Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,897,995 G→C 7.0% Y983* (TAC→TAG)  PP_5173 ← RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,897,9950GC 7.0% 220.0 / 6.5 71Y983* (TAC→TAG) PP_5173RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base C (2/3);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

CATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTC  >  NC_002947/5897847‑5898141
                                                                                                                                                    |                                                                                                                                                  
cATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGt                                                                                                                                                   >  1:1338931/1‑150 (MQ=255)
 aTTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTa                                                                                                                                                  <  2:920737/150‑1 (MQ=255)
  ttCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAg                                                                                                                                                 >  1:169376/1‑150 (MQ=255)
         gTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCTGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATga                                                                                                                                          <  1:764495/150‑2 (MQ=255)
            aaCCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattg                                                                                                                                       <  2:273208/150‑5 (MQ=255)
              cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                     >  1:4485/1‑150 (MQ=255)
              cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                     >  2:215189/1‑150 (MQ=255)
               ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcccc                                                                                                                                    <  2:998288/150‑1 (MQ=255)
               ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCATCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcccc                                                                                                                                    >  1:727829/1‑150 (MQ=255)
                cGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCa                                                                                                                                   <  2:706439/150‑1 (MQ=255)
                   tCCTACCAGGGGCCATATGGGCCTTCAGGCGTGCTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCTGTCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                                <  1:1108484/150‑1 (MQ=255)
                     cTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAaca                                                                                                                              >  1:1368367/1‑150 (MQ=255)
                      tACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAacac                                                                                                                             >  2:883045/1‑150 (MQ=255)
                         cAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:1261058/1‑150 (MQ=255)
                         cAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:1311713/1‑150 (MQ=255)
                         cAGGGGCCATATGGGCCTTCAGGCGTGTTTCGGCGGCTCGCGGATCTTGTACAAGGCGACGTACAGCGCCGGCTGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:531830/1‑150 (MQ=255)
                          aGGGGCCATATGGTCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTcg                                                                                                                         <  1:588765/150‑1 (MQ=255)
                             ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGt                                                                                                                      <  1:631098/150‑1 (MQ=255)
                             ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattggc                                                                                                                                     <  2:1093623/135‑7 (MQ=255)
                             ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattggc                                                                                                                                     >  1:1093623/1‑129 (MQ=255)
                               ccATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGAGCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCCCGCGTGc                                                                                                                    >  2:821625/1‑150 (MQ=255)
                                cATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGc                                                                                                                                       <  1:28343/130‑1 (MQ=255)
                                cATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGc                                                                                                                                       >  2:28343/1‑130 (MQ=255)
                                      ggCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCggg                                                                                                             >  1:144020/1‑150 (MQ=255)
                                        ccTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgggg                                                                                                                                        <  2:1384509/121‑1 (MQ=255)
                                        ccTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgggg                                                                                                                                        >  1:1384509/1‑121 (MQ=255)
                                          ttCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACCGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCa                                                                                                         >  2:8593/1‑150 (MQ=255)
                                                    tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCt                                                                                               >  1:1014269/1‑150 (MQ=255)
                                                    tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCt                                                                                               >  1:353287/1‑150 (MQ=255)
                                                        tGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCGAGGGCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgcc                                                                                           <  2:42691/150‑1 (MQ=255)
                                                             cTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgc                                                                                      <  1:558079/150‑1 (MQ=255)
                                                              tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgcc                                                                                     >  1:410874/1‑150 (MQ=255)
                                                                     tCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTca                                                                              <  2:1338931/150‑1 (MQ=255)
                                                                        tGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCCGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagca                                                                           <  2:760901/150‑1 (MQ=255)
                                                                        tGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagca                                                                           <  2:410874/150‑1 (MQ=255)
                                                                         gTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAGGGGTTTTCATGCCCATGCGCGCCGCCGCCGCGGTcagcag                                                                          >  2:1084607/1‑150 (MQ=255)
                                                                           aCCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGa                                                                        <  2:531830/150‑1 (MQ=255)
                                                                              aGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcg                                                                     >  2:1368698/1‑150 (MQ=255)
                                                                                gCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggc                                                                   <  2:186933/150‑1 (MQ=255)
                                                                                  catggaCAGCGCCGGCAGAAACAGCCGTATCGGTAAGGTCGCCACGTTGATCCCGCCAATCATGGTGTGGGCCATTTTCCCCCATAAGGCCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGAGcggccg                                                                 <  2:247298/145‑1 (MQ=255)
                                                                                           cgcCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCggtg                                                        <  2:1311713/150‑1 (MQ=255)
                                                                                              cGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     >  1:128065/1‑150 (MQ=255)
                                                                                              cGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     <  2:144020/150‑1 (MQ=255)
                                                                                                gCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttgg                                                   >  2:481658/1‑150 (MQ=255)
                                                                                                gCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttgg                                                   <  1:215189/150‑1 (MQ=255)
                                                                                                  aGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTtggtg                                                 <  2:169376/150‑1 (MQ=255)
                                                                                                   ggAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTtggtgg                                                >  1:641153/1‑150 (MQ=255)
                                                                                                    gAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGc                                               >  2:664257/1‑150 (MQ=255)
                                                                                                      aCAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCt                                             <  2:353287/150‑1 (MQ=255)
                                                                                                        agcagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTcc                                           >  1:634733/1‑150 (MQ=255)
                                                                                                          cagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTccac                                         <  1:8593/150‑1 (MQ=255)
                                                                                                              gTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCCCGGTCTGCAGCAGCTTCCCGCCTCGGGGGTTTGTGGCCTCCaccacc                                     >  2:447917/1‑150 (MQ=255)
                                                                                                                 aGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTg                                                                                                                     >  1:106618/1‑67 (MQ=255)
                                                                                                                 aGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTg                                                                                                                     <  2:106618/67‑1 (MQ=255)
                                                                                                                        tCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACg                           <  2:1330702/150‑1 (MQ=255)
                                                                                                                           ccACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGACCCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCCCGGCg                        >  2:623564/1‑150 (MQ=255)
                                                                                                                            cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccg                                                                                          <  1:1228542/83‑1 (MQ=255)
                                                                                                                            cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccg                                                                                          >  2:1228542/1‑83 (MQ=255)
                                                                                                                            cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     >  2:499866/1‑120 (MQ=255)
                                                                                                                            cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     <  1:499866/120‑1 (MQ=255)
                                                                                                                                   atCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCCCCCCCGGATGACAGGGCGAGAAGccc                >  2:1150563/1‑150 (MQ=255)
                                                                                                                                     cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATc                                                                                                                >  2:999338/1‑52 (MQ=255)
                                                                                                                                     cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATc                                                                                                                <  1:999338/52‑1 (MQ=255)
                                                                                                                                     cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTg              <  1:1084607/150‑1 (MQ=255)
                                                                                                                                      ccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATTATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTgg             <  2:1261058/150‑1 (MQ=255)
                                                                                                                                      ccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTgg             >  1:290159/1‑150 (MQ=255)
                                                                                                                                           aaTCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTc        <  2:641153/150‑1 (MQ=255)
                                                                                                                                             ccATGGCCTACGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGTCTCCACCACCGCATCCCACGGCAACAAGCCCTGGGCTTCGa      >  2:214535/2‑150 (MQ=255)
                                                                                                                                                tGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGAACGACGCCGCCGCGGTCAGCATGAGCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACt   >  1:530938/1‑150 (MQ=255)
                                                                                                                                                 ggCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTc  <  1:985141/150‑1 (MQ=255)
                                                                                                                                                 ggCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTc  <  1:664257/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTC  >  NC_002947/5897847‑5898141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑