| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 537,582 | C→G | 12.2% | intergenic (+109/‑44) | PP_mr07 → / → rpoB | P26 ncRNA/DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 537,582 | 0 | C | G | 12.2% | 114.4 / 6.0 | 42 | intergenic (+109/‑44) | PP_mr07/rpoB | P26 ncRNA/DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base G (2/3); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGATTCGTAT > NC_002947/537434‑537730 | ttGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccgcc > 2:1000439/1‑150 (MQ=255) gAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg < 2:62344/150‑1 (MQ=255) gAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg < 1:674834/146‑1 (MQ=255) gAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg > 2:674834/1‑146 (MQ=255) aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGc < 2:376905/150‑1 (MQ=255) ggCTGATGGCTGGTGGCTTATGCCCCCCGACGTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGcc > 1:37378/1‑150 (MQ=255) gCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTTCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGTCa < 1:1420578/150‑1 (MQ=255) cTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCa < 1:757667/150‑1 (MQ=255) tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg < 1:204153/150‑1 (MQ=255) tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGACTGCTCCCGCCGGGAGAAGCCAAACAAGCAg < 2:875473/150‑1 (MQ=255) gtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa > 2:753714/1‑135 (MQ=255) gtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa < 1:753714/135‑1 (MQ=255) cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg < 1:934555/150‑1 (MQ=255) cACCGGCCTGTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg < 1:1000439/150‑1 (MQ=255) ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGgagca < 1:51744/122‑3 (MQ=255) ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGgagca > 2:51744/1‑120 (MQ=255) cTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGc > 1:908363/1‑150 (MQ=255) cTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCCCAAACCCAGGGGCTTGCACGATTTTCTTGCTGCTCCTGTGGGGTGAGTCCAAACATGCAGGTGACCAAGCTGGGGAACGc < 2:520738/150‑1 (MQ=255) gTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAg < 1:761839/131‑1 (MQ=255) gTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAg > 2:761839/1‑131 (MQ=255) ccGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATacac > 2:69639/1‑150 (MQ=255) cGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCCACGGTATCGCCGTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaaaaaa < 2:37378/150‑1 (MQ=255) cTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACt < 1:69639/150‑1 (MQ=255) tGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGc < 1:908769/94‑1 (MQ=255) tGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGc > 2:908769/1‑94 (MQ=255) gccacCCACATGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCa > 2:1161134/3‑150 (MQ=255) acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCaa < 1:272410/148‑1 (MQ=255) acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCaa > 2:272410/1‑148 (MQ=255) acCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGt > 1:1181842/1‑150 (MQ=255) tgtgGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAATTATTTTAGCAAGTTGCCg < 2:1195280/150‑1 (MQ=255) gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGcc < 2:994435/139‑1 (MQ=255) gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGcc > 1:994435/1‑139 (MQ=255) ctccAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGAtg < 1:866619/148‑1 (MQ=255) aCCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGAtgt < 1:894858/150‑1 (MQ=255) ccAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGGCATGGAtgtg > 1:130012/1‑150 (MQ=255) cAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCACAAGGACTTTAGCAAGTTGCCGGACGGCATGTATTTGc > 2:662499/1‑150 (MQ=255) ggTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTAc < 1:662499/150‑1 (MQ=255) gTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTAcc > 1:70658/1‑150 (MQ=255) catgATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg < 2:130012/147‑1 (MQ=255) gcttctCCCGGCGGGAGCAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCaa > 1:911643/1‑92 (MQ=255) gcttctCCCGGCGGGAGCAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCaa < 2:911643/92‑1 (MQ=255) tgctCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGa > 1:594318/1‑150 (MQ=255) tgctCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGa > 1:1381285/1‑150 (MQ=255) ccgccgGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATgg > 2:1392198/1‑114 (MQ=255) ccgccgGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATgg < 1:1392198/114‑1 (MQ=255) gccgGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGATTCGTAt > 2:301381/1‑150 (MQ=255) | TTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTGGATTCGTAT > NC_002947/537434‑537730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |