Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 596,127 G→C 8.5% S10R (AGC→AGG)  PP_0506 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947596,1270GC 8.5% 167.5 / 4.7 56S10R (AGC→AGG) PP_0506ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base C (2/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGGCGGCGCGGCA  >  NC_002947/595983‑596275
                                                                                                                                                |                                                                                                                                                    
aCCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGCGCAAAGGCAAAGGCGGTCATAAACGCAGTGAATCGGCGGTTGGCGAGGCAgga                                                                                                                                                 >  2:945266/1‑149 (MQ=255)
 ccGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGcc                                                                                                                                                >  2:1352520/1‑150 (MQ=255)
   gACTATCAAGTCTGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaa                                                                                                                                              <  1:218745/150‑1 (MQ=255)
    aCTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaag                                                                                                                                             <  2:1196858/150‑1 (MQ=255)
     ccaTCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaacc                                                                                                                                            <  1:1092260/148‑3 (MQ=255)
     cTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaagg                                                                                                                                            <  2:467622/150‑1 (MQ=255)
        tCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaaggcaa                                                                                                                                         <  2:546253/150‑1 (MQ=255)
          aGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGCCACACCGACAGGGCAATGGCAAAGGCACTTCGACAAACAGGGAATCGGCGGGTGGCCAGGCTGGCcaaggcaagg                                                                                                                                       >  1:318708/1‑150 (MQ=255)
                   ccGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAgg                                                                                                                              <  2:924989/150‑1 (MQ=255)
                   ccGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAgg                                                                                                                              <  1:399981/150‑1 (MQ=255)
                    cGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAGTGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGt                                                                                                                             >  1:89499/1‑150 (MQ=255)
                        gATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTAAGCACCCGCTACACAGCGAGCAGGAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAACCGCAGTGACTCGGAGGTTGGCCCCGATGGCCCAACTAAGGCGGTGCAGGTACAt                                                                                                                         <  2:1020897/150‑1 (MQ=255)
                         aTGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTgg                                                                                                                                                  >  2:1329622/1‑124 (MQ=255)
                         aTGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTgg                                                                                                                                                  <  1:1329622/124‑1 (MQ=255)
                         aTGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATc                                                                                                                        <  1:43297/150‑1 (MQ=255)
                          tGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCa                                                                                                                       <  2:1405984/150‑1 (MQ=255)
                             tGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGc                                                                                                                    <  2:783170/150‑1 (MQ=255)
                                        cTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCt                                                                                                         >  1:376054/1‑150 (MQ=255)
                                             ccGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAg                                                                                                                               <  1:1363/122‑1 (MQ=255)
                                             aCGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAg                                                                                                                               >  2:1363/1‑123 (MQ=255)
                                               gggCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaac                                                                                                                                             <  1:1290255/107‑2 (MQ=255)
                                               gggCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaac                                                                                                                                             >  2:1290255/1‑106 (MQ=255)
                                                   ttCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCgg                                                                                              <  2:537583/150‑1 (MQ=255)
                                                   ttCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCgg                                                                                              <  1:420652/150‑1 (MQ=255)
                                                           ggACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCa                                                                                          <  1:1309271/146‑1 (MQ=255)
                                                           ggACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCa                                                                                          >  2:1309271/1‑146 (MQ=255)
                                                           ggACCCGCTCAACAGCCAGAAGCAGACACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTAAATCGGAGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAAGTACATAAGGACTCCCGGGGCTTGGCGGCGCGGTTCAGttt                                                                                      >  2:530776/1‑149 (MQ=255)
                                                             aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaac                                                                                                                                             <  1:435109/93‑2 (MQ=255)
                                                             aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaac                                                                                                                                             >  2:435109/1‑92 (MQ=255)
                                                                 gCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAg                                                                                >  1:1330244/1‑150 (MQ=255)
                                                                   tCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCg                                                                              >  1:195598/1‑150 (MQ=255)
                                                                        agccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAgg                                                                         >  1:163310/1‑150 (MQ=255)
                                                                          ccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTg                                                                       <  2:1348849/150‑1 (MQ=255)
                                                                           cagcagcagGCACACCGACAGGGCAATGGCAAAGGCGGTGAGAAACGCAGGGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGGGCGGTTCAGTTCGGCAAGCGACAGGTgg                                                                      >  2:284216/1‑150 (MQ=255)
                                                                           cagcagcagGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTTATGCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTgg                                                                      >  1:1171030/1‑150 (MQ=255)
                                                                              cagcagGCACACCGAGAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAATGCAAGGCGGAGCAGGTATATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCgg                                                                   <  1:123843/150‑1 (MQ=255)
                                                                                     cacaCCGACAGGGCAATGGCAAAGGCGGTCAGAAGCGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGtt                                                                                       <  1:1206434/123‑1 (MQ=255)
                                                                                     cacaCCGACAGGGCAATGGCAAAGGCGGTCAGAAGCGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGtt                                                                                       >  2:1206434/1‑123 (MQ=255)
                                                                                       cacCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgc                                                          <  1:1352520/150‑1 (MQ=255)
                                                                                         ccGACAGGGCAATGTCAAAGGCGGTCAGAAACGCAGGGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGAGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgcgg                                                        <  2:412608/150‑1 (MQ=255)
                                                                                         ccGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgcgg                                                        >  1:287174/1‑150 (MQ=255)
                                                                                          cGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgcggc                                                       <  2:1330244/150‑1 (MQ=255)
                                                                                           gACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGACGTTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgcggcg                                                      >  2:760971/1‑150 (MQ=255)
                                                                                             cAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGcc                                                    <  2:848588/150‑1 (MQ=255)
                                                                                                       gCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACGGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGt                                          >  1:429176/1‑150 (MQ=255)
                                                                                                           aGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTg                                      <  1:945266/150‑1 (MQ=255)
                                                                                                                  cAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCgggg                                                                                                           >  2:1336811/1‑74 (MQ=255)
                                                                                                                  cAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCgggg                                                                                                           <  1:1336811/74‑1 (MQ=255)
                                                                                                                   aGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTg                                      >  2:227547/1‑142 (MQ=255)
                                                                                                                   aGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTg                                      <  1:227547/142‑1 (MQ=255)
                                                                                                                           aGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGATCGAACAGCGGCGCCAGGCTGTGGTCGTGGCTGACGAACAGCAgg                      >  1:683280/1‑150 (MQ=255)
                                                                                                                            gTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgc                                                          >  2:1284224/1‑113 (MQ=255)
                                                                                                                            gTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAgc                                                          <  1:1284224/113‑1 (MQ=255)
                                                                                                                                  cggcggTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCAc               <  2:391595/150‑1 (MQ=255)
                                                                                                                                            ccAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGgcggcgcg     >  1:1158876/1‑150 (MQ=255)
                                                                                                                                             cAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGgcggcgcgg    >  1:731180/1‑150 (MQ=255)
                                                                                                                                               ggCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGGCGGCGCGGCa  >  2:256486/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGGCGGCGCGGCA  >  NC_002947/595983‑596275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑