttGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTccagccag                                                >  1:279603/1‑147 (MQ=255)
                                                                                                     ttGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTccagccag                                                <  2:279603/147‑1 (MQ=255)
                                                                                                               tGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCt                                   <  1:1256627/150‑1 (MQ=255)
                                                                                                                      gtCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAgg                            >  1:1371420/1‑150 (MQ=255)
                                                                                                                                 aGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTga                 <  1:1125293/150‑1 (MQ=255)
                                                                                                                                  gCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaa                <  2:860251/150‑1 (MQ=255)
                                                                                                                                   tacatggtgatgCCGGCGGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaag               >  2:1315415/8‑150 (MQ=255)
                                                                                                                                    tGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaaga              >  2:148763/1‑150 (MQ=255)
                                                                                                                                      atggtgatgCCGGCGGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAAc            >  1:298639/5‑150 (MQ=255)
                                                                                                                                        cATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGaaa                                                                                           <  2:1054981/69‑1 (MQ=255)
                                                                                                                                        cATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGaaa                                                                                           >  1:1054981/1‑69 (MQ=255)
                                                                                                                                        cATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTc          >  1:214966/1‑150 (MQ=255)
                                                                                                                                        cATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTc          >  2:962992/1‑150 (MQ=255)
                                                                                                                                           gatgCCGGCGGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCtt       <  2:298639/150‑1 (MQ=255)
                                                                                                                                                ccgccgGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGa  <  1:1091392/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAAGA  >  NC_002947/5231957‑5232250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑