Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 856,084 T→C 7.7% A10A (GCT→GCC)  pagL‑I → lipid A 3‑O‑deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947856,0840TC 7.7% 185.2 / 4.9 65A10A (GCT→GCC) pagL‑Ilipid A 3‑O‑deacylase
Reads supporting (aligned to +/- strand):  ref base T (33/26);  new base C (2/3);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

AGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGCT  >  NC_002947/855936‑856233
                                                                                                                                                    |                                                                                                                                                     
aGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGgctg                                                                                                                                                      >  2:1077929/1‑150 (MQ=255)
    ttGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                                  <  2:489182/150‑1 (MQ=255)
       cGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                               <  2:766898/150‑3 (MQ=255)
        gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCt                                                                                                                                                <  1:1180031/148‑1 (MQ=255)
        gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCt                                                                                                                                                >  2:1180031/1‑148 (MQ=255)
            tgAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGttt                                                                                                                                          <  1:1371565/150‑1 (MQ=255)
             gAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                         >  1:770763/1‑150 (MQ=255)
              aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                        <  2:770763/150‑1 (MQ=255)
              aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                        <  2:505201/150‑1 (MQ=255)
              aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                        <  2:327106/150‑1 (MQ=255)
              aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                        >  1:934973/1‑150 (MQ=255)
                      gTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAga                                                                                                                                >  1:1124336/1‑149 (MQ=255)
                      gTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGc                                                                                                                                >  1:607774/1‑150 (MQ=255)
                              aGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                        <  2:934973/150‑1 (MQ=255)
                                 cTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGccc                                                                                                                     >  2:1147244/1‑148 (MQ=255)
                                    aaCTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTg                                                                                                                                             <  2:56782/123‑1 (MQ=255)
                                    aaCTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTg                                                                                                                                             >  1:56782/1‑123 (MQ=255)
                                            ttCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGAt                                                                                                          >  2:345709/1‑150 (MQ=255)
                                               cACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCGTCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                               >  2:699164/1‑108 (MQ=255)
                                               cACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCGTCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                               <  1:699164/110‑3 (MQ=255)
                                                 cTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                     <  1:1216976/150‑1 (MQ=255)
                                                  ttCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                              >  1:362039/1‑105 (MQ=255)
                                                  ttCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                              <  2:362039/108‑4 (MQ=255)
                                                       cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTc                                                                                               <  1:345709/150‑1 (MQ=255)
                                                        tttCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                              <  1:964465/150‑1 (MQ=255)
                                                            tACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                          <  2:901529/150‑1 (MQ=255)
                                                              ccGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgggg                                                                                        >  1:176965/1‑150 (MQ=255)
                                                                 cTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAg                                                                                     >  1:184489/1‑150 (MQ=255)
                                                                  tttGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAg                                                                                                                   <  2:137098/119‑1 (MQ=255)
                                                                  tttGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAg                                                                                                                   >  1:137098/1‑119 (MQ=255)
                                                                      ccTGAGATGAGGCGGACAAATAACCGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                              <  1:373172/136‑1 (MQ=255)
                                                                      ccTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                              >  2:373172/1‑136 (MQ=255)
                                                                      ccTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                          >  1:1102618/1‑140 (MQ=255)
                                                                      ccTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                          <  2:1102618/140‑1 (MQ=255)
                                                                       cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGc                                                                               >  2:366192/1‑150 (MQ=255)
                                                                        tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                     <  2:546834/127‑1 (MQ=255)
                                                                        tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                     >  1:546834/1‑127 (MQ=255)
                                                                          agaTGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCCGCACAAGCGGCCGAGGTTTCGTTTTCGTTGGGGCAGACTGGCGac                                                                            >  2:1302030/1‑149 (MQ=255)
                                                                            aTGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                          >  2:195949/1‑150 (MQ=255)
                                                                                   ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATg                                                                   <  2:176965/150‑1 (MQ=255)
                                                                                   ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATg                                                                   >  2:281707/1‑150 (MQ=255)
                                                                                   ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATg                                                                   <  1:1302030/150‑1 (MQ=255)
                                                                                    gACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATgg                                                                  >  1:1153153/1‑150 (MQ=255)
                                                                                    gACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATgg                                                                  >  2:90427/1‑150 (MQ=255)
                                                                                     aCAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACt                                                                                  <  1:1415133/133‑1 (MQ=255)
                                                                                     aCAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACt                                                                                  >  2:1415133/1‑133 (MQ=255)
                                                                                         aTAACAGCATCAGAAAGAAGGATGTCAATTAAGATGAAACGACCCTCCCCCACAGCTTCTGCTGCCTTGGTTTTTGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGATTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTAc                                                             >  1:179844/1‑150 (MQ=255)
                                                                                            aCAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCgg                                                          >  2:868777/1‑150 (MQ=255)
                                                                                                     gaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                           >  2:172736/1‑108 (MQ=255)
                                                                                                     gaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                           <  1:172736/108‑1 (MQ=255)
                                                                                                          gagGATCTTTCTTGTTATGAAACTGGGCGGCTTGGTAGCGGCTGCTGCGTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                            <  2:179844/149‑1 (MQ=255)
                                                                                                          aagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATg                                                                   >  2:492800/1‑127 (MQ=255)
                                                                                                          aagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATg                                                                   <  1:492800/127‑1 (MQ=255)
                                                                                                          aagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                            <  1:450465/150‑1 (MQ=255)
                                                                                                                gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                          <  1:473147/98‑1 (MQ=255)
                                                                                                                gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                          >  2:473147/1‑98 (MQ=255)
                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTGTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGGTATGGCTGCAATCGAATTgg                                     >  1:1354672/1‑150 (MQ=255)
                                                                                                                     ttATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                                    <  1:919548/99‑1 (MQ=255)
                                                                                                                     ttATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                                    >  2:919548/1‑99 (MQ=255)
                                                                                                                            gAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtg                          >  2:840709/1‑150 (MQ=255)
                                                                                                                                cTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                      <  1:1077929/150‑1 (MQ=255)
                                                                                                                                    tCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTGGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGa                  >  1:185593/1‑150 (MQ=255)
                                                                                                                                         ttGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGc             >  2:1210367/1‑150 (MQ=255)
                                                                                                                                            gCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGtt          <  1:840709/150‑1 (MQ=255)
                                                                                                                                            gCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGtt          >  1:755291/1‑150 (MQ=255)
                                                                                                                                             cAGCGGCGGCTTCCTTTGTTTTGGGGCATGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTg         <  2:184489/150‑1 (MQ=255)
                                                                                                                                              aGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTgg        >  2:511484/1‑150 (MQ=255)
                                                                                                                                                  gctgctGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCgg    <  1:195949/150‑1 (MQ=255)
                                                                                                                                                    tgctgcCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGCt  >  1:719523/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGCT  >  NC_002947/855936‑856233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑