Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,609,048 C→G 8.0% E201Q (GAG→CAG)  waaA ← 3‑deoxy‑D‑manno‑octulosonic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,609,0480CG 8.0% 154.6 / 5.1 50E201Q (GAG→CAG) waaA3‑deoxy‑D‑manno‑octulosonic acid transferase
Reads supporting (aligned to +/- strand):  ref base C (28/18);  new base G (2/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAGC  >  NC_002947/5608906‑5609196
                                                                                                                                              |                                                                                                                                                    
tCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                               <  1:1272037/150‑1 (MQ=255)
             gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCAc                                                                                                                                  <  2:892125/150‑1 (MQ=255)
             gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCAc                                                                                                                                  >  1:1296011/1‑150 (MQ=255)
              ggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                                 >  1:545738/1‑150 (MQ=255)
                   tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                           >  1:1232541/1‑134 (MQ=255)
                   tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                           <  2:1232541/135‑2 (MQ=255)
                       ggcAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTg                                                                                                                        >  2:240455/1‑150 (MQ=255)
                        gcAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTgg                                                                                                                       <  2:1102918/150‑1 (MQ=255)
                                   aGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTc                                                                                                            >  1:62009/1‑150 (MQ=255)
                                   aGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTc                                                                                                            <  2:785978/150‑1 (MQ=255)
                                              cgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACgg                                                                                                 >  1:190064/1‑150 (MQ=255)
                                                   cccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                               <  2:1325661/99‑1 (MQ=255)
                                                   cccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                               >  1:1325661/1‑99 (MQ=255)
                                                    ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                                 <  1:1340429/112‑1 (MQ=255)
                                                    ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                                 >  2:1340429/1‑112 (MQ=255)
                                                     cAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAg                                                                                          <  2:32011/150‑1 (MQ=255)
                                                      aaCTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                         >  2:84454/1‑150 (MQ=255)
                                                          gTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                        <  2:152664/131‑1 (MQ=255)
                                                          gTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                        >  1:152664/1‑131 (MQ=255)
                                                                   aTGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCa                                                                            <  2:999231/150‑1 (MQ=255)
                                                                       ccTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCAt                                                                        >  1:698884/1‑150 (MQ=255)
                                                                        cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                         >  2:1346745/1‑132 (MQ=255)
                                                                        cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAgg                                                                                         <  1:1346745/132‑1 (MQ=255)
                                                                        cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATc                                                                       >  2:1424310/1‑150 (MQ=255)
                                                                        cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCACTTCGAATTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGTCCTCGGTTTCGGTCTGCACTGCGATCAGGCTCATCTCGGCCAGCATc                                                                       <  2:472861/150‑1 (MQ=255)
                                                                           gcgcgTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGc                                                                    >  1:616918/1‑150 (MQ=255)
                                                                               gTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCggg                                                                >  1:1356385/1‑150 (MQ=255)
                                                                                         cTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                      >  1:310950/1‑150 (MQ=255)
                                                                                            ttCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGTACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCaaa                                                   >  2:1181545/1‑150 (MQ=255)
                                                                                            ttCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCaaa                                                   >  1:777512/1‑150 (MQ=255)
                                                                                                tcgATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                               <  2:1356385/150‑1 (MQ=255)
                                                                                                tcgATGGTCAGGTCGAACTTGATCAAGCCGGTGACTTGGACGCGCTCAGGGCGGGCAGCCAGGTCACAAAGGCACTGGGCCGCGGCATCCGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGCGTCAATGTGGCAAAgcgc                                               >  1:1169815/1‑150 (MQ=255)
                                                                                                   aTGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCa                                            >  2:1037396/1‑150 (MQ=255)
                                                                                                     ggTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATa                                          >  1:1295353/1‑150 (MQ=255)
                                                                                                      gTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAg                                         <  2:1295353/150‑1 (MQ=255)
                                                                                                              gAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGc                                 >  1:535417/1‑150 (MQ=255)
                                                                                                                 cTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGa                              <  1:1181545/150‑1 (MQ=255)
                                                                                                                 cTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGa                              <  2:777512/150‑1 (MQ=255)
                                                                                                                  ttGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGGTCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCgcg                             >  2:160291/1‑148 (MQ=255)
                                                                                                                    gATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAgcg                           >  2:1103587/1‑150 (MQ=255)
                                                                                                                      tCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCCCCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCt                         <  2:310950/150‑1 (MQ=255)
                                                                                                                            cGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACa                   >  1:1100552/1‑150 (MQ=255)
                                                                                                                             ggTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACaa                  <  2:616918/150‑1 (MQ=255)
                                                                                                                                   gtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCgg                                                                 >  1:1063954/2‑97 (MQ=255)
                                                                                                                                   gtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCgg                                                                 <  2:1063954/96‑1 (MQ=255)
                                                                                                                                       aCGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg        <  1:1103587/150‑1 (MQ=255)
                                                                                                                                           gcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATCAGGACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCa    >  1:435985/1‑150 (MQ=255)
                                                                                                                                           gcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGccc    >  1:849199/1‑149 (MQ=255)
                                                                                                                                             tCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGTCGAGCGCTCAGACAAGCGCGCATTGGCCAgc  >  1:1373524/1‑150 (MQ=255)
                                                                                                                                             tCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAgc  >  2:498511/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAGC  >  NC_002947/5608906‑5609196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑