Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,967,943 G→T 10.5% A64D (GCC→GAC)  glnK ← NRII(GlnL/NtrB) phosphatase activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,967,9430GT10.5% 165.2 / 8.4 57A64D (GCC→GAC) glnKNRII(GlnL/NtrB) phosphatase activator
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base T (3/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

TTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGC  >  NC_002947/5967795‑5968084
                                                                                                                                                    |                                                                                                                                             
ttAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGTTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGc                                                                                                                                              <  2:551101/150‑1 (MQ=255)
       gcgTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCg                                                                                                                                       >  1:1037056/1‑150 (MQ=255)
         gTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGTCGCCATCGAt                                                                                                                                     <  2:1012536/150‑1 (MQ=255)
         gTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGAt                                                                                                                                     <  2:1037056/150‑1 (MQ=255)
               aTCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCAc                                                                                                                               >  1:158053/1‑150 (MQ=255)
               aTCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCAc                                                                                                                               >  2:817643/1‑150 (MQ=255)
                   gTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGa                                                                                                                                            >  1:1063552/1‑133 (MQ=255)
                   gTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGa                                                                                                                                            <  2:1063552/133‑1 (MQ=255)
                    tttCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                          <  2:316217/150‑1 (MQ=255)
                       cGCCGGTACGGATGTGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCa                                                                                                                       <  1:771085/150‑1 (MQ=255)
                                ggATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGa                                                                                                              <  2:583638/150‑1 (MQ=255)
                                       gATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACca                                                                                                           >  2:445485/1‑146 (MQ=255)
                                       gATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACca                                                                                                           <  1:445485/146‑1 (MQ=255)
                                         tCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATAtt                                                                                                     <  2:1301458/150‑1 (MQ=255)
                                                   cAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACcgcg                                                                                           >  2:377279/1‑150 (MQ=255)
                                                          accacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGc                                                                                    >  1:178406/1‑150 (MQ=255)
                                                           ccacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCt                                                                                   >  2:575857/1‑150 (MQ=255)
                                                            cacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTc                                                                                  >  2:1172474/1‑150 (MQ=255)
                                                                  aaTCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGtgtg                                                                            <  1:377279/150‑1 (MQ=255)
                                                                   aTCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTgg                                                                           <  1:575857/150‑1 (MQ=255)
                                                                         ccGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCCTCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTc                                                                     >  1:408822/1‑150 (MQ=255)
                                                                            tCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGc                                                                  >  2:772780/1‑150 (MQ=255)
                                                                              aCCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccg                                                                >  2:590259/1‑150 (MQ=255)
                                                                                cGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccgac                                                              >  1:785792/1‑150 (MQ=255)
                                                                                   tCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccgaccga                                                           >  1:508494/1‑150 (MQ=255)
                                                                                         cGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGGGTGGCCCTTCTGCCGACCGAAACCtt                                                     >  1:300815/1‑150 (MQ=255)
                                                                                          ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  1:590259/150‑1 (MQ=255)
                                                                                          ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  1:817643/150‑1 (MQ=255)
                                                                                          ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  2:158053/150‑1 (MQ=255)
                                                                                           gtgtTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTc                                                                                                                                 >  1:865583/1‑72 (MQ=255)
                                                                                           gtgtTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTc                                                                                                                                 <  2:865583/72‑1 (MQ=255)
                                                                                                 gCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTg                                                   >  1:821480/1‑144 (MQ=255)
                                                                                                 gCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTg                                                   <  2:821480/144‑1 (MQ=255)
                                                                                                           tGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtgacggtga                                   <  2:178406/150‑1 (MQ=255)
                                                                                                           tGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtgacggtga                                   >  2:1239823/1‑150 (MQ=255)
                                                                                                            gATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGAt                                  <  1:772780/150‑1 (MQ=255)
                                                                                                                 cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                          <  1:733194/57‑1 (MQ=255)
                                                                                                                 cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                          >  2:733194/1‑57 (MQ=255)
                                                                                                                 cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCt                             <  1:1239823/150‑1 (MQ=255)
                                                                                                                  ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    >  2:994876/1‑126 (MQ=255)
                                                                                                                  ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  1:994876/126‑1 (MQ=255)
                                                                                                                  ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTg                            >  2:102734/1‑150 (MQ=255)
                                                                                                                           ccGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    >  1:850931/1‑117 (MQ=255)
                                                                                                                           ccGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  2:850931/117‑1 (MQ=255)
                                                                                                                             gATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCa                                                                                                   <  1:291238/68‑1 (MQ=255)
                                                                                                                             gATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCa                                                                                                   >  2:291238/1‑68 (MQ=255)
                                                                                                                                  aGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCTATTTCCGAc            <  2:508494/150‑1 (MQ=255)
                                                                                                                                    gTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACCTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAg          >  1:299040/1‑150 (MQ=255)
                                                                                                                                     tCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGc         <  1:102734/150‑1 (MQ=255)
                                                                                                                                           tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGAc      <  2:1191256/147‑1 (MQ=255)
                                                                                                                                           tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGAc      >  1:1191256/1‑147 (MQ=255)
                                                                                                                                           tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTcg   >  2:344028/1‑150 (MQ=255)
                                                                                                                                           agaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtga                                         >  1:1310840/3‑112 (MQ=255)
                                                                                                                                           agaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtga                                         <  2:1310840/110‑1 (MQ=255)
                                                                                                                                            gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    <  2:251950/99‑1 (MQ=255)
                                                                                                                                            gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                    >  1:251950/2‑100 (MQ=255)
                                                                                                                                            gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTcgc  >  1:162716/2‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGC  >  NC_002947/5967795‑5968084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑