Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,609,045 G→C 8.7% R202G (CGC→GGC)  waaA ← 3‑deoxy‑D‑manno‑octulosonic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,609,0450GC 8.7% 138.5 / 5.2 46R202G (CGC→GGC) waaA3‑deoxy‑D‑manno‑octulosonic acid transferase
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base C (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GATCAAGGCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCA  >  NC_002947/5608896‑5609194
                                                                                                                                                     |                                                                                                                                                     
gATCAAGGCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCAcgcg                                                                                                                                                       >  1:32011/1‑150 (MQ=255)
          tCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                             <  1:1272037/150‑1 (MQ=255)
                       gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCAc                                                                                                                                <  2:892125/150‑1 (MQ=255)
                       gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCAc                                                                                                                                >  1:1296011/1‑150 (MQ=255)
                        ggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                               >  1:545738/1‑150 (MQ=255)
                             tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                         >  1:1232541/1‑134 (MQ=255)
                             tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                         <  2:1232541/135‑2 (MQ=255)
                                 ggcAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTg                                                                                                                      >  2:240455/1‑150 (MQ=255)
                                  gcAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTgg                                                                                                                     <  2:1102918/150‑1 (MQ=255)
                                             aGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTc                                                                                                          >  1:62009/1‑150 (MQ=255)
                                             aGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTc                                                                                                          <  2:785978/150‑1 (MQ=255)
                                                        cgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACgg                                                                                               >  1:190064/1‑150 (MQ=255)
                                                             cccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                             >  1:1325661/1‑99 (MQ=255)
                                                             cccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                             <  2:1325661/99‑1 (MQ=255)
                                                              ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                               >  2:1340429/1‑112 (MQ=255)
                                                              ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                               <  1:1340429/112‑1 (MQ=255)
                                                               cAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAg                                                                                        <  2:32011/150‑1 (MQ=255)
                                                                aaCTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                       >  2:84454/1‑150 (MQ=255)
                                                                    gTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                      >  1:152664/1‑131 (MQ=255)
                                                                    gTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                      <  2:152664/131‑1 (MQ=255)
                                                                        aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACgcgc                                                                                                                                                      <  1:656346/79‑1 (MQ=255)
                                                                        aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACgcgc                                                                                                                                                      >  2:656346/1‑79 (MQ=255)
                                                                             aTGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCa                                                                          <  2:999231/150‑1 (MQ=255)
                                                                                 ccTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCAt                                                                      >  1:698884/1‑150 (MQ=255)
                                                                                  cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                       >  2:1346745/1‑132 (MQ=255)
                                                                                  cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                       <  1:1346745/132‑1 (MQ=255)
                                                                                  cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATc                                                                     >  2:1424310/1‑150 (MQ=255)
                                                                                  cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCACTTCGAATTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGTCCTCGGTTTCGGTCTGCACTGCGATCAGGCTCATCTCGGCCAGCATc                                                                     <  2:472861/150‑1 (MQ=255)
                                                                                     gcgcgTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGc                                                                  >  1:616918/1‑150 (MQ=255)
                                                                                         gTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCggg                                                              >  1:1356385/1‑150 (MQ=255)
                                                                                                   cTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                    >  1:310950/1‑150 (MQ=255)
                                                                                                      ttCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGTACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCaaa                                                 >  2:1181545/1‑150 (MQ=255)
                                                                                                      ttCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCaaa                                                 >  1:777512/1‑150 (MQ=255)
                                                                                                          tcgATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                             <  2:1356385/150‑1 (MQ=255)
                                                                                                          tcgATGGTCAGGTCGAACTTGATCAAGCCGGTGACTTGGACGCGCTCAGGGCGGGCAGCCAGGTCACAAAGGCACTGGGCCGCGGCATCCGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGCGTCAATGTGGCAAAgcgc                                             >  1:1169815/1‑150 (MQ=255)
                                                                                                             aTGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCa                                          >  2:1037396/1‑150 (MQ=255)
                                                                                                               ggTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATa                                        >  1:1295353/1‑150 (MQ=255)
                                                                                                                gTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAg                                       <  2:1295353/150‑1 (MQ=255)
                                                                                                                        gAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGc                               >  1:535417/1‑150 (MQ=255)
                                                                                                                           cTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGa                            <  2:777512/150‑1 (MQ=255)
                                                                                                                           cTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGa                            <  1:1181545/150‑1 (MQ=255)
                                                                                                                            ttGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGGTCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCgcg                           >  2:160291/1‑148 (MQ=255)
                                                                                                                              gATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAgcg                         >  2:1103587/1‑150 (MQ=255)
                                                                                                                                tCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCCCCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCt                       <  2:310950/150‑1 (MQ=255)
                                                                                                                                      cGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACa                 >  1:1100552/1‑150 (MQ=255)
                                                                                                                                       ggTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACaa                <  2:616918/150‑1 (MQ=255)
                                                                                                                                             gtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCgg                                                               <  2:1063954/96‑1 (MQ=255)
                                                                                                                                             gtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCgg                                                               >  1:1063954/2‑97 (MQ=255)
                                                                                                                                                 aCGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg      <  1:1103587/150‑1 (MQ=255)
                                                                                                                                                     gcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATCAGGACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCa  >  1:435985/1‑150 (MQ=255)
                                                                                                                                                     gcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGccc  >  1:849199/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATCAAGGCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCA  >  NC_002947/5608896‑5609194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑