| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,264,344 | C→A | 12.0% | intergenic (‑50/+34) | zinU ← / ← PP_4640 | P‑loop guanosine triphosphatase‑dependent zinc chaperone/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,264,344 | 0 | C | A | 12.0% | 136.1 / 7.6 | 50 | intergenic (‑50/+34) | zinU/PP_4640 | P‑loop guanosine triphosphatase‑dependent zinc chaperone/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base A (4/2); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 | |||||||||||
CGGCGAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACA > NC_002947/5264195‑5264492 | cGGCGAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTAc < 1:491205/150‑1 (MQ=255) gCGAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTAccc > 2:1119417/1‑150 (MQ=255) cTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCt < 2:663066/150‑1 (MQ=255) ttCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTc > 2:439461/1‑150 (MQ=255) ccATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAg < 1:765167/150‑1 (MQ=255) ccATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGAGGCCACAg > 1:60290/1‑150 (MQ=255) cATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCGTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAgg > 2:1174534/1‑150 (MQ=255) gAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCg < 1:439461/150‑1 (MQ=255) cATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAg < 2:2863/150‑1 (MQ=255) aTGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAAGACTCCTGAATCTGAAAATGACTCTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAgg < 2:640365/150‑1 (MQ=255) tACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTc > 2:1267096/1‑150 (MQ=255) cAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCAc > 1:140796/1‑150 (MQ=255) ggtggtCTTGCCGGCACCGAGGAAGCCTGTAAATCTACACACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTg < 2:1181912/150‑1 (MQ=255) tCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTGCTGAATCTGCAAGTGACTGTGGGAGCC‑GGTTACCCAGGCCCCCACAGGTTTACACGAACCGCAGGCCGAGCCGCACCTGCGccc > 2:48433/1‑150 (MQ=255) ttGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCgg > 2:618881/1‑150 (MQ=255) ggCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTg > 2:796842/1‑150 (MQ=255) aCCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCa < 1:1267096/150‑1 (MQ=255) aGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACg < 2:1330853/150‑1 (MQ=255) gCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCCACGAGGTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGt > 1:375038/1‑150 (MQ=255) gAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTcc < 2:688630/150‑1 (MQ=255) gACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCcaca > 2:964784/1‑84 (MQ=25) gACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCcaca < 1:964784/84‑1 (MQ=25) ttACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTc < 1:796842/150‑1 (MQ=255) ttACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGAACTCGCGTCCGTAACGTGCTTCCTGACGttt > 1:714704/1‑149 (MQ=255) cGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGttagga < 1:1119417/150‑5 (MQ=255) gggAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCgg > 2:1335337/1‑150 (MQ=255) gCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GTGTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTTCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAAttc > 2:200530/1‑148 (MQ=255) cacagaaaaTACGCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAg < 1:48433/145‑1 (MQ=255) cACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAg < 1:823991/150‑1 (MQ=255) cACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAg < 1:1174534/150‑1 (MQ=255) cGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAg < 2:610177/85‑1 (MQ=255) cGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAg > 1:610177/1‑85 (MQ=255) aaaaTACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAg > 1:1380525/1‑56 (MQ=255) aaaaTACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAg < 2:1380525/56‑1 (MQ=255) aTACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCAc < 2:60290/150‑1 (MQ=255) tactacGGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCAcc > 1:1058984/1‑150 (MQ=255) gcTCCTGAATCGGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCg < 2:454302/149‑1 (MQ=255) gAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGt > 2:869762/1‑150 (MQ=255) tCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCCCATCACCGCCTTGTCCg > 2:227101/1‑150 (MQ=255) gAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGAt < 1:618881/150‑1 (MQ=255) tgtaaaccTGTGGGAGCCTGGGTAACC‑GGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATg > 2:842008/8‑150 (MQ=38) cTGTGGGAGCCTGGGTAACC‑GGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTgg > 1:700613/1‑64 (MQ=18) cTGTGGGAGCCTGGGTAACC‑GGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTgg < 2:700613/64‑1 (MQ=18) cTGTGGGAGCCTGGGTAACC‑GGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTc > 1:821490/1‑150 (MQ=255) tgtgGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCt > 1:96869/1‑150 (MQ=255) ggAGCC‑GGTTACCCAGGCTCCCACAGGCATGTACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCAt > 1:37787/1‑150 (MQ=255) aGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCAtgt < 2:140796/150‑1 (MQ=255) cc‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATgtgt > 1:472887/1‑150 (MQ=255) gTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGgaa > 2:1323828/1‑95 (MQ=255) gTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGgaa < 1:1323828/95‑1 (MQ=255) aCCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACa < 2:472887/150‑1 (MQ=255) aCCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACa > 1:872026/1‑150 (MQ=255) | CGGCGAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTACACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACA > NC_002947/5264195‑5264492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |