Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 556,085 G→C 6.8% D7H (GAC→CAC)  rplX → 50S ribosomal protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947556,0850GC 6.8% 177.1 / 4.2 59D7H (GAC→CAC) rplX50S ribosomal protein L24
Reads supporting (aligned to +/- strand):  ref base G (26/29);  new base C (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

TGTTCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAACCCGATGGCGGGCGTACAGGGCG  >  NC_002947/555938‑556232
                                                                                                                                                    |                                                                                                                                                   
tgTTCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgac                                                                                                                                                   <  2:1410565/150‑1 (MQ=255)
 gTTCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgacg                                                                                                                                                  >  2:88940/1‑150 (MQ=255)
  ttCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgacga                                                                                                                                                 >  1:333567/1‑150 (MQ=255)
   tCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgacgac                                                                                                                                                <  1:1372670/150‑1 (MQ=255)
        tgAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACgag                                                                                                                                             <  1:1245811/148‑1 (MQ=255)
        tgAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACgag                                                                                                                                             >  2:1245811/1‑148 (MQ=255)
        tgAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTAATGCGATATC‑TCTCGCTCGCCCCTGAAGTGCTGTAAGGAGAACTGACATTCAAAAGATTCGGCGTGacgacgctat                                                                                                                                           >  1:247337/1‑146 (MQ=255)
             aacaaGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCg                                                                                                                                      >  1:249568/1‑150 (MQ=255)
             aacaaGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTCAcgacgaatctttt                                                                                                                                       >  1:1267745/1‑142 (MQ=255)
             aacaaGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTCAcgacgaatctttt                                                                                                                                       <  2:1267745/149‑8 (MQ=255)
                  gcaagAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATc                                                                                                                                 >  1:427816/1‑150 (MQ=255)
                    aagAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGc                                                                                                                               >  2:717438/1‑150 (MQ=255)
                            aTCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTGCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAg                                                                                                                       >  2:822051/1‑150 (MQ=255)
                                 cACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTAAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAACGATTCGTCGTGACGACGAGGTCCTCGTCATCGCCAGCAACGACaac                                                                                                                  >  2:244298/1‑149 (MQ=255)
                                  aCTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGCCGAGATCATCGTGATCGCCGGCAAAGACAAAg                                                                                                                 >  2:169087/1‑150 (MQ=255)
                                  aCTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAg                                                                                                                 <  1:1324474/150‑1 (MQ=255)
                                    tCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgacgac                                                                                                                                                <  1:1336116/117‑1 (MQ=255)
                                    tCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTgacgac                                                                                                                                                >  2:1336116/1‑117 (MQ=255)
                                      gCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTaa                                                                                                             <  1:907692/150‑1 (MQ=255)
                                        aTCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAgc                                                                                                           >  1:1004577/1‑150 (MQ=255)
                                             cGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGt                                                                                                      <  2:1004577/150‑1 (MQ=255)
                                                        cccGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGGGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGaa                                                                                           <  2:843714/150‑1 (MQ=255)
                                                        cccGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGaa                                                                                           <  1:717438/150‑1 (MQ=255)
                                                        cccGGGAACTTCATACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCATCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGaa                                                                                           <  2:134307/150‑1 (MQ=255)
                                                         ccGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGCTCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAg                                                                                          <  1:88940/150‑1 (MQ=255)
                                                             gAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGACGCAGGTTCGTCGTGACGACGATATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTc                                                                                      <  1:174248/150‑1 (MQ=255)
                                                               aCTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTg                                                                                    <  2:427816/150‑1 (MQ=255)
                                                                  tCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCt                                                                                 >  2:1004638/1‑150 (MQ=255)
                                                                    gTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGTAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAACGTGCTGAAGGTTCTGGCtga                                                                               <  1:150788/150‑1 (MQ=255)
                                                                        ttagaAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCaaa                                                                                                                        <  2:1374002/103‑1 (MQ=255)
                                                                        tGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCaaa                                                                                                                        >  1:1374002/1‑105 (MQ=255)
                                                                            aaGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTc                                                                       <  1:822051/150‑1 (MQ=255)
                                                                               ttCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTg                                                                                                >  1:1289932/1‑122 (MQ=255)
                                                                               ttCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTg                                                                                                <  2:1289932/122‑1 (MQ=255)
                                                                                    g‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATc                                                               >  1:273635/1‑150 (MQ=255)
                                                                                        gATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCtga                                                                               >  2:630978/1‑131 (MQ=255)
                                                                                        gATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCtga                                                                               <  1:630978/131‑1 (MQ=255)
                                                                                            gTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGta                                                           >  1:1427260/1‑146 (MQ=255)
                                                                                            gTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTg                                                           <  2:1427260/147‑1 (MQ=255)
                                                                                             tctcGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGt                                                       >  1:1293929/1‑150 (MQ=255)
                                                                                                 gctcgcCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAAc                                                   >  1:634413/1‑150 (MQ=255)
                                                                                                     gcCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTgg                                               <  2:273635/150‑1 (MQ=255)
                                                                                                        ccTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGatca                                                                                                                                         <  1:663613/57‑1 (MQ=255)
                                                                                                        ccTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGatca                                                                                                                                         >  2:663613/1‑57 (MQ=255)
                                                                                                        ccTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCa                                            >  1:1326249/1‑150 (MQ=255)
                                                                                                           gAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCTGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGAGGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGc                                         <  2:249568/150‑1 (MQ=255)
                                                                                                           gAAGTGCTGGAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGc                                         <  1:244298/150‑1 (MQ=255)
                                                                                                              gTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGCCAGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTc                                      <  1:824096/150‑1 (MQ=255)
                                                                                                              gTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCAAGACGACGAGATCATCGTGATCGAAGGCACAGACACAGGGAAGCGCGGTAAGGTGCTGAAGGTTCGGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTc                                      <  2:247337/150‑1 (MQ=255)
                                                                                                                      aGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAg                              <  2:1293929/150‑1 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAg                                                                                                                 >  1:759624/1‑66 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAg                                                                                                                 <  2:759624/66‑1 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAgg                                                                                         <  1:372947/90‑1 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAgg                                                                                         >  2:372947/1‑90 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGc                             >  2:168175/1‑150 (MQ=255)
                                                                                                                       ggAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGGCAAGCGTCATACCAAGc                             <  1:368693/150‑1 (MQ=255)
                                                                                                                           aTCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTaa                         >  1:1190417/1‑150 (MQ=255)
                                                                                                                             ccGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAAcc                       <  2:333567/150‑1 (MQ=255)
                                                                                                                                   tGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAACCCGATgg                 >  2:988754/1‑150 (MQ=255)
                                                                                                                                     cAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAACCCGATggcg               >  2:1171916/1‑150 (MQ=255)
                                                                                                                                      aaaaGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCAt                                    <  2:999015/128‑1 (MQ=255)
                                                                                                                                      aaaaGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCAt                                    >  1:999015/1‑128 (MQ=255)
                                                                                                                                           tctcgtcgTCACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCt                                                                                     <  2:1237520/72‑1 (MQ=255)
                                                                                                                                           tctcgtcgTCACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCt                                                                                     >  1:1237520/3‑74 (MQ=255)
                                                                                                                                                  gtGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAACCCGATGGCGGGCGTACAGGGCg  <  1:988754/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGTTCTGCTGAACAACAAGCAAGAGCCGATCGGCACTCGCATCTTCGGGCCAGTGACCCGTGAACTTCGTACTGAGAAGTTCATG‑AAGATCGTCTCGCTCGCCCCTGAAGTGCTGTAAGGAGATCCGACATGCAAAAGATTCGTCGTGACGACGAGATCATCGTGATCGCCGGCAAAGACAAAGGTAAGCGCGGTAAGGTGCTGAAGGTTCTGGCTGATGACCGTCTGGTTATCGGTGGCGTGAACCTGGTCAAGCGTCATACCAAGCCTAACCCGATGGCGGGCGTACAGGGCG  >  NC_002947/555938‑556232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑