| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,482,349 | G→C | 8.6% | G76A (GGC→GCC) | dusB → | tRNA dihydrouridine synthase DusB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,482,349 | 0 | G | C | 8.6% | 167.1 / 7.9 | 58 | G76A (GGC→GCC) | dusB | tRNA dihydrouridine synthase DusB |
| Reads supporting (aligned to +/- strand): ref base G (25/28); new base C (2/3); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
CCTTTCCGTACACTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGAAGCCTTGGT > NC_002947/5482201‑5482493 | ccTTTCCGTACACTTTGCCAGCGCCCGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCcggc > 2:360641/1‑150 (MQ=255) ccGTACACTTTGCCAGCGCATGGGCGACGGAATGGTGGTGTCGGAAATGGTTAGCAGAGACATGAGCCTGTGGAAAAGCCGAAAGTCGAGGCTGCGCCGCATCCATGAAGGTGATCGCGAGGCACGCAAGGTGCAGATCGCCGGCGGCGa > 2:248176/1‑150 (MQ=255) gTACACTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATg > 1:839358/1‑150 (MQ=255) gTACACTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATg > 1:16571/1‑150 (MQ=255) acTTTGCCAGCTCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCGCATCGCCGCCGGCGAtctgca < 1:1129769/150‑6 (MQ=255) acTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAATTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCGCATCGCCGCCGGCGAtctgca < 2:829805/150‑6 (MQ=255) acTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCa < 1:283517/150‑1 (MQ=255) acTGTGCCCGCGCCTGGCCGCCGGTAGGGTGGTGTCGGAAATGGTTAGCAGCGAGATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCa < 2:423561/150‑1 (MQ=255) gcgcCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGc < 1:607819/150‑1 (MQ=255) gcCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTg > 2:204974/1‑150 (MQ=255) tGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTgcgg > 2:329246/1‑150 (MQ=255) tGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTgcgg > 1:1233801/1‑150 (MQ=255) ggCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTgcggcg > 2:381545/1‑150 (MQ=255) ggCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTgcggcg < 2:169081/150‑1 (MQ=255) gcgcCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGcggcgg > 2:870052/1‑150 (MQ=255) cGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCaaa < 1:800640/150‑1 (MQ=255) gTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAgg > 2:863782/1‑150 (MQ=255) tggtgTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGCGGATGCGCAGATGATGGCTGCGGCGGCAAAGGACAATg > 2:847796/1‑150 (MQ=255) tCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGGGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAg < 2:939553/150‑1 (MQ=255) aaaTGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCgg < 1:381545/150‑1 (MQ=255) aTGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGt < 2:16571/150‑1 (MQ=255) ttAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGAGGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGccc > 1:206499/1‑150 (MQ=255) agcagcGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAg < 2:206499/150‑1 (MQ=255) cagcGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGCCGGCGAtct > 2:773725/1‑97 (MQ=255) cagcGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGCCGGCGAtct < 1:773725/99‑3 (MQ=255) gcGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGatca > 1:871033/1‑150 (MQ=255) gACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGatcatc < 2:1265278/150‑1 (MQ=255) gACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGatcatc < 2:1325655/150‑1 (MQ=255) aaCATGAGCCGGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGatcatc < 2:568205/149‑1 (MQ=255) aCATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCg < 2:432074/150‑1 (MQ=255) cATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGa < 1:863782/150‑1 (MQ=255) aTGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGat > 1:120160/1‑150 (MQ=255) gAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCAACGCTCGGTGCAGATCGCCGGCGGCGATGCTCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGatat < 1:884451/150‑1 (MQ=255) cTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAAc > 1:1126619/1‑150 (MQ=255) ggAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATgg < 2:1126619/150‑1 (MQ=255) gAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATggg < 2:228741/150‑1 (MQ=255) gAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATggg < 2:839358/150‑1 (MQ=255) gAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATggg < 1:762841/150‑1 (MQ=255) cAGCCGCAAGTCGAGCCTGCGCCGCATACAAGAAGGTGATTCCGGATGCCACCAAGTGATAATCGCCAGCGTCTATTCGCAGATGATGGCTGCGGCGGCAAAGGCCCATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTg > 1:26764/1‑150 (MQ=255) ccGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGTTCAGCGATATCAACATTGGGTGTcc > 2:784963/1‑150 (MQ=255) tGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCt < 2:999171/150‑1 (MQ=255) aTGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCg > 2:1332942/1‑150 (MQ=255) gTGATCCCGAGCCACGCTCGGTGGAGGGCGGTGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGct < 1:360641/150‑1 (MQ=255) aTCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTg > 2:890119/1‑150 (MQ=255) ccGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCtt < 2:1233801/150‑1 (MQ=255) cACGCTCGGTGCAGATCGCCGGCGGTGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTga > 2:15/1‑150 (MQ=255) cACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGTTGTCAGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTga < 2:655325/150‑1 (MQ=255) aCGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTgag < 1:204974/150‑1 (MQ=255) gCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGa < 2:848611/85‑1 (MQ=255) gCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGa > 1:848611/1‑85 (MQ=255) tCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGAt > 2:181887/1‑150 (MQ=255) tctgcgcaTCGCCGCCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGa > 1:826359/8‑150 (MQ=255) ggTGTAGATCACCGGCGGCGATTTGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGa > 2:1380349/1‑150 (MQ=255) gTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGaa > 2:962939/1‑150 (MQ=255) gTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCAATCGTGCAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGaa > 2:1058753/1‑150 (MQ=255) tGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAACACGGTGGCTCTGCTTTATTGAGAGATGAAg < 1:970288/150‑1 (MQ=255) aGATTGCCGGAGGCGATGCAAAAATGATGGCTGCGGCGGAAAAGGCTAATGTCAAGGCGAGTGCAAAGATAATCGATATCAACATGGAAAAAAAAAAAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGAAGCCt < 2:471986/150‑1 (MQ=255) gATCGCGGGCGGCGTTGCGCATATGATGGCTGCGGCGGCAAAGGCAAATGTCGAGGCGGGGGCCTAGATCAACGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGAAGCCtt < 2:26764/150‑1 (MQ=255) cGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGAAGCCTTGGt > 2:196050/1‑150 (MQ=255) | CCTTTCCGTACACTTTGCCAGCGCCTGGGCGCCGGTATGGTGGTGTCGGAAATGGTTAGCAGCGACATGAGCCTGTGGAACAGCCGCAAGTCGAGCCTGCGCCGCATCCATGAAGGTGATCCCGAGCCACGCTCGGTGCAGATCGCCGGCGGCGATGCGCAGATGATGGCTGCGGCGGCAAAGGCCAATGTCGAGGCGGGTGCCCAGATCATCGATATCAACATGGGCTGTCCGGCAAAAAAAGTCTGCAACAAAGCCGCAGGCTCTGCTTTATTGAGAGATGAAGCCTTGGT > NC_002947/5482201‑5482493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |