Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,486,554 C→G 8.2% I422M (ATC→ATG)  purD → phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,486,5540CG 8.2% 153.1 / 5.1 49I422M (ATC→ATG) purDphosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GCAAGGTAACCACCAACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACTTGGCGCGCCGCGCATAGTGA  >  NC_002947/5486406‑5486685
                                                                                                                                                    |                                                                                                                                   
gCAAGGTAACCACCAACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACTACAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCg                                                                                                                                    >  1:513001/1‑150 (MQ=255)
           accaACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGAAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAgcgc                                                                                                                         >  1:39975/1‑150 (MQ=255)
             caACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTCGCGGGCGATGGCCcgata                                                                                                                             >  2:465945/1‑141 (MQ=255)
             caACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTCGCGGGCGATGGCCcgata                                                                                                                             <  1:465945/144‑4 (MQ=255)
              aaCGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGt                                                                                                                      <  1:1371683/150‑1 (MQ=255)
              aaCGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGt                                                                                                                      >  2:716252/1‑150 (MQ=255)
                    gggcgCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCAc                                                                                                                <  2:629377/150‑1 (MQ=255)
                     ggcgCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCAcc                                                                                                               >  2:497683/1‑150 (MQ=255)
                      gcgcGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACca                                                                                                              <  2:1382409/150‑1 (MQ=255)
                             cTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCagtaa                                                                                                       <  1:11431/150‑1 (MQ=255)
                               gtgtgCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCagtaagt                                                                                                     >  2:673719/1‑150 (MQ=255)
                               gtgtgCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCagtaa                                                                                                       <  1:1269991/148‑1 (MQ=255)
                               gtgtgCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCagtaa                                                                                                       >  2:1269991/1‑148 (MQ=255)
                                  tgCCACCGCCATGGGCAGCACCGTTGCCGACGTGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGa                                                                                                                             <  2:569256/123‑1 (MQ=255)
                                  tgCCACCGCCATGGGCAGCACCGTTGCCGACGTGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGa                                                                                                                             >  1:569256/1‑123 (MQ=255)
                                    ccACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGtactac                                                                                                <  1:814365/150‑1 (MQ=255)
                                      aCCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCg                                                                                              <  1:1376407/150‑1 (MQ=255)
                                       ccGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCgg                                                                                             >  2:3222/1‑150 (MQ=255)
                                          ccATGGGCAGAACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTAt                                                                                          <  2:295987/150‑1 (MQ=255)
                                                 cagcaCCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTa                                                                                    >  2:1274889/1‑149 (MQ=255)
                                                 cagcaCCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTa                                                                                    <  1:1274889/149‑1 (MQ=255)
                                                           gCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGc                                                                         <  1:497683/150‑1 (MQ=255)
                                                             cGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCt                                                                       <  2:296634/150‑1 (MQ=255)
                                                              gACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTg                                                                      >  2:848004/1‑150 (MQ=255)
                                                                    cagcagcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGc                                                                                        >  2:877447/1‑126 (MQ=255)
                                                                    cagcagcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGc                                                                                        <  1:877447/126‑1 (MQ=255)
                                                                     agcagcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTg                                                                      <  1:243693/143‑1 (MQ=255)
                                                                     agcagcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTg                                                                      >  2:243693/1‑143 (MQ=255)
                                                                     agcagcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATc                                                               <  2:1398496/150‑1 (MQ=255)
                                                                         gcagGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGc                                                           >  1:740800/1‑150 (MQ=255)
                                                                              cTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCtcgtc                                                      >  1:896866/1‑150 (MQ=255)
                                                                                     ccTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTCTGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAggg                                               >  2:1029023/1‑150 (MQ=255)
                                                                                            aaGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTc                                                                            <  2:560662/114‑1 (MQ=255)
                                                                                            aaGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTc                                                                            >  1:560662/1‑114 (MQ=255)
                                                                                            aaGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCGATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGaa                                        <  1:1373212/150‑1 (MQ=255)
                                                                                              ggAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATg                                      <  2:39975/150‑1 (MQ=255)
                                                                                                      gCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGgccttgc                              <  1:716252/150‑1 (MQ=255)
                                                                                                       cTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCg                                                                                      <  2:369169/93‑1 (MQ=255)
                                                                                                       cTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCg                                                                                      >  1:369169/1‑93 (MQ=255)
                                                                                                       cTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGgccttgcc                             >  1:896820/1‑150 (MQ=255)
                                                                                                       cTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGgccttgcc                             <  1:258539/150‑1 (MQ=255)
                                                                                                               gCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACtt                     >  2:721580/1‑150 (MQ=255)
                                                                                                                        aCCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACTTGGcgcgccg            >  1:120361/1‑150 (MQ=255)
                                                                                                                                gACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACTTGGCGCGCCGCGCATAGt    <  1:3222/150‑1 (MQ=255)
                                                                                                                                  cATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACTTGGCGCGCCGCGCATAGTGa  <  2:740800/150‑1 (MQ=255)
                                                                                                                                      ggCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGgc                                   >  2:766793/1‑113 (MQ=255)
                                                                                                                                      ggCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGgc                                   <  1:766793/113‑1 (MQ=255)
                                                                                                                                         tcgcGGGCGATGGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGc                                                           <  2:612382/83‑1 (MQ=255)
                                                                                                                                         tcgcGGGCGATGGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGc                                                           >  1:612382/4‑86 (MQ=255)
                                                                                                                                                    |                                                                                                                                   
GCAAGGTAACCACCAACGGCGGGCGCGTGCTGTGTGCCACCGCCATGGGCAGCACCGTTGCCGACGCGCAGCAGCAGGCTTATCGCCTGGCCAAGGAAGTCAGCTGGAACGGCAGCTTCTACCGTTCCGACATCGGCTATCGGGCCATCGCCCGCGAGCGCGGTGAGCACCAGCAGTAAGTACTACCGGTATGCCGTAGCGGTTTCAGCCTGCGGCATCCGGCTCGTCGACAGGGCCCGGAATGGGCCTTGCCTTCGACTTGGCGCGCCGCGCATAGTGA  >  NC_002947/5486406‑5486685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑