| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 664,889 | C→T | 15.4% | A584A (GCC→GCT) | yfhM → | lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 664,889 | 0 | C | T | 15.4% | 113.7 / 12.3 | 51 | A584A (GCC→GCT) | yfhM | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (19/24); new base T (4/4); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCTACCAATTCGGCTCGGTCACCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCTGGTGGCCGA > NC_002947/664741‑665038 | gccaCCAATTCGGCTCCGTCGCCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAACTCCCTTTATACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCa < 1:449422/147‑1 (MQ=255) tACCAATTCGGCTCGGTCACCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATAGCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGc < 2:564133/150‑1 (MQ=255) ggCTCGGTCACCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAg > 1:1121626/1‑150 (MQ=255) cTCGGTCACCTAAACCGATCTGAACCATGACGGGGAACTGGACGAAGTCACCCTTGACCAGGCCGGGAAGGCGCTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCCTGCAGGCTGGCCTGCacggtca < 2:220545/150‑7 (MQ=255) cTCGGTCACCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTc > 2:940841/1‑150 (MQ=255) gtaaaCCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTggccg > 2:794811/3‑150 (MQ=255) gccGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGgccggccg > 2:1209937/2‑150 (MQ=255) tGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCAccc > 1:514516/1‑150 (MQ=255) aCCAGGTCCGGGAATTGGACGACGCCACCCGTCTTCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGATCGGCCGGCCACCCgac < 1:191799/150‑3 (MQ=255) cAGGACCTGTAACTGGACGAAGTCACCCTTTTCCGGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGgcgc < 1:483275/150‑1 (MQ=255) ccTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGa < 2:1426570/150‑1 (MQ=255) aCTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCgg > 2:1273682/1‑116 (MQ=255) aCTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCgg < 1:1273682/116‑1 (MQ=255) gACGAAGTCCCCCTTGCCCCGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATc < 1:265610/150‑1 (MQ=255) aCGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCt < 1:1209937/150‑1 (MQ=255) gAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCgg > 1:801330/1‑109 (MQ=255) gAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCgg < 2:801330/109‑1 (MQ=255) aaGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGc < 1:794811/150‑1 (MQ=255) gTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCg > 2:917138/1‑150 (MQ=255) ccTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGa > 1:296383/1‑150 (MQ=255) cTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAg > 2:123101/1‑150 (MQ=255) aCCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGcc > 2:1412850/1‑150 (MQ=255) cccgaagggCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCgg < 1:566679/143‑1 (MQ=255) ccGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCgg < 2:146623/150‑1 (MQ=255) ccGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCgg < 1:123101/150‑1 (MQ=255) ggtggtCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAgg < 1:917138/150‑1 (MQ=255) gtggtCGATATCGAAACCCGTTGGGCAGCACACCTTTCGCCGCGGGAAGTTACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGc < 2:514516/150‑1 (MQ=255) cGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGtt < 2:579770/150‑1 (MQ=255) gAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCCACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGa > 2:520335/1‑79 (MQ=255) gAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGa < 1:520335/79‑1 (MQ=255) gAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGt < 1:124426/150‑1 (MQ=255) ccGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTgagga > 2:292216/1‑150 (MQ=255) cGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTgaggag > 2:577548/1‑150 (MQ=255) cGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa > 2:332555/1‑149 (MQ=255) cGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa < 1:332555/149‑1 (MQ=255) ccGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACgg < 1:940841/150‑1 (MQ=255) gCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGa > 1:922598/1‑150 (MQ=255) cTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa < 1:881732/127‑1 (MQ=255) cTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa > 2:881732/1‑127 (MQ=255) cTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAg > 1:171222/1‑150 (MQ=255) ctcctGCAGGCTGGCCTGCACGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGa < 2:246641/142‑1 (MQ=255) ctcctGCAGGCTGGCCTGCACGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGa > 1:246641/5‑146 (MQ=255) aCCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTgag > 2:1250950/1‑114 (MQ=255) aCCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTgag < 1:1250950/114‑1 (MQ=255) cctGCAGGCTGGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCt > 1:288356/3‑150 (MQ=255) ctGCAGGCTGGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTc > 1:965694/2‑102 (MQ=255) ctGCAGGCTGGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTc < 2:965694/101‑1 (MQ=255) tGCAGGCTGGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa < 2:1268834/120‑1 (MQ=255) tGCAGGCTGGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATa > 1:1268834/1‑120 (MQ=255) tGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCtgg < 1:577548/150‑1 (MQ=255) cAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCtggtg < 2:1121626/150‑1 (MQ=255) cAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCTGGTGGCCGa > 1:485104/1‑150 (MQ=255) | GCTACCAATTCGGCTCGGTCACCGAAACCGATCTGAACCAGGACCTGGAACTGGACGAAGTCACCCTTGACCAGGCCGGCAAGGCGGTGGTCGATATCGAAAGCCGTTGGGCCGAAGCCCGCTCTCCGCTGCAACTGACCGTGCAGGCCAGCCTGCAGGAGTCGGGTGGCCGGCCGATCACCCGGCGCCTGGAGCAGCCTATCTGGCCGGCTGAGCGCCTGCCGGGCCTGCGAGGCCTGTTCGAAGGTGAGGAGACCGATAGCGACGGGCCCGTGGAATTCGAGTTCCTGGTGGCCGA > NC_002947/664741‑665038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |