Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,507,652 G→C 11.3% A289P (GCG→CCG)  cycA → D‑alanine/beta‑alanine/D‑serine/glycine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,507,6520GC11.3% 127.1 / 7.1 46A289P (GCG→CCG) cycAD‑alanine/beta‑alanine/D‑serine/glycine permease
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base C (2/3);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCCGCGGGCGATCAACTCGATCCCGATCCGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCG  >  NC_002947/5507507‑5507798
                                                                                                                                                  |                                                                                                                                                  
gCCGCGGGCGATCAACTCGATCCCGATCCGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCcggcggc                                                                                                                                                <  1:1205398/150‑1 (MQ=255)
   gcgGGCGATCAACTCGATCCCGATCCGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCACGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCggcggcggc                                                                                                                                             <  1:537377/150‑1 (MQ=255)
       gCGATCAACTCGATCCCGATCCGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGCCGCCGGCAAGc                                                                                                                                         <  2:73489/150‑1 (MQ=255)
              aCTCGATCCCGATCCGTATCATCGTGTTCGATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGAAACCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGATGTTAGATCTGAAGGGCGTGCAGGAGGCGGCAAGCAtcatca                                                                                                                                  >  2:472889/1‑150 (MQ=255)
               cTCGACCCCGATCCGTATCATCGTGTTCTATGTGCT‑GCGTGGAATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCaa                                                                                                                                 <  1:374376/150‑1 (MQ=255)
                   atcccgatccGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTc                                                                                                                             >  2:1273460/1‑150 (MQ=255)
                    tcccgatccGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCg                                                                                                                            >  1:1015427/1‑150 (MQ=255)
                      ccgatccGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCgtg                                                                                                                          <  2:250318/150‑1 (MQ=255)
                         atccATATCAGCGTGTGCTATGTGCGGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCgtggtg                                                                                                                       >  2:1090154/1‑150 (MQ=255)
                                 cctcGTATTCAATGTGCTGGCGCTG‑ATCGCGCTCATAGACATGAACCCGTGGCGCGATGTGGTGCCCGGAAAAAGCAAGTTCGTTGCGTTGTTCGTGCTGGCAGGCTTGAAGACGGCGGCAAACATAATCAACTTCGTGGTGCTGACCTc                                                                                                               >  2:1360803/3‑150 (MQ=255)
                                  atcGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGc                                                                                                                      <  1:1176639/142‑1 (MQ=255)
                                  atcGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGc                                                                                                                      >  2:1176639/1‑142 (MQ=255)
                                                 cTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGcc                                                                                               >  1:821495/1‑150 (MQ=255)
                                                    gcgcTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTtcgtc                                                                                                   <  1:423718/143‑1 (MQ=255)
                                                    gcgcTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTtcgtc                                                                                                   >  2:423718/1‑143 (MQ=255)
                                                    gcgcTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAAc                                                                                            >  1:853230/1‑150 (MQ=255)
                                                             cgcgATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCgt                                                                                    <  1:1273460/150‑1 (MQ=255)
                                                                gATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGtt                                                                                 <  2:817646/150‑1 (MQ=255)
                                                                   cATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTtctc                                                                              <  1:591290/150‑1 (MQ=255)
                                                                     tGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCTTCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTcca                                                                            <  1:982851/150‑1 (MQ=255)
                                                                     tGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTcca                                                                            <  1:983031/150‑1 (MQ=255)
                                                                               cccGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCAt                                                                  >  1:1144575/1‑150 (MQ=255)
                                                                                ccGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCgtg                                                                                                                          >  1:1227179/1‑93 (MQ=255)
                                                                                ccGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCgtg                                                                                                                          <  2:1227179/93‑1 (MQ=255)
                                                                                              ggtgCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTc                                                   <  2:1144575/150‑1 (MQ=255)
                                                                                                     ggAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATg                                                                 <  1:1044054/129‑1 (MQ=255)
                                                                                                     ggAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATg                                                                 >  2:1044054/1‑129 (MQ=255)
                                                                                                      gAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGct                                                               <  2:59434/130‑1 (MQ=255)
                                                                                                      gAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGct                                                               >  1:59434/1‑130 (MQ=255)
                                                                                                          aaGCCCGTCCGTCGAGATCTACGTGCGTGCATGCTTTCCTTCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGa                                       <  2:1015427/150‑1 (MQ=255)
                                                                                                             cccGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTcggc                                                                                                            <  1:1403712/78‑1 (MQ=255)
                                                                                                             cccGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTcggc                                                                                                            >  2:1403712/1‑78 (MQ=255)
                                                                                                              ccGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGGTTCGTCGGCCAACAGCGGCGTGTTCTCCACCCGCCGCATGCTCTACGGCCGGTCGCAGGAAGGTGAcgcg                                   >  2:379692/1‑150 (MQ=255)
                                                                                                              ccGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTtcgtc                                                                                                   <  2:284523/86‑1 (MQ=255)
                                                                                                              ccGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTtcgtc                                                                                                   >  1:284523/1‑86 (MQ=255)
                                                                                                                 ttcgttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGccc                                >  2:1376117/1‑150 (MQ=255)
                                                                                                                    gttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACAGCCTGTCGCAGGAAGGTTACGCGCCCAAg                             >  2:249552/1‑150 (MQ=255)
                                                                                                                       taGTTGTTCCTGCTGGCAGCCTGGCCGGTGGCGGCAAGAATCATCAACTTCGTTGTGCTGACCTCGGTGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCGATACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCt                          <  1:472889/149‑1 (MQ=255)
                                                                                                                          ttgttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTc                       <  1:1326135/150‑1 (MQ=255)
                                                                                                                          ttgttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTc                       >  2:1333980/1‑150 (MQ=255)
                                                                                                                             ttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCgag                    >  1:676245/1‑150 (MQ=255)
                                                                                                                                    tGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGt             >  1:549588/1‑150 (MQ=255)
                                                                                                                                      tgatgCTTGCCGCCGCCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGcc                                                       >  1:796000/5‑106 (MQ=255)
                                                                                                                                      tgatgCTTGCCGCCGCCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGcc                                                       <  2:796000/102‑1 (MQ=255)
                                                                                                                                         tgCTTGCCGCCGCCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGt                                                    >  2:806229/2‑106 (MQ=255)
                                                                                                                                         tgCTTGCCGCCGCCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGt                                                    <  1:806229/105‑1 (MQ=255)
                                                                                                                                          gCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGcc       >  1:646290/1‑150 (MQ=255)
                                                                                                                                               ccGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCg  <  2:853230/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCGCGGGCGATCAACTCGATCCCGATCCGTATCATCGTGTTCTATGTGCTGGCGCTG‑ATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCG  >  NC_002947/5507507‑5507798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑