Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,797,368 G→T 9.4% R196R (CGC→CGA)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,797,3680GT 9.4% 230.4 / 9.8 74R196R (CGC→CGA) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (28/39);  new base T (2/5);  total (30/44)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TGTTGATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCACCGC  >  NC_002947/5797220‑5797516
                                                                                                                                                    |                                                                                                                                                    
tgtTGATCTCGCTGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgc                                                                                                                                                     >  1:413807/1‑150 (MQ=255)
 gtTGATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgcg                                                                                                                                                    >  1:136283/1‑150 (MQ=255)
  ttGATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgcgc                                                                                                                                                   >  2:482048/1‑150 (MQ=255)
   tGATCTCGCTGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgcgcg                                                                                                                                                  <  2:413807/150‑1 (MQ=255)
    gATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGTCGCGAGATCGCGCgg                                                                                                                                                 >  2:989633/1‑150 (MQ=255)
       ctcGCCGTTGTGGGTGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgt                                                                                                                                              <  2:310735/150‑3 (MQ=255)
       ctcGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgt                                                                                                                                              <  1:539317/150‑3 (MQ=255)
       ctcGCCGCTGTGGGCGAGGAATCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgt                                                                                                                                              <  2:615962/150‑3 (MQ=255)
        tcGGCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgtt                                                                                                                                             <  2:152671/150‑4 (MQ=255)
        tcGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatc                                                                                                                                             >  2:1179266/1‑150 (MQ=255)
          gCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                           <  2:380348/150‑1 (MQ=255)
          gCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                           >  1:328583/1‑150 (MQ=255)
          gCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                           >  2:550212/1‑150 (MQ=255)
               tgtgGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGc                                                                                                                                      <  2:1326395/150‑1 (MQ=255)
                gtgGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTTGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGcc                                                                                                                                     <  1:374274/150‑1 (MQ=255)
                gtgGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGcc                                                                                                                                     >  2:1275362/1‑150 (MQ=255)
                        ggAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAga                                                                                                                             <  1:947376/150‑1 (MQ=255)
                        ggAAGCGGAACGGCTGCGCCACCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCAGCATCAGGCCTTTGTAga                                                                                                                             <  2:731183/150‑1 (MQ=255)
                             cGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGatgatg                                                                                                                        <  2:1423291/150‑1 (MQ=255)
                               gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgt                                                                                                                                              >  1:191008/1‑124 (MQ=255)
                               gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGCGATCTCGCGGcgt                                                                                                                                              <  2:191008/126‑3 (MQ=255)
                                aaCGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTc                                                                                                                     <  2:1080750/150‑1 (MQ=255)
                                aaCGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTc                                                                                                                     >  2:948577/1‑150 (MQ=255)
                                    gCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                 >  2:1092355/1‑150 (MQ=255)
                                    gCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                 <  2:328583/150‑1 (MQ=255)
                                    gCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                 <  1:806509/150‑1 (MQ=255)
                                    gCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                 <  1:1075650/150‑1 (MQ=255)
                                    gCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                 <  1:1092355/150‑1 (MQ=255)
                                      tGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGc                                                                                                               <  1:1275834/150‑1 (MQ=255)
                                            aGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAg                                                                                                         >  1:1173673/1‑150 (MQ=255)
                                            aGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAg                                                                                                         <  2:428246/150‑1 (MQ=255)
                                            aGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAg                                                                                                         <  1:989633/150‑1 (MQ=255)
                                              cGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                           <  1:606605/114‑1 (MQ=255)
                                              cGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                           >  2:606605/1‑114 (MQ=255)
                                              cGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCt                                                                                                       >  2:304450/1‑150 (MQ=255)
                                                   aTTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGa                                                                                                  >  1:1305283/1‑150 (MQ=255)
                                                         gtagtgtgtTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGTTCGCGCGGCATCATCATGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGt                                                                                            <  1:555303/145‑1 (MQ=255)
                                                         gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGt                                                                                            <  1:550212/150‑1 (MQ=255)
                                                         gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGt                                                                                            >  1:1219014/1‑150 (MQ=255)
                                                           aGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTgg                                                                                          <  1:333723/150‑1 (MQ=255)
                                                             ggTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTc                                                                                        <  1:482048/150‑1 (MQ=255)
                                                                gtTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGc                                                                                     >  2:429119/1‑150 (MQ=255)
                                                                 ttGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcg                                                                                    <  1:1108864/150‑1 (MQ=255)
                                                                       gagaAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTgg                                                                              <  2:717627/150‑1 (MQ=255)
                                                                            gcgcTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGtt                                                                         <  2:108465/150‑1 (MQ=255)
                                                                             cgcTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGCAGGTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTg                                                                        <  1:275096/150‑1 (MQ=255)
                                                                             cgcTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTg                                                                        <  1:304450/150‑1 (MQ=255)
                                                                                tggtggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAg                                                                                                   <  2:232024/120‑1 (MQ=255)
                                                                                tggtggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAg                                                                                                   >  1:232024/1‑120 (MQ=255)
                                                                                     gAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACcga                                                                >  1:1080639/1‑150 (MQ=255)
                                                                                         acGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGt                                                                                         >  2:827316/1‑121 (MQ=255)
                                                                                         acGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGt                                                                                         <  1:827316/121‑1 (MQ=255)
                                                                                           gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaacg                                                          >  2:1334600/1‑150 (MQ=255)
                                                                                                tCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgacgac                                                     <  1:429119/150‑1 (MQ=255)
                                                                                                tCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgacgac                                                     <  1:1179266/150‑1 (MQ=255)
                                                                                                  gcgGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACgg                                                   <  2:136283/150‑1 (MQ=255)
                                                                                                        tGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTg                                             <  2:61444/150‑1 (MQ=255)
                                                                                                           aGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCGGGGCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGACGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAAc                                          >  1:591225/1‑150 (MQ=255)
                                                                                                             gcgcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAg                                        >  1:1367022/1‑150 (MQ=255)
                                                                                                             gcgcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAg                                        >  1:914787/1‑150 (MQ=255)
                                                                                                               gcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTg                                                                                                      >  1:25092/1‑86 (MQ=255)
                                                                                                               gcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTg                                                                                                      <  2:25092/86‑1 (MQ=255)
                                                                                                               gcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCt                                      <  1:948577/150‑1 (MQ=255)
                                                                                                                   gTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGGCACCGACGAACGACGACGGGCACTGAACAGCTTGAt                                  >  2:643664/1‑150 (MQ=255)
                                                                                                                    tCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTg                                                                                                                                 <  1:499355/54‑1 (MQ=255)
                                                                                                                    tCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTg                                                                                                                                 >  2:499355/1‑54 (MQ=255)
                                                                                                                        ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTg                                                                               <  2:1050898/100‑1 (MQ=255)
                                                                                                                        ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTg                                                                               >  1:1050898/1‑100 (MQ=255)
                                                                                                                        ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACgg                                                   >  1:252928/1‑128 (MQ=255)
                                                                                                                        ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACgg                                                   <  2:252928/128‑1 (MQ=255)
                                                                                                                             tCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTcc                        >  1:33117/1‑150 (MQ=255)
                                                                                                                                ggATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTgg                     <  2:914787/150‑1 (MQ=255)
                                                                                                                                      aaCGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTc               >  1:1257314/1‑150 (MQ=255)
                                                                                                                                        tgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAg             >  2:642439/2‑150 (MQ=255)
                                                                                                                                         gccgcGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAgg            <  2:1080639/150‑1 (MQ=255)
                                                                                                                                             cgAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGAt                                                                                                 >  2:606783/1‑61 (MQ=255)
                                                                                                                                             cgAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGAt                                                                                                 <  1:606783/61‑1 (MQ=255)
                                                                                                                                              gagaTCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTc       <  2:1219014/150‑1 (MQ=255)
                                                                                                                                                   cgcgcgGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCACCGc  <  2:33117/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTTGATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCACCGC  >  NC_002947/5797220‑5797516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑