| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 863,903 | T→A | 17.2% | intergenic (+17/+19) | hemH → / ← uraA | ferrochelatase/uracil permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 863,903 | 0 | T | A | 17.2% | 135.3 / 19.8 | 54 | intergenic (+17/+19) | hemH/uraA | ferrochelatase/uracil permease |
| Reads supporting (aligned to +/- strand): ref base T (24/20); new base A (4/5); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGCCGATCA > NC_002947/863758‑864052 | gAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCgg < 1:947746/150‑1 (MQ=255) gagaTAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGccc < 2:772554/150‑1 (MQ=255) aTAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCtt < 2:715689/150‑1 (MQ=255) tAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTc < 1:152302/150‑1 (MQ=255) tGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCa < 1:220958/150‑1 (MQ=255) tttGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTcgattca < 1:653841/133‑7 (MQ=255) tttGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTcgattca > 2:653841/1‑127 (MQ=255) tGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGcttctt < 2:862842/150‑1 (MQ=255) gAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGc < 1:668276/150‑1 (MQ=255) aGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGc > 2:1219950/1‑111 (MQ=255) aGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGc < 1:1219950/111‑1 (MQ=255) aGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCtt < 2:1375973/119‑1 (MQ=255) aGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCtt > 1:1375973/1‑119 (MQ=255) ccAGGAGCTGGTGCTGGTGCCGTGCCTGAATTATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCa > 1:644239/1‑120 (MQ=255) ccAGGAGCTGGTGCTGGTGCCGTGCCTGAATTATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCa < 2:644239/120‑1 (MQ=255) cAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTtgtg < 1:691998/137‑1 (MQ=255) cAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTtgtg > 2:691998/1‑137 (MQ=255) cAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCa < 1:317175/143‑1 (MQ=255) cAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCa > 2:317175/1‑143 (MQ=255) tggtgctggtgcCGTGCCTGAATGATCACCCGGTGTGGGTGAGGGTGTTTGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCg > 2:1354708/1‑150 (MQ=255) gggtgctggtgcCGTGCCTGAATGATCCCCGGGAGT‑GGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCgg < 1:517917/149‑1 (MQ=255) ccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTcc > 1:683434/1‑92 (MQ=255) ccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTcc < 2:683434/92‑1 (MQ=255) gtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGt > 2:1164782/1‑128 (MQ=255) gtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGt < 1:1164782/128‑1 (MQ=255) gccTGAATGATCACCCGGAGTGGGTGAGTTTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCa < 1:1161/150‑1 (MQ=255) cTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACAt < 1:1249663/100‑1 (MQ=255) cTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACAt > 2:1249663/1‑100 (MQ=255) gAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGc > 1:304386/1‑150 (MQ=255) gATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATa < 2:304386/150‑1 (MQ=255) aTCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGTGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAg > 2:1194425/1‑150 (MQ=255) tCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTtgtg > 2:135031/1‑105 (MQ=255) tCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTtgtg < 1:135031/105‑1 (MQ=255) cGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACaa > 2:1272152/1‑150 (MQ=255) ggTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATa < 2:936342/136‑1 (MQ=255) ggTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATa > 1:936342/1‑136 (MQ=255) gTGAGGGTGTTGGCCGATATGTGAGAAAAGGCCTGAATCTAAGGGCCCTGAGCACCACCCCGTCCGAGCGATCATACATCCAGCTTCTTGGGCTTCCAGCTGTCATTACCCGGCAGAACCAGGTGCAGGGCAATCCCCACAATTGCGcac > 2:199920/1‑150 (MQ=255) tGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTc > 2:1360231/1‑63 (MQ=25) tGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTc < 1:1360231/63‑1 (MQ=25) tGAGGGTGTTGGCTGATATGTGTGAAAAGGCCGGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGcaca < 2:891611/150‑1 (MQ=255) gATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTc > 2:1068884/1‑150 (MQ=255) tgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGccc > 1:594091/1‑150 (MQ=255) tgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCa > 1:102371/1‑150 (MQ=255) gAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATc < 2:1359740/56‑1 (MQ=255) gAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATc > 1:1359740/1‑56 (MQ=255) gAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTAATCCGGGc < 1:1194425/150‑1 (MQ=255) gAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCa > 2:256140/1‑150 (MQ=255) gAAGGGGCCGCAAAGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCa > 2:477042/1‑150 (MQ=255) aaGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCAc > 2:99819/1‑150 (MQ=255) aaGGGGCCGCTTTGCGGCCCCTTCCCACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATCGCGCACAGGGCGATGCCCCTCAGGCACCAGTCATCCGGGCCGTCAc > 2:308952/1‑150 (MQ=255) gggCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGc > 2:1006510/1‑150 (MQ=255) gCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCCCCGcgg > 1:130624/1‑148 (MQ=255) gCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTg > 2:864346/1‑150 (MQ=255) gCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTg > 2:1345289/1‑150 (MQ=255) tGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGCCGATCa < 2:594091/150‑1 (MQ=255) | GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGCCGATCA > NC_002947/863758‑864052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |