| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,268,999 | T→A | 12.3% | intergenic (+19/+17) | PP_4643 → / ← radA | xanthine/uracil permease family protein/DNA repair enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,268,999 | 0 | T | A | 12.3% | 133.6 / 9.0 | 50 | intergenic (+19/+17) | PP_4643/radA | xanthine/uracil permease family protein/DNA repair enzyme |
| Reads supporting (aligned to +/- strand): ref base T (25/18); new base A (2/4); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGCTTCCTTC > NC_002947/5268858‑5269148 | cGTGATGGGTACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCAAgctcgct < 1:528624/150‑1 (MQ=255) gTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTgctcgctc < 1:877098/150‑1 (MQ=255) gTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTgctcgctc < 2:1099614/150‑1 (MQ=255) gTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTgctcgctc < 1:134482/150‑1 (MQ=255) gTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCAAgctcgctc < 2:1133124/150‑1 (MQ=255) gggACTACTTCAAGGATGGCAAGCTGTTCGATGCCGGGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGGCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCAc > 2:70973/1‑150 (MQ=255) gggACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCAc > 2:466633/1‑150 (MQ=255) actactTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCAAGCTCGCTCCt > 1:844192/1‑143 (MQ=255) actactTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCAAGCTCGCTCCt < 2:844192/143‑1 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGGCGAGCACTGACGGGTTGGAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCg > 1:487821/1‑150 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCTTGCACACTATTCg > 2:357400/1‑150 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTa > 2:1383644/1‑146 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTa < 1:1383644/146‑1 (MQ=255) cAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGaa < 2:1177697/150‑1 (MQ=255) aaGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAAc > 2:1173309/1‑150 (MQ=255) ggATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACaa > 1:1245918/1‑150 (MQ=255) cAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATc < 1:1173309/150‑1 (MQ=255) cTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAgc < 1:228969/150‑1 (MQ=255) tGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATc < 2:1098818/145‑1 (MQ=255) tGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATc > 1:1098818/1‑145 (MQ=255) ttCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCaa < 2:600232/124‑2 (MQ=255) ttCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCaa > 1:600232/1‑123 (MQ=255) ttCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGcc > 1:1128971/1‑150 (MQ=255) ccGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGaa > 2:980114/1‑127 (MQ=255) ccGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGaa < 1:980114/127‑1 (MQ=255) gggTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGGCGTTGCCGAGCAGAGACGGGTTGCAGGAGCGAGATTGCTCGCTACTGCGCGCTTTTCGAACAATGCATCCAGCGCCTGTTCCAGCCg > 1:672312/1‑150 (MQ=255) ggTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg < 2:1245918/150‑1 (MQ=255) ggTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTGCCAGCCgg < 2:1128971/150‑1 (MQ=255) cGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTaa > 1:1072949/1‑141 (MQ=255) cGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTaa < 2:1072949/141‑1 (MQ=255) cGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCa > 1:634127/1‑150 (MQ=255) ggAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCaa > 1:456708/1‑150 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg < 1:633798/120‑1 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg > 2:633798/1‑120 (MQ=255) ggCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCATCGCCTGTTCCAGCCGAGTAACGACAATCACCTGCAACCCCGCAGGCGcttct > 1:1406792/1‑150 (MQ=255) ggCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTaa > 2:1361304/1‑118 (MQ=255) ggCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTaa < 1:1361304/118‑1 (MQ=255) cAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCttt > 1:196719/1‑150 (MQ=255) aaCGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGgcgc > 2:831033/1‑150 (MQ=255) gcgcACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCtt > 1:584770/1‑150 (MQ=255) gcACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTgg < 2:634127/150‑1 (MQ=255) ggCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCAc < 2:1342983/150‑1 (MQ=255) cGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACg > 1:370088/1‑150 (MQ=255) tGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAg > 2:244485/1‑150 (MQ=255) gggTTGCAGGAGCGAGCCTCCTCCCTTCTGCCCGCTATTCCAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACTGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTAGGCACGATGGCACGCTTGAAGCCGt < 2:487821/150‑1 (MQ=255) aGGAGCGAGCAAGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg > 2:856848/1‑64 (MQ=37) aGGAGCGAGCAAGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg < 1:856848/64‑1 (MQ=37) agcgagcTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGc > 1:426715/1‑150 (MQ=255) gcgagcTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg < 1:943906/60‑1 (MQ=255) gcgagcTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg > 2:943906/1‑60 (MQ=255) ttGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGACTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGcttccttc > 1:1357183/1‑150 (MQ=255) | CGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGCTTCCTTC > NC_002947/5268858‑5269148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |