Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,548,747	T→G	6.0%	M119L (ATG→CTG)	rnr ←	exoribonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,548,747	0	T	G	6.0%	189.4 / 4.2	67	M119L (ATG→CTG)	rnr	exoribonuclease R
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

AGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATA  >  NC_002947/5548600‑5548886                                                                                                                                                    |                                                                                                                                            aGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCt                                                                                                                                           >  2:887513/1‑150 (MQ=255)         acgccGTCCTCGGAAAAGCGCACGACCACGTATTCGTTGTCACGGGAGATGACTTCGACCAGCACGCATTCACGGCGGCAAGGGGGGTTGACACCGGAGACGAAGGCAAAGCAGCGGTCGCCGTTGAACACCGGTCGCATCTACGACggg                                                                                                                                   <  2:316710/149‑1 (MQ=255)          cgccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGc                                                                                                                                  >  1:1421/1‑150 (MQ=255)           gccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGActg                                                                                                                                    >  2:997775/1‑145 (MQ=255)           gccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGActg                                                                                                                                    <  1:997775/147‑3 (MQ=255)             cttcttCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGa                                                                                                                               <  1:45590/150‑1 (MQ=255)                 ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGCGGGGCCGTGGaa                                                                                                                           >  2:304360/1‑150 (MQ=255)                 ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGaa                                                                                                                           >  2:600906/1‑150 (MQ=255)                 ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGaa                                                                                                                           >  1:170748/1‑150 (MQ=255)                    gAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                        <  1:226505/150‑1 (MQ=255)                    gAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                        <  1:304360/150‑1 (MQ=255)                         aGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCtt                                                                                                                   <  1:887513/150‑1 (MQ=255)                                   cACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTc                                                                                                         >  1:370644/1‑150 (MQ=255)                                        tttCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCgg                                                                                                    >  2:232627/1‑150 (MQ=255)                                        tttCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAAACGCGGACCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACAGGCTGAGGAACAGATCTTCGATGCCATCATCgg                                                                                                    >  2:144878/1‑150 (MQ=255)                                              gggCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAg                                                                                                                              >  1:848467/1‑118 (MQ=255)                                              gggCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAg                                                                                                                              <  2:848467/118‑1 (MQ=255)                                              gggCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATaa                                                                                              <  2:1421/150‑1 (MQ=255)                                               ggCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAg                                                                                             <  2:370644/150‑1 (MQ=255)                                                gCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAgg                                                                                            <  1:232627/150‑1 (MQ=255)                                                  aCGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                          <  1:966418/150‑1 (MQ=255)                                                  aCGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                          <  1:301251/150‑1 (MQ=255)                                                  aCGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                          <  2:170748/150‑1 (MQ=255)                                                  aCGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                          >  1:81968/1‑150 (MQ=255)                                                   cGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaag                                                                                         <  1:869804/150‑1 (MQ=255)                                                    gggAGATGCCTTCGACCAGACCGCCTTCACTGCTTCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagc                                                                                        <  2:1015940/150‑1 (MQ=255)                                                    gggAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCgg                                                                                                    >  2:318033/1‑138 (MQ=255)                                                    gggAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCgg                                                                                                    <  1:318033/138‑1 (MQ=255)                                                        gaTGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagccgaa                                                                                    <  2:1427302/150‑1 (MQ=255)                                                        gaTGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagccgaa                                                                                    <  1:600906/150‑1 (MQ=255)                                                         aTGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACgg                                                                                                                                    >  1:1032452/1‑101 (MQ=255)                                                         aTGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACgg                                                                                                                                    <  2:1032452/101‑1 (MQ=255)                                                           gACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagccgaagcc                                                                                 >  2:1100747/1‑150 (MQ=255)                                                              ttCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTc                                                                              >  2:831404/1‑150 (MQ=255)                                                              ttCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGACtggt                                                                                                                                  <  1:837801/98‑2 (MQ=255)                                                              ttCGACCAGCACGCCTTAACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGActggt                                                                                                                                  >  2:837801/1‑94 (MQ=255)                                                                  aCCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCgg                                                                          >  1:1134435/1‑150 (MQ=255)                                                                       cACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGcc                                                                     >  1:476802/1‑150 (MQ=255)                                                                            cTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATc                                                                                                      <  2:712488/112‑1 (MQ=255)                                                                            cTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATc                                                                                                      >  1:712488/1‑112 (MQ=255)                                                                            cTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGgaga                                                                >  2:1108569/1‑150 (MQ=255)                                                                             ttCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGAc                                                               >  2:1166436/1‑150 (MQ=255)                                                                                  ggcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGAc                                                          >  1:497167/1‑150 (MQ=255)                                                                                  ggcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGAc                                                          <  1:693207/150‑1 (MQ=255)                                                                                       ccACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAg                                                     <  1:1108569/150‑1 (MQ=255)                                                                                        cACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGa                                                    >  2:669666/1‑150 (MQ=255)                                                                                          cggcggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATc                                                  <  2:1134435/150‑1 (MQ=255)                                                                                             cggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAgg                                               <  2:476802/150‑1 (MQ=255)                                                                                               gTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTc                                             >  1:1286746/1‑150 (MQ=255)                                                                                                tCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                        >  2:1371080/1‑74 (MQ=255)                                                                                                tCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                        <  1:1371080/74‑1 (MQ=255)                                                                                                tCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTcc                                            >  2:875581/1‑150 (MQ=255)                                                                                                      ccGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTg                                      <  1:875581/150‑1 (MQ=255)                                                                                                         gagaCGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCt                                   >  2:1152669/1‑150 (MQ=255)                                                                                                         gagaCGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCt                                   >  2:1153231/1‑150 (MQ=255)                                                                                                              gcgGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCCGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCgg                              >  2:1171328/1‑150 (MQ=255)                                                                                                               cgGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCggg                             <  2:825977/150‑1 (MQ=255)                                                                                                                            ggTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAAGAAGGCAGATCAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCcgc                <  1:810224/150‑1 (MQ=255)                                                                                                                                 ccGTCGAACCACCGTGGTATCTGCGGCGGGGGGCGGTACTGGTCTTCGCGGCGGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGTGTCGTGACCCCGGAGCGGGTCCACCCTGTCGAACCGGGCGTAAGAGCCGcggcgg           <  2:289783/150‑1 (MQ=255)                                                                                                                                   gTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGt         >  1:1264996/1‑150 (MQ=255)                                                                                                                                      gAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGtata      >  2:151294/1‑150 (MQ=255)                                                                                                                                      gAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGtata      <  1:669666/150‑1 (MQ=255)                                                                                                                                        accccAGCCGCCTCTGCGACGCGCTGTGGAACAGGTCTTCGCTGCCGTCCTCGGGAATAAGGAAGCCGAATCCGTCGCGCTGGCCGGAGACCCGACCACAGAGCAGGCCCAGCTTGTCTCCCGCGGCATAAGTGCCGCGCCGCGTATAGa    <  2:93608/147‑1 (MQ=255)                                                                                                                                         cacCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGTCGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGAt   <  2:321570/150‑1 (MQ=255)                                                                                                                                          acCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATa  <  1:1166436/150‑1 (MQ=255)                                                                                                                                              gTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGAcgc                                                             <  2:1163020/87‑1 (MQ=255)                                                                                                                                              gTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGAcgc                                                             >  1:1163020/1‑87 (MQ=255)                                                                                                                                                    |                                                                                                                                            AGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACGCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGGTGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATA  >  NC_002947/5548600‑5548886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑