| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,067,956 | C→G | 6.5% | A181A (GCC→GCG) | PP_5322 → | metal ion transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,067,956 | 0 | C | G | 6.5% | 194.2 / 3.5 | 61 | A181A (GCC→GCG) | PP_5322 | metal ion transporter |
| Reads supporting (aligned to +/- strand): ref base C (24/33); new base G (2/2); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTGGAAATGGGCGAGCTGG > NC_002947/6067808‑6068098 | tCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCg < 1:577257/150‑1 (MQ=255) gCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCGGGcc > 1:1051841/1‑150 (MQ=255) tGGCCCCCAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCg > 2:1055243/1‑150 (MQ=255) cccccAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACACGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGc < 1:1010460/150‑1 (MQ=255) cccccAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCCGTCCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGc < 1:246919/150‑1 (MQ=255) ccccAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGGCTATCTTTGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGcc > 1:449083/1‑150 (MQ=255) ccccAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCTAGGGCGAGcc > 1:605673/1‑150 (MQ=255) cccAAGTCCTGGGCTATCCGCAAGCCAGAGCCGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCt < 1:701493/150‑1 (MQ=255) aaGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGc < 2:292184/150‑1 (MQ=255) acttccagctcgcccatttccacggccgaggccagcacgcgcatgccttggtcgcttgggtcctggccacggctggagtgcaggatcagcttgagttcttcgcggctgtagtggtgctcatggTGCCGGCCAGGGCGAGCCTGGCCCGCa > 2:310260‑M1/124‑150 (MQ=255) tGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCAc > 1:452646/1‑150 (MQ=255) tATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGa > 2:879749/1‑150 (MQ=255) ccGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGca < 2:1362599/150‑1 (MQ=255) gCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGcacc > 1:1213083/1‑150 (MQ=255) aaGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGcaccac > 1:86531/1‑150 (MQ=255) aaGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGcaccac < 1:840208/150‑1 (MQ=255) gCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTa > 1:1172479/1‑150 (MQ=255) cAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACa < 2:1172479/150‑1 (MQ=255) cAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACa < 2:364528/150‑1 (MQ=255) cAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACa > 2:1277066/1‑150 (MQ=255) ctgctgTCGCTGTGCTCCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCcgc < 2:792291/150‑1 (MQ=255) gcggTCGCTGTGGCCCGCCGTGCGGCTTTACCTGTTCTCCTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCga < 1:1127714/147‑1 (MQ=255) gccgaggccagcacgcgcatgccttggtcgcttgggtcctggccacggctggagtgcaggatcagcttgagttcttcgcggctgtagtggtgctcatggTGCGGGCCAGGGCGAGCCTGGccc < 1:1292359‑M1/24‑1 (MQ=255) gccgaggccagcacgcgcatgccttggtcgcttgggtcctggccacggctggagtgcaggatcagcttgagttcttcgcggctgtagtggtgctcatggTGCGGGCCAGGGCGAGCCTGGccc > 2:1292359‑M1/100‑123 (MQ=255) ctgTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCgaa < 2:1213083/150‑1 (MQ=255) ctgTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCgaa < 1:651687/150‑1 (MQ=255) cGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAAc < 1:1214672/150‑1 (MQ=255) gCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCgaa < 1:1080197/145‑1 (MQ=255) gCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCgaa > 2:1080197/1‑145 (MQ=255) cTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTc < 1:608386/150‑1 (MQ=255) gtgGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCaa < 2:1275642/150‑1 (MQ=255) ccgccgTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGa < 1:572259/150‑1 (MQ=255) tttACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCa < 2:865084/150‑1 (MQ=255) ttACCTGTTCTACTGGGCAATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAg < 2:1410226/150‑1 (MQ=255) cTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTCGCACGCTCTATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGt < 2:125779/150‑1 (MQ=255) tGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAg > 2:1347027/1‑147 (MQ=255) tGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAg < 1:1347027/147‑1 (MQ=255) ggCGATGTACCCGGCCATCTACCTGCTCAATGCCCGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGAccc > 1:110110/1‑150 (MQ=255) cGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCTAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCaa < 2:605673/150‑1 (MQ=255) tGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGcac > 2:640667/1‑89 (MQ=255) tGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGcac < 1:640667/89‑1 (MQ=255) gtgcaggatcagcttgagttcttcgcggctgtagtggtgctcatggTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGa < 2:1188849‑M1/33‑1 (MQ=255) gtgcaggatcagcttgagttcttcgcggctgtagtggtgctcatggTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGa > 1:1188849‑M1/47‑79 (MQ=255) ggCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTg > 1:259681/1‑65 (MQ=255) ggCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTg < 2:259681/65‑1 (MQ=255) gCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGc < 2:86531/150‑1 (MQ=255) aCCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGcgcg > 2:1123803/1‑150 (MQ=255) aCCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGcgcg > 1:283347/1‑150 (MQ=255) ccTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGt < 1:1277066/150‑1 (MQ=255) cTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCa < 1:1336997/122‑1 (MQ=255) cTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCa > 2:1336997/1‑122 (MQ=255) ccAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCgg > 1:911328/1‑150 (MQ=255) ccAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCgg > 1:1051307/1‑150 (MQ=255) cAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCa > 1:866859/1‑101 (MQ=255) cAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCa < 2:866859/101‑1 (MQ=255) cAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCACCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGc > 1:1128577/1‑150 (MQ=255) aGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGcc > 2:1001195/1‑150 (MQ=255) ccAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTgg < 1:1055243/150‑1 (MQ=255) tgtagtggtgctcatggTGCGGGCCAGGCTCGCCCTGGCCGGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTGGaa < 1:1117194‑M1/133‑1 (MQ=255) cacCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTGGAAAt < 2:110110/150‑1 (MQ=255) gcATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTGGAAATGGGCGAGCTgg < 2:452646/150‑1 (MQ=255) | TCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAAGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCCAACACCATCCTGCGCATTGCCGGCCAGGGCGAGCCTGGCCCGCACCATGAGCACCACTACAGCCGCGAAGAACTCAAGCTGATCCTGCACTCCAGCCGTGGCCAGGACCCAAGCGACCAAGGCATGCGCGTGCTGGCCTCGGCCGTGGAAATGGGCGAGCTGG > NC_002947/6067808‑6068098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |