| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,089,270 | T→C | 8.3% | Y131Y (TAT→TAC) | potF‑IV → | putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,089,270 | 0 | T | C | 8.3% | 132.7 / 2.9 | 48 | Y131Y (TAT→TAC) | potF‑IV | putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/19); new base C (2/2); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
TCCAACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGC > NC_002947/6089124‑6089418 | tCCAACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATg > 1:343446/1‑150 (MQ=255) ccAACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGt < 2:763108/150‑1 (MQ=255) cacGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTg > 1:1342534/3‑150 (MQ=255) gCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTg > 1:776707/1‑150 (MQ=255) aTCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTc < 2:776707/150‑1 (MQ=255) gtgtTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAg > 2:1032237/1‑150 (MQ=255) cAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTg > 2:1400626/1‑120 (MQ=255) cAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTg < 1:1400626/120‑1 (MQ=255) tCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCgg < 2:1051367/150‑1 (MQ=255) cGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAANCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGATGATCGGGTTCAACCCGGCGAAGGTAAAGGCTGAACTCggg > 1:567338/1‑149 (MQ=255) cagcaAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGa > 2:1164927/1‑150 (MQ=255) agcaAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGAc > 2:1256919/1‑150 (MQ=255) agcaAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGAc > 1:1184219/1‑150 (MQ=255) caAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACaa > 1:1136599/1‑150 (MQ=255) gCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCCCTCGGCTACAACGCGCCGGTGGACAg > 2:1396502/1‑150 (MQ=255) gCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAg > 1:1276980/1‑150 (MQ=255) aaCACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAAc < 1:787409/134‑1 (MQ=255) aaCACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAAc > 2:787409/1‑134 (MQ=255) aaCACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCt < 2:343446/150‑1 (MQ=255) cTGTACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGAc < 1:566987/150‑1 (MQ=255) aCCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGa < 2:1342534/150‑1 (MQ=255) cGGCACTGATGAAGCTGATCGAAGTTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTg < 1:1205846/77‑1 (MQ=255) cGGCACTGATGAAGCTGATCGAAGTTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTg > 2:1205846/1‑77 (MQ=255) aCTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGtata > 1:382018/1‑56 (MQ=255) aCTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGtata < 2:382018/56‑1 (MQ=255) cTGATCAATCTGGTCGACGCTAACGGCCCTGGCAACAAGTTTGCCTTCCCTTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAg < 2:567338/150‑1 (MQ=38) gatgaAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGa > 2:679893/1‑150 (MQ=255) aGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAgaga < 1:679893/150‑1 (MQ=255) gCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGc > 1:1250805/1‑103 (MQ=255) gCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGc < 2:1250805/103‑1 (MQ=255) gCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGaa < 1:1036365/150‑1 (MQ=255) tCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGAc < 1:104989/116‑1 (MQ=255) tCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGAc > 2:104989/1‑116 (MQ=255) gAAGCTAACGACCCGGGCAATCAGTTTGCCGTGCCGTATAGGTACGGCACCGTGCTGATCGGCCGCAACCGGGGCCAGGTCCAGGCTGCACCTGGCACCAACGAGCCGGGGGACAGCTGGAAACTGAGAGTTAACGGAGAGAACATCGcc > 2:796800/1‑150 (MQ=255) gCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTg > 2:237958/1‑85 (MQ=255) gCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTg < 1:237958/85‑1 (MQ=255) gACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCaac > 2:1238329/1‑149 (MQ=255) cccGTGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGACCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCa < 1:796800/150‑1 (MQ=255) ggCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCaa > 2:274014/1‑119 (MQ=255) ggCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCaa < 1:274014/119‑1 (MQ=255) aaacaaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTgcgg > 2:1421944/2‑150 (MQ=255) acaaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGACCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTgcggcg > 1:1212999/1‑150 (MQ=255) aaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCaa < 2:194510/62‑1 (MQ=255) aaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCaa > 1:194510/1‑62 (MQ=255) tttGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGcc < 1:1032237/150‑1 (MQ=255) ggtgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCaa > 1:641331/2‑105 (MQ=255) ggtgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCaa < 2:641331/104‑1 (MQ=255) tgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCg > 2:1187964/1‑150 (MQ=255) ccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGAc < 1:1187964/150‑1 (MQ=255) gtATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTc > 1:502322/1‑150 (MQ=255) atatGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGc > 2:343237/1‑150 (MQ=255) | TCCAACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGC > NC_002947/6089124‑6089418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |