Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 806,330 T→C 9.1% K335E (AAG→GAG)  PP_0693 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947806,3300TC 9.1% 123.7 / 3.0 44K335E (AAG→GAG) PP_0693hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/19);  new base C (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

TTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCGGACT  >  NC_002947/806181‑806458
                                                                                                                                                     |                                                                                                                                
ttCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCtt                                                                                                                                  <  2:838279/150‑1 (MQ=255)
ttCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCtt                                                                                                                                  <  2:317410/150‑1 (MQ=255)
ttCAAGGTCGCGTACCCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCAAGCTCGTTGTGGGCGTCTATGCCTGCAACACGTTGGTCCTCTGGTACCTGATCCTCTTCGGCCAGCTCAAAGCGTTGCtt                                                                                                                                  <  2:14925/150‑1 (MQ=255)
   cAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAg                                                                                                                               >  2:258082/1‑150 (MQ=255)
    aGGTCGCGTACGAGCACCGCGAGGTCTGCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGc                                                                                                                              >  2:350036/1‑150 (MQ=255)
      gTCGCGTACGCGCTCGTCGAGGTCTTCGTCACTGAACTGCCGTGACTGGTCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTGGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCAc                                                                                                                            <  2:1002235/150‑1 (MQ=255)
                    cgtcgAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTa                                                                                                              <  1:165914/150‑1 (MQ=255)
                     gtcgAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCTAAGCGGTGCTTGAGCACCGACAGCTCGc                                                                                                                 <  1:971519/146‑1 (MQ=255)
                     gtcgAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGc                                                                                                                 >  2:971519/1‑146 (MQ=255)
                                   cACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATAc                                                                                               <  1:1063813/150‑1 (MQ=255)
                                       gAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGt                                                                                           >  2:803928/1‑150 (MQ=255)
                                               aagACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCAc                                                                                   <  2:892056/148‑1 (MQ=255)
                                                        ccAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGgc                                                                          <  1:1179452/150‑1 (MQ=255)
                                                          aGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGgcgc                                                                        >  2:730687/1‑150 (MQ=255)
                                                                 cGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAg                                                                            >  2:645940/1‑139 (MQ=255)
                                                                 cGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAg                                                                            <  1:645940/139‑1 (MQ=255)
                                                                         tcgtcgTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGgc                                                                          <  1:1420910/133‑1 (MQ=255)
                                                                         tcgtcgTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGgc                                                                          >  2:1420910/1‑133 (MQ=255)
                                                                                 ggCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGTAGCAGCTCCTGGGTGCCGCCACg                                                 <  2:163420/150‑1 (MQ=255)
                                                                                     tCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTgg                                             >  2:1175693/1‑150 (MQ=255)
                                                                                                 agcagTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCg                                                                                             <  2:862715/90‑1 (MQ=255)
                                                                                                 agcagTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCg                                                                                             >  1:862715/1‑90 (MQ=255)
                                                                                                  gcagTTGCTGCTCCAGTACCTTGACGTCATCGACCAGGGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGc                                >  1:114720/1‑150 (MQ=255)
                                                                                                      ttGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCCCCGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTc                            <  2:114720/150‑1 (MQ=255)
                                                                                                      ttGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTc                            >  2:641250/1‑150 (MQ=255)
                                                                                                        gctgctCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCg                                                                                             <  1:1015079/83‑1 (MQ=255)
                                                                                                        gctgctCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCg                                                                                             >  2:1015079/1‑83 (MQ=255)
                                                                                                        gctgctCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGATCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCt                          >  2:545134/1‑150 (MQ=255)
                                                                                                          tgctCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTACTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGc                        >  1:1361337/1‑150 (MQ=255)
                                                                                                             tCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAgg                                 >  2:166351/1‑138 (MQ=255)
                                                                                                             tCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAgg                                 <  1:166351/138‑1 (MQ=255)
                                                                                                               cAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATc                                                                     <  2:653550/100‑1 (MQ=255)
                                                                                                               cAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATc                                                                     >  1:653550/1‑100 (MQ=255)
                                                                                                                 gTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCAc                 >  1:624202/1‑150 (MQ=255)
                                                                                                                     cTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTc                                       <  1:425133/124‑1 (MQ=255)
                                                                                                                     cTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTc                                       >  2:425133/1‑124 (MQ=255)
                                                                                                                     cTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTa             >  1:1193020/1‑150 (MQ=255)
                                                                                                                            tCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCg      <  1:258082/150‑1 (MQ=255)
                                                                                                                               tcgcaaaGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCGGAc   <  1:350036/144‑1 (MQ=255)
                                                                                                                               tCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCGGAc   <  2:624202/150‑1 (MQ=255)
                                                                                                                                cGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCGGACt  >  1:314745/1‑150 (MQ=255)
                                                                                                                                           tgtcGGTGCTCAAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGc                        >  2:346671/4‑117 (MQ=255)
                                                                                                                                           tgtcGGTGCTCAAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGc                        <  1:346671/114‑1 (MQ=255)
                                                                                                                                            gtcGGTGCTCAAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCa                                                                    >  2:294101/3‑72 (MQ=255)
                                                                                                                                            gtcGGTGCTCAAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCa                                                                    <  1:294101/70‑1 (MQ=255)
                                                                                                                                                   cTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTggg                                                           <  1:1392895/74‑1 (MQ=255)
                                                                                                                                                   cTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTggg                                                           >  2:1392895/1‑74 (MQ=255)
                                                                                                                                                     |                                                                                                                                
TTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCAGCGCGCCGGCCAGCTCGTCGTGGGCGTCTTTGGCCGCCAGCAGTTGCTGCTCCAGTACCTTGACGTCATCGACCAGCGCAAAGCGGTGCTTGAGCACCGACAGCTCGCCTAGCCAGCGCTGGATACCGGTAATTTCACGCTCCAGGCGCATCAGCTCCTGGGTGCCGCCACGCTGGTCGTTCTGCAGGCGGTCCTGCTCGCCACGGTAGTGCTCGGACT  >  NC_002947/806181‑806458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑