Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,978,158 T→A 14.8% D9V (GAC→GTC)  PP_5240 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,978,1580TA14.8% 134.3 / 15.1 54D9V (GAC→GTC) PP_5240two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base A (4/4);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

CCCCGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGGTCGTCACCT  >  NC_002947/5978010‑5978301
                                                                                                                                                    |                                                                                                                                               
ccccGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTCGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCAtcgtcgtc                                                                                                                                                <  1:1247507/150‑1 (MQ=255)
  ccGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTcga                                                                                                                                              >  1:1154290/1‑150 (MQ=255)
  ccGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTcga                                                                                                                                              >  1:1258378/1‑150 (MQ=255)
  ccGGCATTTTAAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTcga                                                                                                                                              >  2:54442/1‑150 (MQ=255)
   cGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTcgac                                                                                                                                             >  1:960788/1‑150 (MQ=255)
          ttCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGAATCGTCGTCGACGATGcct                                                                                                                                      <  2:668336/150‑3 (MQ=255)
              ggTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGACGACGATGcctcggt                                                                                                                                  <  1:818115/150‑7 (MQ=255)
                tCCAGCAACACACAGCCCGCCTCGCCCGNCAGTGGCGNAGCCAGGAANGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCGGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACGCct                                                                                                                                >  1:472725/1‑150 (MQ=255)
                 ccAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCctc                                                                                                                               >  2:1247333/1‑150 (MQ=255)
                 ccAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCctc                                                                                                                               <  2:1154290/150‑1 (MQ=255)
                    gCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGc                                                                                                                            <  2:387931/150‑1 (MQ=255)
                    gCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGc                                                                                                                            <  2:210391/150‑1 (MQ=255)
                      aaCACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTc                                                                                                                          >  1:1158435/1‑150 (MQ=255)
                           acaGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCAt                                                                                                                        <  1:1255249/147‑1 (MQ=255)
                           acaGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCAt                                                                                                                        >  2:1255249/1‑147 (MQ=255)
                             aGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGACGACGATGcct                                                                                                                                      >  1:610515/1‑129 (MQ=255)
                             aGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGACGACGATGcct                                                                                                                                      <  2:610515/131‑3 (MQ=255)
                              gCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATg                                                                                                                                         <  2:133569/127‑1 (MQ=255)
                              gCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATg                                                                                                                                         >  1:133569/1‑127 (MQ=255)
                                                           ggAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCgaggag                                                                                     >  1:536984/1‑150 (MQ=255)
                                                                gCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTtgt                                                                                <  2:1158435/150‑1 (MQ=255)
                                                                  gTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGc                                                                              <  1:1247333/150‑1 (MQ=255)
                                                                    gCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATgcg                                                                                                                     >  1:160094/1‑109 (MQ=255)
                                                                    gCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATgcg                                                                                                                     <  2:160094/109‑1 (MQ=255)
                                                                     cccGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATg                                                                           <  2:1258378/150‑1 (MQ=255)
                                                                              aaaCGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGGTCCGAGGAGGGTGTGCATGGCCGCAAGc                                                                  >  1:557322/1‑150 (MQ=255)
                                                                                           tcGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCagcgagc                                                     <  2:1350527/150‑1 (MQ=255)
                                                                                           tcGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCagcgagc                                                     >  1:1350527/1‑150 (MQ=255)
                                                                                             aaaCCCGGCCGAGCGCCGCGGGTTGGCCAGGCTTGTGCGCACCGAGGCATCGTCGTCGCCGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCgg                                                   <  2:472725/149‑1 (MQ=255)
                                                                                                     ccgAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCAt                                           <  2:960788/150‑1 (MQ=255)
                                                                                                           gcagcaGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGtt                                     <  1:54442/150‑1 (MQ=255)
                                                                                                             agcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGAc                                                                                                   >  2:1213076/1‑86 (MQ=255)
                                                                                                             agcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGAc                                                                                                   <  1:1213076/86‑1 (MQ=255)
                                                                                                             agcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCgag                                                                                        <  2:1389101/97‑1 (MQ=255)
                                                                                                             agcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCgag                                                                                        >  1:1389101/1‑97 (MQ=255)
                                                                                                             agcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCa                                   >  2:834574/1‑150 (MQ=255)
                                                                                                              gcagGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCat                                  <  2:992953/150‑1 (MQ=255)
                                                                                                                      gCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCga                                                                                         <  1:603799/87‑1 (MQ=255)
                                                                                                                      gCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCga                                                                                         >  2:603799/1‑87 (MQ=255)
                                                                                                                       ccAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGttctt                         >  1:931278/1‑150 (MQ=255)
                                                                                                                        cAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGttcttc                        >  1:1009625/1‑150 (MQ=255)
                                                                                                                         aGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCCGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTtcttct                       >  2:899209/1‑150 (MQ=255)
                                                                                                                         aGGCTTTTGCGCACCGAGGCATCGGCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCATGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTtcttct                       >  2:1157297/1‑150 (MQ=255)
                                                                                                                          ggCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTcttctt                      <  2:903581/150‑1 (MQ=255)
                                                                                                                           gCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCagcagc                                                         >  1:925794/1‑114 (MQ=255)
                                                                                                                           gCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCagcagc                                                         <  2:925794/114‑1 (MQ=255)
                                                                                                                                   gcACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCtt             >  1:830791/1‑150 (MQ=255)
                                                                                                                                     gggtgtgCATCGTCGACGACGATGCCCACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGt                                      >  1:1240023/7‑123 (MQ=255)
                                                                                                                                     gggtgtgCATCGTCGACGACGATGCCCACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGt                                      <  2:1240023/117‑1 (MQ=255)
                                                                                                                                       cGAGGCATCGTCGTCGACGATGCACACTCCGCTGCTCGTGCGCCGCTCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGCGCATGGCCGCAAGCTCCAGCAGCGAGTGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGgtc         <  1:1289725/150‑1 (MQ=255)
                                                                                                                                       cGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGgtc         <  1:515849/150‑1 (MQ=255)
                                                                                                                                         aGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGgtcgt       <  1:583978/150‑1 (MQ=255)
                                                                                                                                            cATCGTCGACGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATc                                          <  2:70520/112‑1 (MQ=255)
                                                                                                                                            cATCGTCGACGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATc                                          >  1:70520/1‑112 (MQ=255)
                                                                                                                                             aTCGTCGACGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGGTCGTCAcc   >  1:335556/1‑150 (MQ=255)
                                                                                                                                              tcgtcgtcgACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCAt                                           <  1:533727/109‑1 (MQ=255)
                                                                                                                                              tcgtcgtcgACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCAt                                           >  2:533727/1‑109 (MQ=255)
                                                                                                                                              tcgtcgtcgACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGGTCGTCACCt  >  1:1013493/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCCCGGCATTTTCAGGTCCAGCAACACACAGCCCGCCTCGCCCGCCAGTGGCGAAGCCAGGAATGCGTGCCCGGCGGAAAACGACAAGGTCTCGAACCCCGCCGAGCGCAGCAGGTTGGCCAGGCTTTTGCGCACCGAGGCATCGTCGTCGACGATGCACACCCCTCTGCTCATGCGCCGCCCAGGCTCGCAGACCGCGCTCCGAGGAGGTTGTGCATGGCCGCAAGCTCCAGCAGCGAGCGGGACTGCATCTTGTTCATGATGTTCTTCTTGTGCACCTTGGTCGTCACCT  >  NC_002947/5978010‑5978301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑