| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 68,603 | C→A | 15.5% | G224W (GGG→TGG) | PP_0058 ← | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 68,603 | 0 | C | A | 15.5% | 115.2 / 12.1 | 45 | G224W (GGG→TGG) | PP_0058 | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (21/17); new base A (5/2); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01 | |||||||||||
TCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTACCGCCG > NC_002947/68454‑68751 | tCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGccc < 2:885957/150‑1 (MQ=255) aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGt < 1:1408042/150‑1 (MQ=255) gCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTg < 2:385973/150‑1 (MQ=255) acacaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGCCAACGGCACGGGGCCACGTGCCATTgcc < 1:1305403/150‑3 (MQ=255) acacaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGCCAACGGCACGGGGCCACGTGCCATTgcc < 1:758234/150‑3 (MQ=255) gTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCCCGTGGCCCCGTGCCGTTGGCGTACAt > 2:1397393/1‑150 (MQ=255) ttATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCGTGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATc < 2:1229725/150‑1 (MQ=255) tCCACAGAAGGTCAATCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGc < 1:128803/150‑1 (MQ=255) acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTGGGCGTACATCGGCTCa > 2:926206/1‑150 (MQ=255) ggTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAtt < 2:768347/150‑1 (MQ=255) ccATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACtt > 2:858440/1‑150 (MQ=255) ccATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACtt > 1:19488/1‑150 (MQ=255) tCCTGAGGTGTTTCAGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTa < 1:1360659/150‑1 (MQ=255) ccTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTCCATCGGCTCACCAATTACCACTTCGATTGTAc > 1:1263414/1‑150 (MQ=255) gAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCgg > 2:848388/1‑150 (MQ=255) gTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCAc > 2:685684/1‑150 (MQ=255) tCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTg < 1:926206/150‑1 (MQ=255) cTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAAc < 2:10382/150‑1 (MQ=255) cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTggg > 2:1003746/1‑150 (MQ=255) ccATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGa < 1:1003746/150‑1 (MQ=255) ggCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACtt < 1:848388/150‑1 (MQ=255) gCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCa < 2:1193401/90‑1 (MQ=255) gCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCa > 1:1193401/1‑90 (MQ=255) cccGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGaa > 1:52030/1‑145 (MQ=255) cccGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGaa < 2:52030/145‑1 (MQ=255) cccGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGc > 1:321661/1‑150 (MQ=255) gggTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGt > 1:1080562/1‑150 (MQ=255) tCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgt > 2:325109/1‑150 (MQ=255) ttCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATc > 2:682666/1‑150 (MQ=255) ttCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGACAGAACTTGCCGGCGTTGTGGGCAATc < 2:19488/150‑1 (MQ=255) cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGgc < 1:682666/150‑1 (MQ=255) aaGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGgca > 1:435582/1‑150 (MQ=255) gTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAAc < 1:862083/95‑1 (MQ=255) gTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAAc > 2:862083/1‑95 (MQ=255) tCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCg > 1:1265253/1‑150 (MQ=255) ggCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc > 2:951391/1‑150 (MQ=255) gCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGcc > 1:4436/1‑150 (MQ=255) ccaacggCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATCACCACTTCGACTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGcc > 1:898446/6‑150 (MQ=255) cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCa > 1:962225/1‑150 (MQ=255) cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCa > 2:848107/1‑150 (MQ=255) aacggCACGGGGCCACGTGCCATTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGACTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAg > 1:169391/4‑150 (MQ=255) acggCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAgg > 2:891117/3‑150 (MQ=255) aTGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCg > 1:346134/1‑102 (MQ=255) aTGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCg < 2:346134/102‑1 (MQ=255) ggCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGc < 1:685684/150‑1 (MQ=255) ggCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAccg > 1:1227206/1‑150 (MQ=255) ccccGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAccgcc > 1:463724/1‑150 (MQ=255) cccGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAccgccg > 2:818026/1‑150 (MQ=255) | TCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTACCGCCG > NC_002947/68454‑68751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |