Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,967,946 A→C 10.5% V63G (GTC→GGC)  glnK ← NRII(GlnL/NtrB) phosphatase activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,967,9460AC10.5% 153.4 / 10.1 57V63G (GTC→GGC) glnKNRII(GlnL/NtrB) phosphatase activator
Reads supporting (aligned to +/- strand):  ref base A (27/24);  new base C (3/3);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCGTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGCGCACGTCGTC  >  NC_002947/5967802‑5968094
                                                                                                                                                |                                                                                                                                                    
gcgTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCg                                                                                                                                                 >  1:1037056/1‑150 (MQ=255)
  gTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGTCGCCATCGAt                                                                                                                                               <  2:1012536/150‑1 (MQ=255)
  gTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGAt                                                                                                                                               <  2:1037056/150‑1 (MQ=255)
        aTCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCAc                                                                                                                                         >  1:158053/1‑150 (MQ=255)
        aTCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCAc                                                                                                                                         >  2:817643/1‑150 (MQ=255)
            gTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGa                                                                                                                                                      <  2:1063552/133‑1 (MQ=255)
            gTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGa                                                                                                                                                      >  1:1063552/1‑133 (MQ=255)
             tttCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                                    <  2:316217/150‑1 (MQ=255)
                cGCCGGTACGGATGTGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCa                                                                                                                                 <  1:771085/150‑1 (MQ=255)
                         ggATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGa                                                                                                                        <  2:583638/150‑1 (MQ=255)
                                gATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACca                                                                                                                     <  1:445485/146‑1 (MQ=255)
                                gATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACca                                                                                                                     >  2:445485/1‑146 (MQ=255)
                                  tCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATAtt                                                                                                               <  2:1301458/150‑1 (MQ=255)
                                            cAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACcgcg                                                                                                     >  2:377279/1‑150 (MQ=255)
                                                   accacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGc                                                                                              >  1:178406/1‑150 (MQ=255)
                                                    ccacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCt                                                                                             >  2:575857/1‑150 (MQ=255)
                                                     cacGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTc                                                                                            >  2:1172474/1‑150 (MQ=255)
                                                           aaTCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGtgtg                                                                                      <  1:377279/150‑1 (MQ=255)
                                                            aTCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTgg                                                                                     <  1:575857/150‑1 (MQ=255)
                                                                  ccGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCCTCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTc                                                                               >  1:408822/1‑150 (MQ=255)
                                                                     tCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGc                                                                            >  2:772780/1‑150 (MQ=255)
                                                                       aCCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccg                                                                          >  2:590259/1‑150 (MQ=255)
                                                                         cGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccgac                                                                        >  1:785792/1‑150 (MQ=255)
                                                                            tCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGccgaccga                                                                     >  1:508494/1‑150 (MQ=255)
                                                                                  cGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGGGTGGCCCTTCTGCCGACCGAAACCtt                                                               >  1:300815/1‑150 (MQ=255)
                                                                                   ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  1:590259/150‑1 (MQ=255)
                                                                                   ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  1:817643/150‑1 (MQ=255)
                                                                                   ggTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  2:158053/150‑1 (MQ=255)
                                                                                    gtgtTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTc                                                                                                                                           >  1:865583/1‑72 (MQ=255)
                                                                                    gtgtTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTc                                                                                                                                           <  2:865583/72‑1 (MQ=255)
                                                                                          gCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTg                                                             >  1:821480/1‑144 (MQ=255)
                                                                                          gCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTg                                                             <  2:821480/144‑1 (MQ=255)
                                                                                                    tGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtgacggtga                                             >  2:1239823/1‑150 (MQ=255)
                                                                                                    tGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtgacggtga                                             <  2:178406/150‑1 (MQ=255)
                                                                                                     gATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGAt                                            <  1:772780/150‑1 (MQ=255)
                                                                                                          cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                                    <  1:733194/57‑1 (MQ=255)
                                                                                                          cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTgg                                                                                                                                    >  2:733194/1‑57 (MQ=255)
                                                                                                          cTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCt                                       <  1:1239823/150‑1 (MQ=255)
                                                                                                           ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  1:994876/126‑1 (MQ=255)
                                                                                                           ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              >  2:994876/1‑126 (MQ=255)
                                                                                                           ttCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTg                                      >  2:102734/1‑150 (MQ=255)
                                                                                                                    ccGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  2:850931/117‑1 (MQ=255)
                                                                                                                    ccGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              >  1:850931/1‑117 (MQ=255)
                                                                                                                      gATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCa                                                                                                             >  2:291238/1‑68 (MQ=255)
                                                                                                                      gATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCa                                                                                                             <  1:291238/68‑1 (MQ=255)
                                                                                                                           aGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCTATTTCCGAc                      <  2:508494/150‑1 (MQ=255)
                                                                                                                             gTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACCTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAg                    >  1:299040/1‑150 (MQ=255)
                                                                                                                              tCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGc                   <  1:102734/150‑1 (MQ=255)
                                                                                                                                    tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGAc                <  2:1191256/147‑1 (MQ=255)
                                                                                                                                    tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGAc                >  1:1191256/1‑147 (MQ=255)
                                                                                                                                    tCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTcg             >  2:344028/1‑150 (MQ=255)
                                                                                                                                    agaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtga                                                   <  2:1310840/110‑1 (MQ=255)
                                                                                                                                    agaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTcggtga                                                   >  1:1310840/3‑112 (MQ=255)
                                                                                                                                     gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              >  1:251950/2‑100 (MQ=255)
                                                                                                                                     gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCttt                                                              <  2:251950/99‑1 (MQ=255)
                                                                                                                                     gaTCGATGTCGCCATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTcgc            >  1:162716/2‑150 (MQ=255)
                                                                                                                                               gACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGCGCACgtcgtc  >  2:189585/1‑150 (MQ=255)
                                                                                                                                               gACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGCGCACgtcgtc  <  1:344028/150‑1 (MQ=255)
                                                                                                                                               gACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGCGCACgtcgtc  >  2:292401/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCGTCGGTATCGGTTTCGCCGGTACGGATGCGGATCGCCTGCTCCAGATTCACCACGAAAATCTTGCCGTCACCGATCTTGCCGGTGTTGGCTGCCTTGGTGATGGCTTCGATTACCCGATCAAGGTCTTTGTCATCGATGGCGACATCGATCTTCACCTTGGGCAGGAAATCGACCACATATTCAGCACCGCGATACAGCTCGGTGTGGCCCTTCTGCCGACCGAAACCTTTGACTTCGGTGACGGTGATGCCCTGCACGCCGATTTCCGACAGCGACTCGCGCACGTCGTC  >  NC_002947/5967802‑5968094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑