| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 562,663 | C→A | 6.7% | T129N (ACT→AAT) | rpsD → | 30S ribosomal protein S4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 562,663 | 0 | C | A | 6.7% | 183.8 / 3.2 | 60 | T129N (ACT→AAT) | rpsD | 30S ribosomal protein S4 |
| Reads supporting (aligned to +/- strand): ref base C (30/25); new base A (2/2); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
GCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGTTGACGTGGATGCTGCTAAAAA > NC_002947/562515‑562810 | gcCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACt < 2:1388506/150‑1 (MQ=255) cTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGt > 1:502055/1‑150 (MQ=255) tCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTa > 2:1198735/1‑150 (MQ=255) aaGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTc < 2:66421/150‑1 (MQ=255) gcgcAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCAt > 1:1099033/1‑150 (MQ=255) gcAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATcc > 1:874980/1‑150 (MQ=255) gcAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCGCGCCTCGTTCCGAGTCACGTCCGCTTGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATcc < 1:619119/150‑1 (MQ=255) aaCCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCa < 2:509184/145‑1 (MQ=255) aaCCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCa > 1:509184/1‑145 (MQ=255) gTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAg > 1:1152808/1‑150 (MQ=255) tGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGt > 2:144689/1‑150 (MQ=255) cAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCAt > 1:471641/1‑127 (MQ=255) cAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCAt < 2:471641/127‑1 (MQ=255) gCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGt < 2:1099033/150‑1 (MQ=255) cTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTc > 1:736767/1‑150 (MQ=255) cTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTc < 1:267677/150‑1 (MQ=255) tCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCg > 1:591960/1‑150 (MQ=255) tGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTc < 2:502055/150‑1 (MQ=255) ggATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTCAGATTCGAAACATTCCATCCTACCAACTTCGTCCGGGTGACGTGGTCGCAGTCCGCGCGaa > 1:15261/1‑150 (MQ=255) ggATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGaa > 2:670255/1‑150 (MQ=255) gATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGAATGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGaaa > 2:524183/1‑150 (MQ=255) gATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGAATGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGaaa > 2:524254/1‑150 (MQ=255) gtcgtTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTg > 1:1225953/1‑150 (MQ=255) tttACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGcc > 2:1304417/1‑150 (MQ=255) tttACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGcc > 2:999946/1‑150 (MQ=255) tATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCa < 1:484433/88‑1 (MQ=255) tATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCa > 2:484433/1‑88 (MQ=255) gggCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGCCGTGGTCGCAGTCCGCGAGAAATCCCTCGGCTAGGGGCGCAt > 2:397065/1‑150 (MQ=255) cTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGt > 1:1120456/1‑150 (MQ=255) cGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCcgc > 1:826798/1‑118 (MQ=255) cGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCcgc < 2:826798/118‑1 (MQ=255) tCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGcc < 2:1225953/150‑1 (MQ=255) gACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGt > 1:1044524/1‑102 (MQ=255) gACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGt < 2:1044524/102‑1 (MQ=255) aCTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAACGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCtt > 1:889965/1‑150 (MQ=255) aCTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAg > 2:911600/1‑106 (MQ=255) aCTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAg < 1:911600/106‑1 (MQ=255) aCTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCtt < 1:144689/150‑1 (MQ=255) tCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCcgc > 2:935065/1‑109 (MQ=255) tCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCcgc < 1:935065/109‑1 (MQ=255) cGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGaa < 1:1231201/150‑1 (MQ=255) ttCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGccc < 1:564633/143‑1 (MQ=255) ttCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGccc > 2:564633/1‑143 (MQ=255) tCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGCTGCGCATTGTTCAAGCCCTTGAACTGTGCGcc < 1:327822/150‑1 (MQ=255) ccGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGccc < 1:294488/134‑1 (MQ=255) ccGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGccc > 2:294488/1‑134 (MQ=255) ccGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGAATGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCa < 1:524183/150‑1 (MQ=255) ccGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGAATGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCa < 1:524254/150‑1 (MQ=255) cGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTTAACTGTGCGCCCAg > 1:648484/1‑150 (MQ=255) tCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCg > 2:797225/1‑150 (MQ=255) aGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTg < 1:670255/150‑1 (MQ=255) gCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGa < 2:591960/150‑1 (MQ=255) cacaAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAg > 1:967063/1‑150 (MQ=255) acaAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGaa > 2:529586/1‑122 (MQ=255) acaAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGaa < 1:529586/122‑1 (MQ=255) acaAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGt < 2:736767/150‑1 (MQ=255) gCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGtt < 2:1152808/150‑1 (MQ=255) gCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGTTGACGTgg < 2:967063/150‑1 (MQ=255) aaCGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGTTGACGTGGAtgct > 2:1003563/1‑150 (MQ=255) gACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGTTGACGTGGATGCTGCTaaaaa > 2:499413/1‑150 (MQ=255) | GCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGACTCGTTCCGAGTCCCGTCAGCTGGTTTCGCACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAACTGTGCGCCCAGCGTGGCCGCGTTGAGTGGGTTGACGTGGATGCTGCTAAAAA > NC_002947/562515‑562810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |