Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,011,545 T→G 6.6% I114S (ATC→AGC)  rep → single‑stranded DNA dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,011,5450TG 6.6% 171.1 / 4.5 61I114S (ATC→AGC) repsingle‑stranded DNA dependent ATPase
Reads supporting (aligned to +/- strand):  ref base T (27/30);  new base G (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

GGAGCGGGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACCG  >  NC_002947/6011399‑6011694
                                                                                                                                                  |                                                                                                                                                     
ggAGCGGGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCaa                                                                                                                                                    <  2:779962/150‑1 (MQ=255)
 gAGCGGGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAg                                                                                                                                                   <  2:62851/150‑1 (MQ=255)
       gTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCGTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTg                                                                                                                                             <  2:196188/150‑1 (MQ=255)
              ccTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCgg                                                                                                                                      <  2:986231/150‑1 (MQ=255)
                    tgcGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGAcatca                                                                                                                                <  1:981381/150‑1 (MQ=255)
                        cccGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCa                                                                                                                            >  1:357993/1‑150 (MQ=255)
                            ggggAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAg                                                                                                                        <  2:357993/150‑1 (MQ=255)
                                 aGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATa                                                                                                                   <  1:216581/150‑1 (MQ=255)
                                   gCCGTGGCCTGAACGTGTGTACGTTCCACCAGCGGGGCGTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACt                                                                                                                 <  2:157034/150‑1 (MQ=255)
                                    ccGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATTCTAGCCAACGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTc                                                                                                                <  2:749776/150‑1 (MQ=255)
                                              aCCGTGTGTACGTTCCACAACCTAGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTTCGACGTCAAGGCGCTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCgacgac                                                                                                      <  2:630581/150‑1 (MQ=255)
                                                     gtACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcg                                                                                               >  2:417414/1‑150 (MQ=255)
                                                      tACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGtt                                                                                                                                              <  2:601213/102‑1 (MQ=255)
                                                      tACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGtt                                                                                                                                              >  1:601213/1‑102 (MQ=255)
                                                         gTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTc                                                                                                                                        >  1:230820/1‑105 (MQ=255)
                                                         gTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTc                                                                                                                                        <  2:230820/105‑1 (MQ=255)
                                                           tCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACgaga                                                                                         <  1:417414/150‑1 (MQ=255)
                                                            ccACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTc                                                                                                                <  1:563604/126‑1 (MQ=255)
                                                            ccACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTc                                                                                                                >  2:563604/1‑126 (MQ=255)
                                                            ccACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGAt                                                                                        >  1:1138624/1‑150 (MQ=255)
                                                              acaACCTGGGCCTGAACATCATCCGCCAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGCCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCa                                                                                      >  2:189850/1‑150 (MQ=255)
                                                                aaCCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCg                                                                                                                                       >  1:593409/1‑99 (MQ=39)
                                                                aaCCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCg                                                                                                                                       <  2:593409/99‑1 (MQ=38)
                                                                  ccTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGaa                                                                                  >  2:828930/1‑150 (MQ=255)
                                                                    ggggCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACa                                                                                >  2:1422805/2‑150 (MQ=255)
                                                                     gggCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGtt                                                                                                                                              >  1:843326/1‑87 (MQ=255)
                                                                     gggCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGtt                                                                                                                                              <  2:843326/87‑1 (MQ=255)
                                                                          tGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCTATCTTCGACTAGTCCGACATCAAGGCGTCGCTGACGAACATCAGGTATAAGGAAGGCGCCGGCGACGACGTCATCGACGAGACCAAGAACATGATCg                                                                          <  2:49838/150‑1 (MQ=255)
                                                                            aaCCTCTTCCGCACGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATTTTCGACGAGTCTGACATCAATTCGTTGCTTTTGGACATCATTCCGAAGGAATACTCCGGCGACGACAGCATCGAAGAGATCAAGACCATGATCGGt                                                                        >  2:634393/1‑150 (MQ=255)
                                                                              catcatCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGgcgc                                                                      <  1:1422805/150‑1 (MQ=255)
                                                                                  atcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgac                                                                                             <  2:1061282/123‑1 (MQ=255)
                                                                                  atcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgac                                                                                             >  1:1061282/1‑123 (MQ=255)
                                                                                   tcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                              <  1:29386/89‑1 (MQ=255)
                                                                                   tcCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                              >  2:29386/1‑89 (MQ=255)
                                                                                    ccGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaa                                                                <  2:252184/150‑1 (MQ=255)
                                                                                      gCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACgaga                                                                                         <  2:788270/123‑1 (MQ=255)
                                                                                      gCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACgaga                                                                                         >  1:788270/1‑123 (MQ=255)
                                                                                       cAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATc                                                                           >  2:1319542/1‑136 (MQ=255)
                                                                                       cAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATc                                                                           <  1:1319542/136‑1 (MQ=255)
                                                                                          ggAACACGAGCGCCTGGGCTATAAGACGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGACCAAGAACATGATCGGTGCCTGGGCGAACgg                                                          >  1:336949/1‑149 (MQ=255)
                                                                                              cacGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTg                                                      <  1:634393/150‑1 (MQ=255)
                                                                                                 gAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCAGACATCATGCAGAAg                                                                                                                        <  2:517997/81‑1 (MQ=255)
                                                                                                 gAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCAGACATCATGCAGAAg                                                                                                                        >  1:517997/1‑81 (MQ=255)
                                                                                                   gcgcCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCt                                                 <  2:23387/150‑1 (MQ=255)
                                                                                                     gcCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACAGGAACGGCGCCTGGAAGGGCGACCTGGGCCGgc                                               >  1:628468/1‑150 (MQ=255)
                                                                                                      ccTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTAGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCACGAACATGATCGGGGACTGGAAGAACGACCTAGTCCTgcc                                              >  2:617544/1‑150 (MQ=255)
                                                                                                       cTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGACGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGCACATGCTCGGCGCCTGGAAGCACGAGGTGGTCCTgccg                                             >  1:927597/1‑150 (MQ=255)
                                                                                                       cTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTgccg                                             >  2:56543/1‑150 (MQ=255)
                                                                                                               taAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGc                                     >  1:975017/1‑150 (MQ=255)
                                                                                                                 aGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGccc                                   <  1:56543/150‑1 (MQ=255)
                                                                                                                  gCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGGCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCt                                  >  2:423972/1‑150 (MQ=255)
                                                                                                                  gCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTAGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGccca                                  <  2:975017/150‑2 (MQ=255)
                                                                                                                   ccGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGaa                                                                                                                     <  2:101755/66‑1 (MQ=255)
                                                                                                                   ccGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGaa                                                                                                                     >  1:101755/1‑66 (MQ=255)
                                                                                                                             cGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGccc                                   >  2:1401899/1‑138 (MQ=255)
                                                                                                                             cGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGccc                                   <  1:1401899/138‑1 (MQ=255)
                                                                                                                                  ttCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAAc                  >  2:990820/1‑150 (MQ=255)
                                                                                                                                       gatgTCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAgg                          >  2:631656/4‑137 (MQ=255)
                                                                                                                                       gatgTCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAgg                          <  1:631656/134‑1 (MQ=255)
                                                                                                                                        gaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGGACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCcgcgc            >  2:1019378/1‑150 (MQ=255)
                                                                                                                                             cGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCa       <  2:1138624/150‑1 (MQ=255)
                                                                                                                                                  tCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGGGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAccg  >  2:1277797/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGAGCGGGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGACCG  >  NC_002947/6011399‑6011694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑