Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,697,372 G→C 5.7% intergenic (+26/‑51) hslV → / → hslU ATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,697,3720GC 5.7% 212.4 / 4.4 70intergenic (+26/‑51)hslV/hslUATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit
Reads supporting (aligned to +/- strand):  ref base G (38/28);  new base C (2/2);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

GAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCA  >  NC_002947/5697229‑5697516
                                                                                                                                               |                                                                                                                                                
gAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAg                                                                                                                                            <  1:880252/150‑1 (MQ=255)
   cAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAgggg                                                                                                                                         >  2:4174/1‑150 (MQ=255)
    aaGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGc                                                                                                                                        >  2:67943/1‑150 (MQ=255)
    aaGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAGGGGc                                                                                                                                        <  2:325945/150‑1 (MQ=255)
      gaccgaccTCTCGGCCCGCGAAATCTCCGAAACCGACCTGAACATCGCCGGCGACATCTGCGTGTTCAACAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCtt                                                                                                                                      >  2:242005/1‑150 (MQ=255)
       accgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCttt                                                                                                                                     <  2:1309557/150‑1 (MQ=255)
        ccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGt                                                                                                                                                <  1:839868/138‑1 (MQ=255)
        ccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGt                                                                                                                                                >  2:839868/1‑138 (MQ=255)
        ccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTCCTGGCGCCCCTTCACCGGGAAGGGGcgcca                                                                                                                                    <  2:1215592/150‑5 (MQ=255)
         cgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTc                                                                                                                                   <  2:961661/150‑1 (MQ=255)
         cgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTc                                                                                                                                   >  2:23195/1‑150 (MQ=255)
         cgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCAGACTGATTCAGCTCTGCTGGCGCCCCTTCCCGGTGAACGGGCTTTTc                                                                                                                                   >  2:663108/1‑150 (MQ=255)
            ccTTTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATTTGCGTGTTCACCAACCACAACCTGTCCATTGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCTCGGTGAAGGTGTTTTTCCAc                                                                                                                                <  2:635552/150‑1 (MQ=255)
              tctcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGCAGGGGCTTTTCCACGc                                                                                                                              >  1:554750/1‑150 (MQ=255)
                             tCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTTCTGCACCCCTTCCCGGTGAAGGGGCTTTTCCACACTGCCTCACCTTCCCa                                                                                                               >  1:1223873/1‑150 (MQ=255)
                             tCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTATTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCa                                                                                                               >  1:1357828/1‑150 (MQ=255)
                              cGCCGAGACCGCCCTGAACATCGCCGGCGACCTCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCaa                                                                                                              <  1:803994/150‑1 (MQ=255)
                               gCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAg                                                                                                             >  1:1252435/1‑150 (MQ=255)
                                ccGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAgggg                                                                                                                                         >  1:353366/1‑121 (MQ=255)
                                ccGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAgggg                                                                                                                                         <  2:353366/121‑1 (MQ=255)
                                         cccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTg                                                                                                   >  2:1226294/1‑150 (MQ=255)
                                          ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGa                                                                                                  >  2:868512/1‑150 (MQ=255)
                                          ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGa                                                                                                  >  1:1305055/1‑150 (MQ=255)
                                                 aTCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGCCTCACCTGCCCAAGGACCGTATTGATCATGTc                                                                                           >  1:181781/1‑150 (MQ=255)
                                                 aTCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTc                                                                                           <  2:1043425/150‑1 (MQ=255)
                                                 aTCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGGGAAGGGGCTTTTTCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTc                                                                                           >  1:627561/1‑150 (MQ=255)
                                                    gCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCAt                                                                                        <  2:364152/150‑1 (MQ=255)
                                                      cGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGa                                                                                      >  2:669115/1‑150 (MQ=255)
                                                        gCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcc                                                                                    <  2:1132879/150‑1 (MQ=255)
                                                          gACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGACGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcccc                                                                                  >  2:691990/1‑150 (MQ=255)
                                                          gACATCTGCGTGTTCACCAACCACAACCTGACCATAGAGTAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcccc                                                                                  >  1:114304/1‑150 (MQ=255)
                                                              tCTTCGTGTTCACCAACCAGAATCTCACAATAGAGGAGTAGCACCTGGCCGACTGATTCTGCTGTTCTGGCGCCCCGTCCCGGTGAAGGGGCTTTTCCACGCTGACTCCCCTGCCCAAGCATAGTATTGATCATGTCCATGTCTCCCcgc                                                                              <  2:182807/150‑1 (MQ=255)
                                                               cTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgcg                                                                             >  1:1183400/1‑150 (MQ=255)
                                                                tGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCga                                                                            >  1:613870/1‑150 (MQ=255)
                                                                   gtgtTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCgagag                                                                         >  1:619647/1‑149 (MQ=255)
                                                                   gtgtTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGAt                                                                         >  1:989915/1‑150 (MQ=255)
                                                                    tgtTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATc                                                                        <  1:66038/150‑1 (MQ=255)
                                                                       tCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTc                                                                     <  1:308097/150‑1 (MQ=255)
                                                                         accaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCa                                                                   >  2:948298/1‑150 (MQ=255)
                                                                          ccaaccaCAACCAGCCCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTTTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCAc                                                                  <  1:428044/150‑1 (MQ=255)
                                                                           caaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACg                                                                 >  2:377342/1‑150 (MQ=255)
                                                                             accaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGaa                                                               <  1:242005/150‑1 (MQ=255)
                                                                                    ccTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAcc                                                        >  2:662952/1‑150 (MQ=255)
                                                                                    ccTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAcc                                                        <  1:662952/150‑1 (MQ=255)
                                                                                    ccTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAcc                                                        <  2:265951/150‑1 (MQ=255)
                                                                                    ccTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTCTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAcc                                                        >  2:1190105/1‑150 (MQ=255)
                                                                                      tGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGc                                                      <  1:669115/150‑1 (MQ=255)
                                                                                          cATCGAGGCGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAc                                                         <  2:991650/143‑1 (MQ=255)
                                                                                          cATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAAc                                                         >  1:991650/1‑143 (MQ=255)
                                                                                          cATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCcaca                                                  >  1:855275/1‑150 (MQ=255)
                                                                                          cATCGAGGAGAAGGACCTGGCCGACTGATTAAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCcaca                                                  >  2:1086026/1‑150 (MQ=255)
                                                                                                ggagAAGGACCTGGACGACTGATTCAGCTGTTCTGGCGCTCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCg                                            <  1:8080/150‑1 (MQ=255)
                                                                                                  agCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGc                                          <  2:1357828/150‑1 (MQ=255)
                                                                                                              ccGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAAc                                                              >  2:18213/1‑118 (MQ=255)
                                                                                                              ccGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAAc                                                              <  1:18213/118‑1 (MQ=255)
                                                                                                                gACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCgag                                                                           >  2:1326392/1‑103 (MQ=255)
                                                                                                                gACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCgag                                                                           <  1:1326392/103‑1 (MQ=255)
                                                                                                                          gCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGga                                     <  2:121248/131‑1 (MQ=255)
                                                                                                                          gCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGga                                     >  1:121248/1‑131 (MQ=255)
                                                                                                                             gTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCAcatcat                                              >  1:877609/1‑119 (MQ=255)
                                                                                                                             gTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCCAGATCGTCCACGAACTCAACCGCCAcatcat                                              <  2:877609/119‑1 (MQ=255)
                                                                                                                               tCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCAt             <  1:868512/150‑1 (MQ=255)
                                                                                                                                cTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCAtt            >  1:1235677/1‑150 (MQ=255)
                                                                                                                                 tGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTg           >  1:773653/1‑150 (MQ=255)
                                                                                                                                 tGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTg           >  1:176037/1‑150 (MQ=255)
                                                                                                                                  aaagcCCCTTCACCGGGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTAGACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGc          >  1:1248334/4‑150 (MQ=255)
                                                                                                                                      ccccTTCACCGGGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTg      >  1:1036063/1‑150 (MQ=255)
                                                                                                                                       cccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGCCCCCAAGCGCGCCGTGGCCATTGCCCTgc     <  1:691990/150‑1 (MQ=255)
                                                                                                                                       cccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc     <  1:1226294/150‑1 (MQ=255)
                                                                                                                                        ccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgcg    >  1:922894/1‑150 (MQ=255)
                                                                                                                                          ttacaggTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCa  >  2:1421802/6‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GAACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCA  >  NC_002947/5697229‑5697516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑