| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,962,332 | G→T | 10.1% | G391V (GGC→GTC) | lysA‑II → | diaminopimelate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,962,332 | 0 | G | T | 10.1% | 140.0 / 3.8 | 49 | G391V (GGC→GTC) | lysA‑II | diaminopimelate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (23/21); new base T (2/3); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
CTGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCA > NC_002947/5962183‑5962480 | cTGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACgg > 1:1400650/1‑150 (MQ=255) cGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGa > 2:1354434/1‑150 (MQ=255) aCTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAg < 1:550969/150‑1 (MQ=255) tGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGc < 1:868452/150‑1 (MQ=255) cGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGTCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGtt < 1:1361494/150‑1 (MQ=255) cTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGa > 1:1376492/1‑150 (MQ=255) ttCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa > 2:470173/1‑150 (MQ=255) tCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAc < 1:681781/150‑1 (MQ=255) aGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAg < 1:1338956/131‑1 (MQ=255) aGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAg > 2:1338956/1‑131 (MQ=255) ccGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa < 1:172208/136‑1 (MQ=255) ccGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa > 2:172208/1‑136 (MQ=255) cTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGATTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTc > 1:824748/1‑150 (MQ=255) ggCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACt < 1:110449/150‑1 (MQ=255) gACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGc < 1:577953/150‑1 (MQ=255) tgctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGaaa < 2:1376492/150‑1 (MQ=255) tgctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGGGCGAAGTACGCCGCCGCGGGACCGTCGCCGAACTGTATGCTGGCGaaa > 1:86725/1‑150 (MQ=255) ccGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgc > 2:206413/1‑150 (MQ=255) ccGTAGAGTCCGCGAGCGCCTATGGGTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgc > 1:392302/1‑150 (MQ=255) cGTAGAGTCCGCGGGCGCCGATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgct > 2:574779/1‑150 (MQ=255) gTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg < 1:921587/136‑1 (MQ=255) gTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg > 2:921587/1‑136 (MQ=255) tCCGCGGGCGCCTATGGTTTTGTCATGAGCCCAAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAg < 2:824748/150‑1 (MQ=255) ccGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTTCCGGAGt < 1:1354434/150‑1 (MQ=255) gcgcCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGccc < 1:712639/150‑1 (MQ=255) cTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGa < 2:369729/138‑1 (MQ=255) cTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGa > 1:369729/1‑138 (MQ=255) tATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgct > 1:1137157/1‑150 (MQ=255) tATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgct > 1:1297362/1‑150 (MQ=255) tCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCAGACATGAATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGtttt > 2:38374/1‑150 (MQ=255) cATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTa < 1:206413/150‑1 (MQ=255) cATGAGCTCCAACTACAACACCCGCGGCCGTTGAGCTGCACCCCCGGTCCCCGGGGACCAGGCGTTCGAAGGACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTa > 1:388023/1‑150 (MQ=255) tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAcc > 2:341664/1‑150 (MQ=255) tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa < 1:350719/57‑1 (MQ=255) tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa > 2:350719/1‑57 (MQ=255) cacCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg < 2:416079/150‑1 (MQ=255) ccGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGATTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCa > 2:1028045/1‑150 (MQ=255) cgcgGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc > 2:546107/1‑143 (MQ=255) cgcgGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc < 1:546107/143‑1 (MQ=255) gCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGAt > 2:472271/1‑131 (MQ=255) gCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGAt < 1:472271/131‑1 (MQ=255) gCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGAc < 2:392302/150‑1 (MQ=255) cGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACtt > 1:767164/1‑150 (MQ=255) gcgcTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATg < 1:133526/150‑1 (MQ=255) tGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTcc > 2:1237318/1‑150 (MQ=255) aaaTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg < 2:388023/150‑1 (MQ=255) cTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCAt > 2:205245/1‑138 (MQ=255) cTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCAt < 1:205245/138‑1 (MQ=255) tGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg < 2:1297362/150‑1 (MQ=255) aCGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGAGCTGGTcagc > 1:200296/1‑150 (MQ=255) ggCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTGTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcagcca < 2:1133189/150‑1 (MQ=255) | CTGCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCA > NC_002947/5962183‑5962480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |