Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,390,635 T→G 10.7% intergenic (+676/‑46) PP_4738 → / → PP_mr56 hypothetical protein/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,390,6350TG10.7% 119.4 / 7.6 48intergenic (+676/‑46)PP_4738/PP_mr56hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base G (2/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGG  >  NC_002947/5390487‑5390783
                                                                                                                                                    |                                                                                                                                                    
cTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcatc                                                                                                                                                     <  1:164465/150‑1 (MQ=255)
           ccTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCt                                                                                                                                          >  1:1281748/1‑150 (MQ=255)
            cTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcatc                                                                                                                                                     >  2:494104/1‑138 (MQ=255)
            cTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCtcatc                                                                                                                                                     <  1:494104/138‑1 (MQ=255)
            cTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCATGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCgg                                                                                                                                            <  1:1250605/147‑1 (MQ=255)
            cTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCATGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCgg                                                                                                                                            >  2:1250605/1‑147 (MQ=255)
             tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTTCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                        >  1:299504/1‑150 (MQ=255)
             tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                        >  1:822731/1‑150 (MQ=255)
             tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                               <  1:754644/143‑1 (MQ=255)
             tGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTACGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                               >  2:754644/1‑143 (MQ=255)
             tGGAATCTGGGCGTTGTGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCttt                                                                                                                                        <  2:968635/150‑1 (MQ=255)
              ggAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTg                                                                                                                                       >  2:471638/1‑150 (MQ=255)
               gAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGa                                                                                                                                      <  1:1144866/150‑1 (MQ=255)
               gAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCACCATTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGa                                                                                                                                      <  1:1397392/150‑1 (MQ=255)
                               ggTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATac                                                                                                                      <  1:471638/150‑1 (MQ=255)
                                      aaaTCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAgcgc                                                                                                               <  1:230036/150‑1 (MQ=255)
                                         tCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCa                                                                                                            <  1:591304/150‑1 (MQ=255)
                                                  aCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGAc                                                                                                                                     <  2:176986/116‑1 (MQ=255)
                                                  aCCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGAc                                                                                                                                     >  1:176986/1‑116 (MQ=255)
                                                   ccccTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCa                                                                                                  >  2:445044/1‑150 (MQ=255)
                                                      cTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATgg                                                                                               >  1:540415/1‑150 (MQ=255)
                                                        ttCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCg                                                                                             >  2:715530/1‑150 (MQ=255)
                                                         tCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCgg                                                                                            <  1:541521/150‑1 (MQ=255)
                                                            tGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGGTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTtg                                                                                         <  1:234659/150‑1 (MQ=255)
                                                                 ggCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                               <  1:212525/91‑1 (MQ=255)
                                                                 ggCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGATGAGCAACGAc                                                                                                                                               >  2:212525/1‑91 (MQ=255)
                                                                         ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACg                                                                            >  1:544302/1‑150 (MQ=255)
                                                                         ccAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACg                                                                            >  1:128839/1‑150 (MQ=255)
                                                                                            cTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                         <  2:540415/150‑1 (MQ=255)
                                                                                               ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCAt                                                                                    >  2:423963/1‑120 (MQ=255)
                                                                                               ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCAt                                                                                    <  1:423963/120‑1 (MQ=255)
                                                                                               tttgCCGTTGCGGTCATTTTTTCAGCGTTTTAGTCTCGGTGGGTCGTTGCCCATCAACGACCGGCTTTGACCGGCTGCTACCAAACACAGAGCAGTTAGCCACATAGGGCGGGTGTGCATGTGGCACGCTTGCGTGCGCCGGTTTCtgtg                                                      <  1:387409/150‑1 (MQ=255)
                                                                                                  tCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTc                                                   <  1:445044/150‑1 (MQ=255)
                                                                                                      ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                               >  1:597337/1‑150 (MQ=255)
                                                                                                         cGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCACCAACCCCGGGCTTTGACAGGCCGCAAAGATAAACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTc                                            <  1:674517/150‑1 (MQ=255)
                                                                                                           gTCATTTTTTTAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTg                                          >  2:1154400/1‑150 (MQ=255)
                                                                                                           gTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTg                                          >  2:1080712/1‑150 (MQ=255)
                                                                                                            tCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTAATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                         >  1:871325/1‑150 (MQ=255)
                                                                                                             cATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTAATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTc                                        <  2:871325/150‑1 (MQ=255)
                                                                                                              aTTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCa                                       >  1:683186/1‑150 (MQ=255)
                                                                                                               ttttttCAGGGTTTTAGTCTCGGTGGGTCGTTGTTTATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                                      <  2:335038/150‑1 (MQ=255)
                                                                                                               ttttttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCaa                                      <  2:822731/150‑1 (MQ=255)
                                                                                                                   ttCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCa                                  <  2:597337/150‑1 (MQ=255)
                                                                                                                       gggTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGca                              >  2:1132397/1‑150 (MQ=255)
                                                                                                                                tctcGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca                     >  2:503541/1‑150 (MQ=255)
                                                                                                                                     gccggTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAtt                >  1:796502/4‑150 (MQ=255)
                                                                                                                                       gggTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTcc              <  2:128839/150‑1 (MQ=255)
                                                                                                                                         gTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAagcgtgccccat                                                              >  2:594493/1‑95 (MQ=255)
                                                                                                                                         gTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAagcgtgccccat                                                              <  1:594493/100‑6 (MQ=255)
                                                                                                                                         gTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCAt                                                                                                 <  2:832271/65‑1 (MQ=37)
                                                                                                                                            gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTg         >  1:862958/1‑150 (MQ=255)
                                                                                                                                                 tcatcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGc    <  2:796502/150‑1 (MQ=255)
                                                                                                                                                   atcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCgg  >  2:248520/1‑150 (MQ=255)
                                                                                                                                                   atcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCgg  <  1:1080712/150‑1 (MQ=255)
                                                                                                                                                    tcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGa        <  2:1076370/143‑1 (MQ=255)
                                                                                                                                                    tcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGa        >  1:1076370/1‑143 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGG  >  NC_002947/5390487‑5390783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑