Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,648,260 G→C 15.0% intergenic (+16/+8) elaA → / ← PP_4957 acetyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,648,2600GC15.0% 97.9 / 9.5 39intergenic (+16/+8)elaA/PP_4957acetyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (14/19);  new base C (2/4);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GTTGGCCGGGGGTGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTCCTGCTGTTCGAACTCGCAGACGATGT  >  NC_002947/5648111‑5648407
                                                                                                                                                     |                                                                                                                                                   
gttgGCCGGGGGTGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCggg                                                                                                                                                     <  1:1353490/150‑1 (MQ=255)
         gggTGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacagg                                                                                                                                            <  1:586035/150‑3 (MQ=255)
            tGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAgg                                                                                                                                         <  1:1286383/150‑1 (MQ=255)
            tGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAgg                                                                                                                                         <  1:149683/150‑1 (MQ=255)
            tGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacagggtg                                                                                                                                         <  1:170441/150‑6 (MQ=255)
             gCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAggg                                                                                                                                        <  1:677411/150‑1 (MQ=255)
                             gCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTcg                                                                                                                        >  1:647457/1‑150 (MQ=255)
                              cTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGCATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTcgc                                                                                                                       <  2:291204/150‑1 (MQ=255)
                                       aTTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacagggtg                                                                                                                                         <  1:1301867/123‑6 (MQ=255)
                                       aTTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacagggtg                                                                                                                                         >  2:1301867/1‑118 (MQ=255)
                                             aGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGt                                                                                                        >  2:1333006/1‑150 (MQ=255)
                                               gCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacaggg                                                                                                                                           >  2:951003/1‑110 (MQ=255)
                                               gCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGCCCGcacaggg                                                                                                                                           <  1:951003/113‑4 (MQ=255)
                                                   tttATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCTACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGga                                                                                                  <  1:446572/150‑1 (MQ=255)
                                                   tttATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCAACCTGTGGGGGCCGCCCCAGGGGTGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGga                                                                                                  <  2:49802/150‑1 (MQ=255)
                                                      aTGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTcgc                                                                                                                       >  1:19286/1‑126 (MQ=255)
                                                      aTGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTcgc                                                                                                                       <  2:19286/126‑1 (MQ=255)
                                                          ccGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCa                                                                                           <  1:65755/150‑1 (MQ=255)
                                                                             gtggGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGGGGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGccccc                                                                        >  1:1170247/1‑150 (MQ=255)
                                                                             gtggGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCNCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGccccc                                                                        <  1:1039515/150‑1 (MQ=255)
                                                                                  cGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTc                                                                   >  2:893761/1‑150 (MQ=255)
                                                                                     ggagTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATg                                                                <  2:450654/150‑1 (MQ=255)
                                                                                         tttttGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATg                                                            <  2:336053/150‑1 (MQ=255)
                                                                                            ttGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCACCAGGTCGGGGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGa                                                         <  2:749741/150‑1 (MQ=255)
                                                                                               gaggaCGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATaa                                                      <  1:1333006/150‑1 (MQ=255)
                                                                                               gaggaCGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATaa                                                      >  1:378959/1‑150 (MQ=255)
                                                                                                      gCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGTTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCgg                                               >  2:126275/1‑150 (MQ=255)
                                                                                                         tCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGAt                                                                               <  1:862909/115‑1 (MQ=255)
                                                                                                         tCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGAt                                                                               >  2:862909/1‑115 (MQ=255)
                                                                                                            cgTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGTTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGt                                         <  1:126275/150‑1 (MQ=255)
                                                                                                             gTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCt                                                                                      >  2:1335433/1‑104 (MQ=255)
                                                                                                             gTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCt                                                                                      <  1:1335433/104‑1 (MQ=255)
                                                                                                              tCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTtg                                       >  2:1272197/1‑150 (MQ=255)
                                                                                                                 aTTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCCCAACAGGGGTAACCCAGCACTTCGCGGATCGGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTtgcgt                                    >  1:388191/1‑150 (MQ=255)
                                                                                                                             aaGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTCctgc                        >  2:202497/1‑150 (MQ=255)
                                                                                                                                  aTGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTCCTGCTGTTc                   <  2:412914/150‑1 (MQ=255)
                                                                                                                                       cccACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTc                            >  1:660888/1‑136 (MQ=255)
                                                                                                                                       cccACCCTGTGCGGGCCGCACAGCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTc                            <  2:660888/136‑1 (MQ=255)
                                                                                                                                        ccACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGt                                         >  1:69181/1‑122 (MQ=255)
                                                                                                                                        ccACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGt                                         <  2:69181/122‑1 (MQ=255)
                                                                                                                                                   gggCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTCCTGCTGTTCGAACTCGCAGACGATGt  >  1:640493/1‑150 (MQ=255)
                                                                                                                                                    ggCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATg                                                            <  2:493525/91‑1 (MQ=255)
                                                                                                                                                    ggCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATg                                                            >  1:493525/1‑91 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTTGGCCGGGGGTGCCGGTTTATCTGTCGGCTCCGGCGCATTTACAGGCGTTTTATGCCCGCCATGGGTTTGCGGTGGTGGGCGAGGAGTTTTTGGAGGACGGCATCGCGTCCATTGGAATGCGCAAGGGATGAGCCCACCCTGTGCGGGCCGCACATCAGGGGTAACCCAGCACTTCGCGGATCTGCCGCAAGTGACGGATGATCCACTGCTTGTCGATCGCCCCCCAGTCATGAATGCGATAATGCCCGGCGTGGTTGCGTGCGCCTTCCTGCTGTTCGAACTCGCAGACGATGT  >  NC_002947/5648111‑5648407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑