| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,351,390 | A→C | 9.3% | intergenic (+21/+55) | PP_4706 → / ← PP_4707 | hypothetical protein/OsmY‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,351,390 | 0 | A | C | 9.3% | 155.4 / 6.9 | 61 | intergenic (+21/+55) | PP_4706/PP_4707 | hypothetical protein/OsmY‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (27/28); new base C (4/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
ATGATGCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAAC > NC_002947/5351242‑5351537 | atgatgCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCAc > 1:1189515/1‑150 (MQ=255) gatgCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACgg < 2:224232/150‑1 (MQ=255) tgCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGc < 1:1193951/150‑1 (MQ=255) cgTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAggg < 1:193769/150‑1 (MQ=255) tGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTc < 2:241225/150‑1 (MQ=255) cgcgCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGAt < 1:1090587/150‑1 (MQ=255) gcgcATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATg > 1:1251412/1‑150 (MQ=255) aTACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACgg < 2:778592/131‑1 (MQ=255) aTACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACgg > 1:778592/1‑131 (MQ=255) aTACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAg > 1:106498/1‑150 (MQ=255) ccAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGc < 2:1112772/150‑1 (MQ=255) cAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGCGGcc > 2:1305895/1‑150 (MQ=255) gACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCaa < 2:77307/150‑1 (MQ=255) gcgcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCCCGTGCCAGGGCttt > 2:240427/1‑130 (MQ=255) gcgcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCCCGTGCCAGGGCttt < 1:240427/131‑2 (MQ=255) ttttCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTGGATGAAAGTGGCCAAATCCATTTg > 2:896777/1‑150 (MQ=255) gcgACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGa < 2:1010014/150‑1 (MQ=255) gcgACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGCGGCCAAATGGATTTGGccac < 1:1305895/150‑3 (MQ=255) tCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTc > 1:1066810/1‑150 (MQ=255) tCCATTGGT‑GAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTcc > 2:411554/1‑150 (MQ=255) ccATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTcc < 1:599895/150‑1 (MQ=255) cATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCt > 1:520079/1‑150 (MQ=255) tggtggAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTAt > 1:1032160/1‑150 (MQ=255) gAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGc < 2:520079/150‑1 (MQ=255) aGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCt < 1:237858/150‑1 (MQ=255) gCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGACAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCtt > 1:236105/1‑150 (MQ=255) tctGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCa < 2:687862/150‑1 (MQ=255) tctGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCa > 2:644625/1‑150 (MQ=255) tGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGAt > 1:1220232/1‑97 (MQ=255) tGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGAt < 2:1220232/97‑1 (MQ=255) ttGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACgg < 1:1122584/120‑1 (MQ=255) ttGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACgg > 2:1122584/1‑120 (MQ=255) gCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGctc < 2:1032160/150‑1 (MQ=255) ttCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt > 2:781954/1‑150 (MQ=255) ttCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt > 1:1222688/1‑150 (MQ=255) ttCGAAAGCGATGTGATCGCGCGAGGTCTGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt > 1:903928/1‑150 (MQ=255) cGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCtt < 2:1066810/150‑1 (MQ=255) gATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCttt < 1:781954/150‑1 (MQ=255) tCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCCAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTc > 1:674836/1‑150 (MQ=255) tCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGa > 2:1192331/1‑92 (MQ=255) tCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGa < 1:1192331/92‑1 (MQ=255) gcgcgcAACTCGGTGTATTGAGCGCAAGATAAAGCCCTGGCACGGGCCAGGGCTTCGAAGAAAGTGGCCAAATACATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCtt > 2:602104/1‑150 (MQ=255) gcgcgcAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCtt < 2:1222688/150‑1 (MQ=255) gcgcgcAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCtt < 2:106498/150‑1 (MQ=255) aaaCCCGGGGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCTGCTTTCAGGCCGGCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTaa < 2:236105/149‑1 (MQ=255) aaCCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAc < 1:445393/129‑1 (MQ=255) aaCCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAc > 2:445393/1‑129 (MQ=255) cccGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATgc > 1:1168123/1‑150 (MQ=255) cGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATgcgc > 1:380044/1‑150 (MQ=255) gtgtATTGAGCGCAAGAAAAAGCCCTGGCACGGGCGAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCAc > 2:690024/1‑150 (MQ=255) gtgtATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCAc < 1:644625/150‑1 (MQ=255) aGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTg > 1:960135/1‑150 (MQ=255) aaGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTc < 1:411554/150‑1 (MQ=255) aGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCt > 1:687061/1‑150 (MQ=255) aaaGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCAc < 1:524110/132‑1 (MQ=255) aaaGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCAc > 2:524110/1‑132 (MQ=255) aaGCCCTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTc < 2:1350582/144‑1 (MQ=255) aaGCCCTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTc > 1:1350582/1‑144 (MQ=255) cccTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCg > 1:60079/1‑150 (MQ=255) ccTGGCCCGTGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCgg > 2:4905/1‑150 (MQ=255) gCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAAc < 2:687061/150‑1 (MQ=255) | ATGATGCGGCGTATGACGCGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAAAAAGCCCTGGCACGGGCCAGGGCTTCGATGAAAGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAAC > NC_002947/5351242‑5351537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |