| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 691,898 | T→C | 12.0% | E156G (GAA→GGA) | yigZ ← | ribosome‑binding factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 691,898 | 0 | T | C | 12.0% | 128.7 / 8.1 | 50 | E156G (GAA→GGA) | yigZ | ribosome‑binding factor |
| Reads supporting (aligned to +/- strand): ref base T (23/21); new base C (3/3); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
GCCCACAGTTGTTGTGGGCAATGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGCGCCGCCGCCATAGGC > NC_002947/691756‑692046 | gCCCACAGTTGTTGTGGGCAATGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAg < 2:135193/150‑1 (MQ=255) acaGTTGTTGTGGGCAATGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTTCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGAcc > 2:344461/1‑150 (MQ=255) tGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAg < 2:1253906/129‑1 (MQ=255) tGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAg > 1:1253906/1‑129 (MQ=255) aGCGTGCTTCGAGCAAAATACGCCCGCGGCTCACGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACTTCGGCCAGAc > 1:219210/1‑150 (MQ=255) aGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCAcc > 1:234294/1‑150 (MQ=255) aaaaTACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGAc > 1:1266511/1‑113 (MQ=255) aaaaTACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGAc < 2:1266511/113‑1 (MQ=255) aaaaTACGCCCGCGGCTCAGGTCCGAACGTTGATGTTGCAATGGCGCGAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAgc < 2:234294/150‑1 (MQ=255) aaTACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAgcgc < 2:277116/150‑1 (MQ=255) gcgGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCt < 2:248997/150‑1 (MQ=255) cgGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAgg > 2:391488/1‑105 (MQ=255) cgGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAgg < 1:391488/105‑1 (MQ=255) cTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTTAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGaaa < 1:940236/150‑1 (MQ=255) gTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAaactgca > 2:899194/1‑150 (MQ=255) gCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCAc > 2:115240/1‑122 (MQ=255) gCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCAc < 1:115240/122‑1 (MQ=255) aGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAg < 1:201353/150‑1 (MQ=255) tGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa > 2:762920/1‑150 (MQ=255) tGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa > 1:552841/1‑150 (MQ=255) ttGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATg > 1:953345/1‑150 (MQ=255) gcCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGgctgcgctgc > 2:847242/1‑150 (MQ=255) gcCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGgctgcgc > 1:786219/1‑147 (MQ=255) gcCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGgctgcgc < 2:786219/147‑1 (MQ=255) cAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCAc < 1:344461/150‑1 (MQ=255) gggCGTCACCGAGGGCAATCACCAGGTCCACACCAGTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACcagca < 2:1360034/150‑1 (MQ=255) ggCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACcagcag < 1:899194/150‑1 (MQ=255) gTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAACAGGCg > 1:1343061/1‑150 (MQ=255) tCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa < 1:665525/126‑1 (MQ=255) tCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa > 2:665525/1‑126 (MQ=255) tCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATACCTGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGt < 2:1108501/150‑1 (MQ=255) cGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATgg > 1:562982/1‑125 (MQ=255) cGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATgg < 2:562982/125‑1 (MQ=255) cGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTgg < 1:762920/150‑1 (MQ=255) cGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTCCGCTGCACCAGCAGGCGTTTgg > 2:1339361/1‑150 (MQ=255) gAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa < 1:730435/121‑1 (MQ=255) gAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGaaa > 2:730435/1‑121 (MQ=255) acCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACttgtt > 2:1277374/1‑150 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc < 1:809198/68‑1 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc > 2:808474/1‑68 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc > 2:808886/1‑68 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc > 2:809198/1‑68 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc < 1:808474/68‑1 (MQ=255) cATTGGCGGTGAACTGTCCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGcc < 1:808886/68‑1 (MQ=255) tGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGgcgc < 2:734244/150‑1 (MQ=255) ggTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGcgccgcc > 2:181965/1‑150 (MQ=255) ggTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGcgccgcc > 2:181385/1‑150 (MQ=255) gTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGgc > 2:1126816/1‑143 (MQ=255) gTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGgc < 1:1126816/143‑1 (MQ=255) gAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGCgccgccgcc > 2:778163/1‑150 (MQ=255) ttCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGCGCCGCCGCCATAGGc > 2:671838/1‑150 (MQ=255) ttCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGCGCCGCCGCCATAGGc > 2:968339/1‑150 (MQ=255) | GCCCACAGTTGTTGTGGGCAATGTTCAGCGTGCTTCGAGCAAAATACGCCCGCGGCTCAGGTCCGCCAGTTGCTGTTGCAATGGCGCCAGGTGGGCGTCACCGAGGGCAATCACCAGGTCCACACCATTGGCGGTGAACTGTTCATCCAGGACCAGGCCGTCAACCTCGGCCAGACGCAGCTTCACCAGCGCCAGCTCGCTGAAACTGCAACTGCAGCTGAAATGGCTGCGCTGCACCAGCAGGCGTTTGGGCGCCTGTTGCAGGCACTTGTTGGCGCCGCCGCCATAGGC > NC_002947/691756‑692046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |