Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,914,182 C→G 13.0% intergenic (+18/+135) spuI → / ← argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,914,1820CG13.0% 145.2 / 12.9 54intergenic (+18/+135)spuI/argAglutamylpolyamine synthetase/amino acid N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base G (4/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

AGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGC  >  NC_002947/5914037‑5914326
                                                                                                                                                 |                                                                                                                                                
agaTCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAgg                                                                                                                                              <  1:717169/150‑1 (MQ=255)
   tCCTGAACAAGAACATCGAGCCGAAGTACATCGACATCTTCGGCTCGTGCGCGCACAAGGTGGGGGCAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGCTACCTGCATACCGTGTAAACGAAACCGCAGCCCCTCAAGggcg                                                                                                                                           >  2:1118983/1‑150 (MQ=255)
    ccTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcgg                                                                                                                                          <  1:874351/150‑1 (MQ=255)
       gAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGt                                                                                                                                       >  1:1074631/1‑150 (MQ=255)
         aCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGttt                                                                                                                                     >  2:1426888/1‑150 (MQ=255)
              tACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTtttgtt                                                                                                                                >  1:1092556/1‑150 (MQ=255)
                  tCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTgag                                                                                                                            >  1:921013/1‑150 (MQ=255)
                     aTCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga                                                                                                                             <  2:241808/146‑1 (MQ=255)
                     aTCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga                                                                                                                             >  1:241808/1‑146 (MQ=255)
                      tCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCg                                                                                                                        <  1:992039/150‑1 (MQ=255)
                      tCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTAGAGAACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCCAGGGCAACGTTTTTGTTTGAGACCg                                                                                                                        >  2:987389/1‑150 (MQ=38)
                        cGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGgt                                                                                                                      <  2:1092556/150‑1 (MQ=255)
                          aaGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGgtgt                                                                                                                    >  2:623176/1‑150 (MQ=255)
                           aGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTc                                                                                                                   <  1:1216792/150‑1 (MQ=255)
                               cATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGccc                                                                                                               >  1:655237/1‑150 (MQ=255)
                                aTCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCct                                                                                                              >  1:269874/1‑150 (MQ=255)
                                       tCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTcgcg                                                                                                       >  2:918035/1‑150 (MQ=255)
                                        cTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCgg                                                                                                      >  2:476402/1‑150 (MQ=255)
                                         ttCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCggg                                                                                                     <  2:1074631/150‑1 (MQ=255)
                                                      aGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTccc                                                                                        >  1:238069/1‑150 (MQ=255)
                                                       ggAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCg                                                                                       >  1:151767/1‑150 (MQ=255)
                                                           aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg                                                                                   <  1:476402/150‑1 (MQ=255)
                                                           aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg                                                                                   <  2:1348934/150‑1 (MQ=255)
                                                             cGAGCTTGAAGAGATCGAGCACTCGATCTCCGACCTCGAGTACAACTATTACCTGCATACCGTCTAAACGAAAGCGCCGCCCCTCGTGGGCGTCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGt                                                                                 <  2:238069/150‑1 (MQ=255)
                                                                 cTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAAtt                                                                             <  1:623176/150‑1 (MQ=255)
                                                                      agagTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGaa                                                                        <  2:655237/150‑1 (MQ=255)
                                                                         gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGc                                                                     >  1:854094/1‑150 (MQ=255)
                                                                            cGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGa                                                                  >  2:1062455/1‑150 (MQ=255)
                                                                             gAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGaa                                                                 <  2:854094/150‑1 (MQ=255)
                                                                               gCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGc                                                               <  1:206936/150‑1 (MQ=255)
                                                                                   tCGATCTCCGACCTCGAGTACACCTGGGCCCTGCCTGCCGTGTAAGCGATCGGGTGTCGAAGCCAGGGCGGCTTGGTGGCTGTAGATCTGTGGGTCCTGCGTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                                           >  2:872973/1‑150 (MQ=255)
                                                                                   tCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                                           <  2:269874/150‑1 (MQ=255)
                                                                                     gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGa                                                         <  2:921013/150‑1 (MQ=255)
                                                                                         tcCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTa                                                     >  2:432961/1‑150 (MQ=255)
                                                                                            gACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCt                                                  <  1:1426888/150‑1 (MQ=255)
                                                                                              ccTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt                                                <  2:151767/150‑1 (MQ=255)
                                                                                               cTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAg                                                                                    <  2:376708/113‑1 (MQ=255)
                                                                                               cTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAg                                                                                    >  1:376708/1‑113 (MQ=255)
                                                                                                tCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTgg                                              <  1:987389/150‑1 (MQ=255)
                                                                                                tCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTgg                                              <  1:432961/150‑1 (MQ=255)
                                                                                                tCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTgg                                              <  1:918035/150‑1 (MQ=255)
                                                                                                        aaCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGc                                      >  2:1293585/1‑150 (MQ=255)
                                                                                                        aaCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTGGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGc                                      <  2:902644/150‑1 (MQ=255)
                                                                                                                          gtgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGggcg                    <  1:71505/150‑1 (MQ=255)
                                                                                                                               aacaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCt                                                             <  2:1251315/100‑1 (MQ=37)
                                                                                                                               aacaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGa                                            <  2:30632/117‑1 (MQ=37)
                                                                                                                               aaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCt                                                             >  1:1251315/1‑104 (MQ=37)
                                                                                                                               aaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGa                                            >  1:30632/1‑121 (MQ=38)
                                                                                                                                acaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg                                       >  2:426525/4‑125 (MQ=39)
                                                                                                                                acaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg                                       <  1:426525/122‑1 (MQ=39)
                                                                                                                                     aaCGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTa         >  1:367011/1‑150 (MQ=255)
                                                                                                                                      aCGCCGCCCTTGAGGGGCGGCGTTTTCGTTT‑ACA‑CGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAg      >  1:978347/1‑150 (MQ=38)
                                                                                                                                          cgccCCGCAAGGGCGCCCTTTTTGTTTGAGCCCGGTGTCGCCCTCATCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTCAAGCATGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAggg    <  1:872973/150‑1 (MQ=39)
                                                                                                                                            ccccTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGGGAAGAGGGCGGCCCAGGCTTACAGGGGc  >  1:279822/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGGGC  >  NC_002947/5914037‑5914326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑