Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,364,611 A→T 11.7% V24D (GTC→GAC)  folP ← dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,364,6110AT11.7% 135.4 / 8.6 50V24D (GTC→GAC) folPdihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base T (2/4);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

CCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGCGTGCCC  >  NC_002947/5364467‑5364756
                                                                                                                                                |                                                                                                                                                 
cgggtgcgcGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg                                                                                                                                              <  1:1394250/145‑1 (MQ=255)
cccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg                                                                                                                                              <  2:403319/150‑1 (MQ=255)
 ccGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATggg                                                                                                                                             >  2:1185366/1‑150 (MQ=255)
    ggCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTAt                                                                                                                                          <  1:996090/150‑1 (MQ=255)
    ggCGCGTGGACGCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTAt                                                                                                                                          <  2:686561/150‑1 (MQ=255)
     gcgcGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATACTGAGAATACCCAAGTCAttcggatt                                                                                                                                         <  2:765784/150‑8 (MQ=255)
      cgcgTGGACTCGCAACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGTCATGGGTAttc                                                                                                                                        <  2:382209/150‑3 (MQ=255)
         gTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGAc                                                                                                                                     >  2:1332336/1‑150 (MQ=255)
         gTGGACCCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTAGTCACGCTGACTCAAGCGACCACCATCGGAGAAGGGATCGCGGGTGATATTGAGAATACCGATGATGTGCGTATgatac                                                                                                                                     >  2:63548/1‑146 (MQ=255)
             aCTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAAt                                                                                                                                 <  2:994857/150‑1 (MQ=255)
                 gCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                                                                                                                        <  2:65481/139‑1 (MQ=255)
                 gCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGc                                                                                                                                        >  1:65481/1‑139 (MQ=255)
                  ccACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTTATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGa                                                                                                                            <  2:1231233/150‑1 (MQ=255)
                       gATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCg                                                                                                                       >  1:210595/1‑150 (MQ=255)
                                            ggcggcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggt                                                                                                  <  1:645863/150‑1 (MQ=255)
                                               ggcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg                                                                                               <  2:210595/150‑1 (MQ=255)
                                                  aaCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggta                                                                                                 <  1:1331587/145‑1 (MQ=255)
                                                  aaCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggta                                                                                                 >  2:1331587/1‑145 (MQ=255)
                                                    ccATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACt                                                                                          >  1:725516/1‑150 (MQ=255)
                                                     cATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg                                                                                                                                              >  2:441998/1‑97 (MQ=255)
                                                     cATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg                                                                                                                                              <  1:441998/97‑1 (MQ=255)
                                                           ttCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGa                                                                                   >  2:292374/1‑150 (MQ=255)
                                                              ggCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCt                                                                                >  2:532108/1‑150 (MQ=255)
                                                                    gcgcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGa                                                                          <  1:1332336/150‑1 (MQ=255)
                                                                    gcgcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGa                                                                          >  2:428524/1‑150 (MQ=255)
                                                                      gcAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATg                                                                        >  1:999294/1‑150 (MQ=255)
                                                                            ccTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATCGCAGCCCTTa                                                                  >  1:60237/1‑150 (MQ=255)
                                                                             cTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAg                                                                 <  1:1185366/150‑1 (MQ=255)
                                                                              tcgtcgCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAgt                                                                <  2:725516/150‑1 (MQ=255)
                                                                                gtcgCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAgtgt                                                              >  2:717372/1‑150 (MQ=255)
                                                                                                cGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGTCATg                                                                                                                                               <  2:837531/53‑1 (MQ=255)
                                                                                                cGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGTCATg                                                                                                                                               >  1:837531/1‑53 (MQ=255)
                                                                                                  aCCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGccgccg                                            >  1:796316/1‑150 (MQ=255)
                                                                                                                 ggAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGa                             <  2:367170/150‑1 (MQ=255)
                                                                                                                   aaTCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACg                           <  1:428524/150‑1 (MQ=255)
                                                                                                                    aTCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGc                          <  1:532108/150‑1 (MQ=255)
                                                                                                                         gggTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATc                     <  1:721289/150‑1 (MQ=255)
                                                                                                                          ggTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAgt                                                                <  1:575174/106‑1 (MQ=255)
                                                                                                                          ggTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAgt                                                                >  2:575174/1‑106 (MQ=255)
                                                                                                                             gATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTgg                 <  1:63548/150‑1 (MQ=255)
                                                                                                                                 ttGAGAATACCCATGACATGGGTATGCGACAAATCAAGaa                                                                                                                           <  1:53237/40‑1 (MQ=255)
                                                                                                                                 ttGAGAATACCCATGACATGGGTATGCGACAAATCAAGaa                                                                                                                           >  2:53237/1‑40 (MQ=255)
                                                                                                                                     gcaTACCCATGTCATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGa                                    <  1:1000075/121‑1 (MQ=255)
                                                                                                                                     gcaTACCCATGTCATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGa                                    >  2:1000075/3‑123 (MQ=255)
                                                                                                                                          cccATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTa                                                                  >  2:666105/1‑88 (MQ=255)
                                                                                                                                          cccATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTa                                                                  <  1:666105/88‑1 (MQ=255)
                                                                                                                                          cccATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTg                  <  2:797067/136‑1 (MQ=255)
                                                                                                                                          cccATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTg                  >  1:797067/1‑136 (MQ=255)
                                                                                                                                           ccATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGCGTGcc   >  2:242319/1‑150 (MQ=255)
                                                                                                                                            cATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGCGTGccc  <  2:1052129/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGGGTACTGCACTGAGCTCATAGATGCCCTTAGTGTTCAGCCGCTGGGCCGCCGATTGGCGATTCCGGACGCTCATCCTGGGCCGCAGGCGTGCCC  >  NC_002947/5364467‑5364756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑