Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	584,844	A→T	6.7%	L139M (TTG→ATG)	ansA ←	type 1 L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	584,844	0	A	T	6.7%	227.1 / 3.7	71	L139M (TTG→ATG)	ansA	type 1 L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base A (33/33);  new base T (2/3);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTCAGCGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTACCGTG  >  NC_002947/584701‑584985                                                                                                                                                |                                                                                                                                              ccGCTCAGCGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGcgcc                                                                                                                                         <  2:60557/150‑1 (MQ=255)      cAGCGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGcaca                                                                                                                                    <  1:790260/150‑1 (MQ=255)      cAGCGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGcaca                                                                                                                                    <  2:232622/150‑1 (MQ=255)         cGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGt                                                                                                                                 >  1:781990/1‑150 (MQ=255)          gTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGtt                                                                                                                                <  2:922722/150‑1 (MQ=255)                gtggcgtggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCa                                                                                                                          <  2:781990/150‑1 (MQ=255)                gtggcgtggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCa                                                                                                                          <  1:1006122/150‑1 (MQ=255)                     gtggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCAt                                                                                                                     >  2:732212/1‑150 (MQ=255)                     gtggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCAt                                                                                                                     >  1:339814/1‑150 (MQ=255)                     gtggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCAt                                                                                                                     >  2:429475/1‑150 (MQ=255)                     gtggcAGCGCAGCAAAGGCATCGAACGCTTCGCAGCGTAGCTTTGACGCCCGACAGCCGTGCAGCAGCTTCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCAt                                                                                                                     >  1:811393/1‑150 (MQ=255)                      tggcAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATc                                                                                                                    <  1:429475/150‑1 (MQ=255)                               agcaAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCgg                                                                                                           >  1:389500/1‑150 (MQ=255)                               agcaAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCgg                                                                                                           <  2:339814/150‑1 (MQ=255)                                 caAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGa                                                                                                         <  2:1320159/150‑1 (MQ=255)                                 caAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGa                                                                                                         <  2:1025815/150‑1 (MQ=255)                                    aGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGacca                                                                                                      <  2:595635/150‑3 (MQ=255)                                    aGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGacac                                                                                                      <  2:247927/150‑1 (MQ=255)                                    aGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGacac                                                                                                      >  1:1135355/1‑150 (MQ=255)                                     ggCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCAACTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACAcc                                                                                                     >  1:677928/1‑150 (MQ=255)                                      gCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCt                                                                                                    >  1:1288840/1‑150 (MQ=255)                                        aTCGAACGCTTCGCTGCGTAGCTTCGACGCCCCACGATCGTGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGGGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGc                                                                                                  >  1:1182800/1‑150 (MQ=255)                                         tCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGcc                                                                                                 >  1:807782/1‑150 (MQ=255)                                             aCGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGgca                                                                                             >  2:703620/1‑150 (MQ=255)                                              cGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGATGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGgcag                                                                                            >  1:462590/1‑150 (MQ=255)                                                 ttCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCAt                                                                                         <  1:1323699/150‑1 (MQ=255)                                                     cTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAg                                                                                     <  1:874897/150‑1 (MQ=255)                                                      tGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGc                                                                                    >  2:1330605/1‑150 (MQ=255)                                                      tGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGc                                                                                    >  1:1185358/1‑150 (MQ=255)                                                       gCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCt                                                                                                    <  1:614416/133‑1 (MQ=255)                                                       gCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCt                                                                                                    >  2:614416/1‑133 (MQ=255)                                                                      ccGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTgg                                                                    >  1:862043/1‑150 (MQ=255)                                                                        gACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTg                                                                  >  2:769125/1‑150 (MQ=255)                                                                          cAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCt                                                                <  1:1092919/150‑1 (MQ=255)                                                                           aGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTg                                                               <  1:1330605/150‑1 (MQ=255)                                                                            gCCATGCACCAGCTGACCGCATAAGTACAGCTTCACGCCATCTTCCAGGCCGTGTTCGCACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTgg                                                              <  1:783775/150‑1 (MQ=255)                                                                                tGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTg                                                                     <  1:1222820/139‑1 (MQ=255)                                                                                tGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTg                                                                     >  2:1222820/1‑139 (MQ=255)                                                                                   agcagcTGCCCGTGGAAGTACAGCTGCACGCCAACTGCCAGGCCGTGTTCGAACTATCGCCATGCGCCGCACAGGATTTCCCAGGCATCGCTGTCCGGGGCAACCCCCCGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTcc                                                       >  1:1046394/1‑150 (MQ=255)                                                                                     cagcTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAg                                                     <  1:404545/150‑1 (MQ=255)                                                                                       gcTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCa                                                   >  1:873852/1‑150 (MQ=255)                                                                                          gCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACa                                                <  1:720832/150‑1 (MQ=255)                                                                                             cGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGa                                             >  2:526554/1‑150 (MQ=255)                                                                                             cGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTACCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGa                                             >  1:511621/1‑150 (MQ=255)                                                                                              gTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGaa                                            <  2:1288840/150‑1 (MQ=255)                                                                                              gTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGaa                                            >  2:858455/1‑150 (MQ=255)                                                                                               tGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAAc                                           <  2:1030162/150‑1 (MQ=255)                                                                                               tGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAAc                                           <  2:807782/150‑1 (MQ=255)                                                                                               tGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAACATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAAc                                           <  1:725406/150‑1 (MQ=255)                                                                                                           tGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCATTTTTTCTCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACgcagca                               <  2:1182800/150‑1 (MQ=255)                                                                                                              aCGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAgcg                            <  2:462590/150‑1 (MQ=255)                                                                                                                  cATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTg                                                               >  2:752083/1‑111 (MQ=255)                                                                                                                  cATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTg                                                               <  1:752083/111‑1 (MQ=255)                                                                                                                  cATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACg                                    >  1:676706/1‑138 (MQ=255)                                                                                                                  cATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACg                                    <  2:676706/138‑1 (MQ=255)                                                                                                                    tCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAg                      <  2:862043/150‑1 (MQ=255)                                                                                                                      ttCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAg                                                                                     >  2:1378338/1‑85 (MQ=255)                                                                                                                      ttCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAg                                                                                     <  1:1378338/85‑1 (MQ=255)                                                                                                                      ttCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGc                    >  1:1114528/1‑150 (MQ=255)                                                                                                                            gCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCt              >  2:919928/1‑150 (MQ=255)                                                                                                                                tgtTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCg          <  1:919928/150‑1 (MQ=255)                                                                                                                                tgtTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCg          <  1:703620/150‑1 (MQ=255)                                                                                                                                  ttCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGt        >  1:156607/1‑150 (MQ=255)                                                                                                                                  ttCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGt        >  2:1129397/1‑150 (MQ=255)                                                                                                                                    cGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTAc      >  1:195521/1‑150 (MQ=255)                                                                                                                                     gAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTAcc     >  2:675969/1‑150 (MQ=255)                                                                                                                                      gtgcggCGCATTGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACa                                                <  2:1037053/102‑1 (MQ=255)                                                                                                                                      gtgcggCGCATTGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACa                                                >  1:1037053/5‑106 (MQ=255)                                                                                                                                       tgcggCGCATTGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTACCGt   >  2:690390/4‑150 (MQ=255)                                                                                                                                        gcggCGCATTGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTACCGTg  <  1:690390/148‑1 (MQ=255)                                                                                                                                        cTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTACCGTg  <  1:39903/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                              CCGCTCAGCGTCACGGTGGCGTGGCAGCGCAGCAAAGGCATCGAACGCTTCGCTGCGTAGCTTCGACGCCCGACAGCCATGCAGCAGCTGCCCGTGGAAGTACAGCTGCACGCCATCTTCCAGGCCGTGTTCGAACTGGCGCAATGCGCCGCACAGGTTGTCCCAGGCATCGCTGTCCGGGACACCTGCCGGCAGCATCGAGCCGGTCAGCACTACTGGTGCTGGCAGGTCCAGCAACAGGAACGACAACGCAGCAGCGCTGTAGGCCATGCTGTCGGTACCGTG  >  NC_002947/584701‑584985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑