Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 651,427 T→A 7.0% K414* (AAG→TAG)  dsbD‑I ← thiol/disulfide interchange protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947651,4270TA 7.0% 168.0 / 3.4 57K414* (AAG→TAG) dsbD‑Ithiol/disulfide interchange protein 1
Reads supporting (aligned to +/- strand):  ref base T (21/32);  new base A (2/2);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

TGCCGGCAGGCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGCGTTTTTCACCGTGT  >  NC_002947/651278‑651576
                                                                                                                                                       |                                                                                                                                                     
tgcCGGCAGGCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCtt                                                                                                                                                       >  2:411371/1‑150 (MQ=255)
  ccGGCAGGCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCGGTTGCGCCAGGCGTTGGGCCGTCGTCTTga                                                                                                                                                     >  2:686096/1‑150 (MQ=255)
  ccGGCAGGCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTga                                                                                                                                                     >  1:236567/1‑150 (MQ=255)
       tggCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCAAgacgacn                                                                                                                                                <  2:770407/149‑2 (MQ=255)
         gCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCAAGacgacggc                                                                                                                                              <  2:584900/150‑3 (MQ=255)
         gCNGCCGCTGGCGGCCAGTGACGCCCCNGGCAGTGGGCGCNGTGGGTNGNCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCAAGacgacgga                                                                                                                                              >  1:770407/1‑148 (MQ=255)
                cTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAgc                                                                                                                                       <  1:504318/150‑1 (MQ=255)
                     ggcCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCAcc                                                                                                                                  >  2:56483/1‑150 (MQ=255)
                       ccAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGa                                                                                                                                <  2:253466/150‑1 (MQ=255)
                        cAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAg                                                                                                                               <  1:1175817/150‑1 (MQ=255)
                        cAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAg                                                                                                                               >  1:922653/1‑150 (MQ=255)
                             aCGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTgacgac                                                                                                                                                 >  2:221165/1‑127 (MQ=255)
                             aCGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTgacgac                                                                                                                                                 <  1:221165/127‑1 (MQ=255)
                             aCGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGACGAGTGCGGGCGCCGTACCAGCAAGCCAGCACATACACCA‑ACACCAGTCACGCTG‑GCTTATGTCCCAGGCGTTGGGCCGTCGTCTTGACGACAAATTGCAGCGCACCGAGGAACa                                                                                                                          >  1:101357/1‑150 (MQ=255)
                               gCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGt                                                                                                                        <  1:258696/150‑1 (MQ=255)
                                 ccccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGc                                                                                                                      <  2:101357/150‑1 (MQ=255)
                                 ccccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGc                                                                                                                      <  1:173537/150‑1 (MQ=255)
                                 ccccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGc                                                                                                                      <  2:521184/150‑1 (MQ=255)
                                 ccccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGc                                                                                                                      >  2:319566/1‑150 (MQ=255)
                                  cccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGcc                                                                                                                     >  1:342564/1‑150 (MQ=255)
                                   ccGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCca                                                                                                                    <  2:912837/150‑1 (MQ=255)
                                         gTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCg                                                                                                              >  2:336178/1‑150 (MQ=255)
                                               gcAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGa                                                                                                        <  1:336178/150‑1 (MQ=255)
                                               gcAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAAGTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGa                                                                                                        <  2:207184/150‑1 (MQ=255)
                                                cAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAg                                                                                                       <  1:56483/150‑1 (MQ=255)
                                                cAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAg                                                                                                       <  2:226242/150‑1 (MQ=255)
                                                cAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAg                                                                                                       <  2:643761/150‑1 (MQ=255)
                                                  gTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCAAGGCGTTGGGCCGTCGTGAAAACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGa                                                                                                     >  1:1156140/1‑150 (MQ=255)
                                                    gggTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGaaa                                                                                                   <  2:1168952/150‑1 (MQ=255)
                                                        cGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGa                                                                                               <  1:411371/150‑1 (MQ=255)
                                                         gCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGAc                                                                                              <  1:978198/150‑1 (MQ=255)
                                                           ccTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGcc                                                                                                                     <  1:194733/125‑1 (MQ=255)
                                                           ccTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGAACGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGcc                                                                                                                     >  2:194733/1‑125 (MQ=255)
                                                             tGGCCAGTGAGGGCGCCGTACCAGCAAGCCACCACCTAAATGACTGG‑AGTTCAG‑CCGTGTGGTTGTGCCAGGCGACAGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACcagc                                                                                          <  1:319566/150‑1 (MQ=255)
                                                                       gggCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCg                                                                                >  2:1081286/1‑150 (MQ=255)
                                                                         gcgcCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCACAAGTACGAAGGATGGCACGGCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCa                                                                              <  1:958695/150‑1 (MQ=255)
                                                                                 ccAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGAcgacga                                                                                                                                               >  2:1000964/1‑77 (MQ=255)
                                                                                 ccAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGAcgacga                                                                                                                                               <  1:1000964/77‑1 (MQ=255)
                                                                                  ggcgaaGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGc                                                                     >  2:1052197/5‑150 (MQ=255)
                                                                                   aGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAg                                                                                                       >  1:831864/1‑115 (MQ=255)
                                                                                   aGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAg                                                                                                       <  2:831864/115‑1 (MQ=255)
                                                                                   aGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAACCCGACCAGCAGCAAGGTCGCAGGGCCCGGCa                                                                    <  2:876312/150‑1 (MQ=255)
                                                                                   aGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCa                                                                    <  1:634532/150‑1 (MQ=255)
                                                                                          cAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAg                                                                                                                                        >  2:1423195/1‑75 (MQ=255)
                                                                                          cAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAg                                                                                                                                        <  1:1423195/75‑1 (MQ=255)
                                                                                               cgTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGa                                                        <  1:1052197/150‑1 (MQ=255)
                                                                                                          cA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGAt                                              <  2:922653/150‑1 (MQ=255)
                                                                                                                ccAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCACCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGccc                                         >  2:284069/1‑149 (MQ=255)
                                                                                                                 cAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGc                                           >  2:587395/1‑147 (MQ=255)
                                                                                                                 cAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGc                                           <  1:587395/147‑1 (MQ=255)
                                                                                                                         ccAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCcagca                                >  2:1288063/1‑150 (MQ=255)
                                                                                                                         ccAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCcagca                                >  1:189964/1‑150 (MQ=255)
                                                                                                                           aGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCcagcagc                              <  1:686096/150‑1 (MQ=255)
                                                                                                                                       gctttggCCGTCGTCTTGACGACGAATGCTGACGCACCGAGGAACAGTGCCACACCGGAAGCAAGGAAATCGACCAGCAGCAAGTGCCCAGGGCCCGGCAGGACCCTGCTGATCAATTCGATGGCCAGCCCCAGCAGCAGTACGCCAATg                  <  2:633774/145‑1 (MQ=255)
                                                                                                                                          tGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGCACGGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGCCCGTACCCGGCCCAGCCAGCCGCCGGCCCGCCCCCTCAGCATCCTGCCAATGGCg               <  2:1156140/150‑1 (MQ=255)
                                                                                                                                            ggCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGCGtt             <  2:1200950/150‑1 (MQ=255)
                                                                                                                                                gtcgtcTTGACGCCGAATTCCAGCGCACCGAGGAACAGTGCCCCACCCGCAGCGAGACAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGGGTTTTTc         >  2:123030/1‑150 (MQ=255)
                                                                                                                                                   gtcTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGCGTTTTTCAcc      >  2:1051152/1‑150 (MQ=255)
                                                                                                                                                       tGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGCGTTTTTCACCgtgt  <  1:1081286/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TGCCGGCAGGCTGCCGCTGGCGGCCAGTGACGCCCCCGGCAGTGGGCGCAGTGGGTCGCCCTGGCCAGTGAGGGCGCCGTACCAGCAAGCCAGCGCGTAGACCA‑GCA‑AGGCCAGACCGACCAGTTGCGCCAGGCGTTGGGCCGTCGTCTTGACGACGAATTCCAGCGCACCGAGGAACAGTGCCACACCCGCAGCGAGGAAACCGACCAGCAGCAAGGTCGCAGGGCCCGGCAGTACACGGCTGAGCAAGCCGATGGCCAGCCCCAGCAGCAGTACGCCAATGGCGTTTTTCACCGTGT  >  NC_002947/651278‑651576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑