Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,381,316 T→A 10.4% intergenic (+33/+32) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,381,3160TA10.4% 136.4 / 5.9 48intergenic (+33/+32)bamE/PP_4732outer membrane protein assembly factor BamE/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base A (3/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAG  >  NC_002947/5381173‑5381459
                                                                                                                                               |                                                                                                                                               
ccTGAAGAAAAACCGGCCAAGCCTGGTAAGACGGTAGAGTCCATCCAGCTCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCg                                                                                                                                           <  2:13284/150‑1 (MQ=255)
  tGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCTGGAACATCAGATGCAAAAAAGCCAGGACTTGGTCCggg                                                                                                                                         >  1:511880/1‑150 (MQ=255)
      gaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCttt                                                                                                                                     >  2:1290032/1‑150 (MQ=255)
       caaaaCCGGCCGAGCGTGGTTCGACCGAAGAGCCCATCCAGCGCGAGATCGACACCATCGAGACTGCCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCtttt                                                                                                                                    <  2:1075780/149‑1 (MQ=255)
         aaaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGCCCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCtcttta                                                                                                                                  >  1:1075780/1‑149 (MQ=255)
                     ccTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTta                                                                                                                      <  1:1040192/150‑1 (MQ=255)
                       tGGTTTGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtata                                                                                                                    <  1:1123862/150‑1 (MQ=255)
                              aCCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAgg                                                                                                             >  2:1421780/1‑150 (MQ=255)
                              aCCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAgg                                                                                                             <  2:206982/150‑1 (MQ=255)
                               ccGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGc                                                                                                            <  2:251259/150‑1 (MQ=255)
                                cGAAGAGTCCATCCAGCGCGAGATCGACACCATGGAGACTACGCCGGTCCAGACCCCGGCACCGCTGGAAACGCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGATTTGGTCCGGGCTTTTTTATTTGCAGGTTGTACggcaggca                                                                                                           >  2:682467/1‑150 (MQ=255)
                                cGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCa                                                                                                           <  2:363695/150‑1 (MQ=255)
                                             caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTgg                                                                                              <  1:354510/150‑1 (MQ=255)
                                                       tCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTATGCCGCTGGTAGCTGAGTCATTCAATTGCTTGGCACGCTGGCa                                                                                    >  2:824746/1‑150 (MQ=255)
                                                         gACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTgg                                                                                                                                               >  1:230484/1‑89 (MQ=255)
                                                         gACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTgg                                                                                                                                               <  2:230484/89‑1 (MQ=255)
                                                              cATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCa                                                                             >  2:1205849/1‑150 (MQ=255)
                                                                     aCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTgg                                                                                      >  2:1410301/1‑134 (MQ=255)
                                                                     aCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTgg                                                                                      <  1:1410301/134‑1 (MQ=255)
                                                                         ccccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCaa                                                                                                      <  2:1317073/114‑1 (MQ=255)
                                                                         ccccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCaa                                                                                                      >  1:1317073/1‑114 (MQ=255)
                                                                          cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                          >  2:1236348/1‑77 (MQ=255)
                                                                          cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                          <  1:1236348/77‑1 (MQ=255)
                                                                          cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGTCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTa                                                                 <  2:119024/150‑1 (MQ=255)
                                                                          cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCTTa                                                                 <  1:1421780/150‑1 (MQ=255)
                                                                            cGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTAtt                                                               >  1:790033/1‑150 (MQ=255)
                                                                                 ccGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcgccg                                                          >  2:1052492/1‑150 (MQ=255)
                                                                                       ccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGAt                                                    >  2:1035641/1‑150 (MQ=255)
                                                                                           cACCGCCGGAAAACCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGaa                                                <  1:167511/150‑1 (MQ=255)
                                                                                             ccGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTCGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGCCAGATAGCATCGACCAGCGTATTCGCCGCCTGATTGAATa                                              <  1:824746/150‑1 (MQ=255)
                                                                                                cTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACTCTGGCAGAACGCATCGACCAGCGTATTTGCCGCCTGATTAGATAGCg                                           >  1:227623/1‑150 (MQ=255)
                                                                                                   gAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGc                                        >  2:185042/1‑150 (MQ=255)
                                                                                                    aaaCCCGCTAAGCGGGAACATCGGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGcc                                       >  1:45803/1‑150 (MQ=255)
                                                                                                     ttcccGCTTAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGccc                                      >  2:96508/3‑150 (MQ=255)
                                                                                                          gCTAATCGGGAACATCAGATGCAAAAAAGCCCGGACTTCGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGt                                 <  1:682467/150‑1 (MQ=255)
                                                                                                                       aTCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATc                    <  2:1257558/150‑1 (MQ=255)
                                                                                                                         cAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCgg                  <  1:96508/150‑1 (MQ=255)
                                                                                                                           gATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGcc                >  2:499680/1‑150 (MQ=255)
                                                                                                                             tGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATATCGGGCCCAACGTGGCGCGCACAATCGGCCCg              <  1:630334/150‑1 (MQ=255)
                                                                                                                             tGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCggg                                         <  1:842351/123‑1 (MQ=255)
                                                                                                                             tGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCggg                                         >  2:842351/1‑123 (MQ=255)
                                                                                                                                aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCg           >  2:707936/1‑150 (MQ=255)
                                                                                                                                  aaaaGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTa         >  1:765772/1‑150 (MQ=255)
                                                                                                                                    aaGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                               >  1:926614/1‑62 (MQ=25)
                                                                                                                                    aaGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                               <  2:926614/62‑1 (MQ=25)
                                                                                                                                      gCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCg     >  1:869772/1‑150 (MQ=255)
                                                                                                                                        ccGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGaa   <  1:707936/150‑1 (MQ=255)
                                                                                                                                         cGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAg  <  2:511880/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAG  >  NC_002947/5381173‑5381459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑