Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,897,998	G→C	7.3%	A982A (GCC→GCG)	PP_5173 ←	RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,897,998	0	G	C	7.3%	210.3 / 6.6	68	A982A (GCC→GCG)	PP_5173	RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base C (2/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTC  >  NC_002947/5897849‑5898141                                                                                                                                                      |                                                                                                                                                ttCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAg                                                                                                                                                 >  1:169376/1‑150 (MQ=255)        gTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCTGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATga                                                                                                                                          <  1:764495/150‑2 (MQ=255)           aaCCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattg                                                                                                                                       <  2:273208/150‑5 (MQ=255)             cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                     >  1:4485/1‑150 (MQ=255)             cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                     >  2:215189/1‑150 (MQ=255)              ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcccc                                                                                                                                    <  2:998288/150‑1 (MQ=255)              ccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCATCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcccc                                                                                                                                    >  1:727829/1‑150 (MQ=255)               cGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCa                                                                                                                                   <  2:706439/150‑1 (MQ=255)                  tCCTACCAGGGGCCATATGGGCCTTCAGGCGTGCTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCTGTCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                                <  1:1108484/150‑1 (MQ=255)                    cTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAaca                                                                                                                              >  1:1368367/1‑150 (MQ=255)                     tACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAacac                                                                                                                             >  2:883045/1‑150 (MQ=255)                        cAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:1261058/1‑150 (MQ=255)                        cAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:1311713/1‑150 (MQ=255)                        cAGGGGCCATATGGGCCTTCAGGCGTGTTTCGGCGGCTCGCGGATCTTGTACAAGGCGACGTACAGCGCCGGCTGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                          >  1:531830/1‑150 (MQ=255)                         aGGGGCCATATGGTCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTcg                                                                                                                         <  1:588765/150‑1 (MQ=255)                            ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGt                                                                                                                      <  1:631098/150‑1 (MQ=255)                            ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattggc                                                                                                                                     <  2:1093623/135‑7 (MQ=255)                            ggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattggc                                                                                                                                     >  1:1093623/1‑129 (MQ=255)                              ccATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGAGCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCCCGCGTGc                                                                                                                    >  2:821625/1‑150 (MQ=255)                               cATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGc                                                                                                                                       <  1:28343/130‑1 (MQ=255)                               cATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGc                                                                                                                                       >  2:28343/1‑130 (MQ=255)                                     ggCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCggg                                                                                                             >  1:144020/1‑150 (MQ=255)                                       ccTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgggg                                                                                                                                        <  2:1384509/121‑1 (MQ=255)                                       ccTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgggg                                                                                                                                        >  1:1384509/1‑121 (MQ=255)                                         ttCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACCGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCa                                                                                                         >  2:8593/1‑150 (MQ=255)                                                   tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCt                                                                                               >  1:1014269/1‑150 (MQ=255)                                                   tttCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCt                                                                                               >  1:353287/1‑150 (MQ=255)                                                       tGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCGAGGGCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgcc                                                                                           <  2:42691/150‑1 (MQ=255)                                                            cTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgc                                                                                      <  1:558079/150‑1 (MQ=255)                                                             tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccgccgcc                                                                                     >  1:410874/1‑150 (MQ=255)                                                                    tCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTca                                                                              <  2:1338931/150‑1 (MQ=255)                                                                       tGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCCGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagca                                                                           <  2:760901/150‑1 (MQ=255)                                                                       tGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagca                                                                           <  2:410874/150‑1 (MQ=255)                                                                        gTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAGGGGTTTTCATGCCCATGCGCGCCGCCGCCGCGGTcagcag                                                                          >  2:1084607/1‑150 (MQ=255)                                                                          aCCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGa                                                                        <  2:531830/150‑1 (MQ=255)                                                                             aGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcg                                                                     >  2:1368698/1‑150 (MQ=255)                                                                               gCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggc                                                                   <  2:186933/150‑1 (MQ=255)                                                                                 catggaCAGCGCCGGCAGAAACAGCCGTATCGGTAAGGTCGCCACGTTGATCCCGCCAATCATGGTGTGGGCCATTTTCCCCCATAAGGCCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGAGcggccg                                                                 <  2:247298/145‑1 (MQ=255)                                                                                          cgcCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCggtg                                                        <  2:1311713/150‑1 (MQ=255)                                                                                             cGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     <  2:144020/150‑1 (MQ=255)                                                                                             cGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     >  1:128065/1‑150 (MQ=255)                                                                                               gCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttgg                                                   <  1:215189/150‑1 (MQ=255)                                                                                               gCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttgg                                                   >  2:481658/1‑150 (MQ=255)                                                                                                 aGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTtggtg                                                 <  2:169376/150‑1 (MQ=255)                                                                                                  ggAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTtggtgg                                                >  1:641153/1‑150 (MQ=255)                                                                                                   gAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGc                                               >  2:664257/1‑150 (MQ=255)                                                                                                     aCAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCt                                             <  2:353287/150‑1 (MQ=255)                                                                                                       agcagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTcc                                           >  1:634733/1‑150 (MQ=255)                                                                                                         cagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTccac                                         <  1:8593/150‑1 (MQ=255)                                                                                                             gTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCCCGGTCTGCAGCAGCTTCCCGCCTCGGGGGTTTGTGGCCTCCaccacc                                     >  2:447917/1‑150 (MQ=255)                                                                                                                aGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTg                                                                                                                     >  1:106618/1‑67 (MQ=255)                                                                                                                aGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTg                                                                                                                     <  2:106618/67‑1 (MQ=255)                                                                                                                       tCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACg                           <  2:1330702/150‑1 (MQ=255)                                                                                                                          ccACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGACCCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCCCGGCg                        >  2:623564/1‑150 (MQ=255)                                                                                                                           cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccg                                                                                          <  1:1228542/83‑1 (MQ=255)                                                                                                                           cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgccg                                                                                          >  2:1228542/1‑83 (MQ=255)                                                                                                                           cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     >  2:499866/1‑120 (MQ=255)                                                                                                                           cACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggtt                                                     <  1:499866/120‑1 (MQ=255)                                                                                                                                  atCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCCCCCCCGGATGACAGGGCGAGAAGccc                >  2:1150563/1‑150 (MQ=255)                                                                                                                                    cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATc                                                                                                                >  2:999338/1‑52 (MQ=255)                                                                                                                                    cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATc                                                                                                                <  1:999338/52‑1 (MQ=255)                                                                                                                                    cccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTg              <  1:1084607/150‑1 (MQ=255)                                                                                                                                     ccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATTATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTgg             <  2:1261058/150‑1 (MQ=255)                                                                                                                                     ccGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTgg             >  1:290159/1‑150 (MQ=255)                                                                                                                                          aaTCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTc        <  2:641153/150‑1 (MQ=255)                                                                                                                                            ccATGGCCTACGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGTCTCCACCACCGCATCCCACGGCAACAAGCCCTGGGCTTCGa      >  2:214535/2‑150 (MQ=255)                                                                                                                                               tGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGAACGACGCCGCCGCGGTCAGCATGAGCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACt   >  1:530938/1‑150 (MQ=255)                                                                                                                                                ggCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTc  <  1:985141/150‑1 (MQ=255)                                                                                                                                                ggCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTc  <  1:664257/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                TTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTC  >  NC_002947/5897849‑5898141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑