Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 961,583 T→A 7.3% S279C (AGC→TGC)  selD ← selenide, water dikinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947961,5830TA 7.3% 158.4 / 3.9 55S279C (AGC→TGC) selDselenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base A (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

AGGCCCAGTTCGGCAGCCACGGCGAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGG  >  NC_002947/961444‑961725
                                                                                                                                           |                                                                                                                                              
aGGCCCAGTTCGGCAGCCACGGCGAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGGAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTg                                                                                                                                      >  1:15088/1‑150 (MQ=255)
 ggCCCAGTTCGGAAGCACCGGCGCGGACCTCGGCTTCTCCCACCGGTCCCTCCGCTACCCGCTAGCCACCGCTGGTCTTCCGGTCGCATTGCAGGTTCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTgc                                                                                                                                     <  2:1365813/150‑1 (MQ=255)
                 cACGGCGAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGt                                                                                                                                        >  2:595957/1‑131 (MQ=255)
                 cACGGCGAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGt                                                                                                                                        <  1:595957/131‑1 (MQ=255)
                       gAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGGTCCACCGGGGATGcg                                                                                                               >  2:1151595/1‑149 (MQ=255)
                              tCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCg                                                                                                        >  2:492777/1‑150 (MQ=255)
                              acGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCg                                                                                                        >  2:973860/2‑150 (MQ=255)
                                ggCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGc                                                                                                      >  2:243718/1‑150 (MQ=255)
                                   ttCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAg                                                                                                   >  2:1215144/1‑150 (MQ=255)
                                   ttCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAg                                                                                                   >  1:553769/1‑150 (MQ=255)
                                           ccGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGt                                                                                           <  2:516591/150‑1 (MQ=255)
                                            cGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTc                                                                                          >  1:1250601/1‑150 (MQ=255)
                                                 cAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGacac                                                                                     >  2:822775/1‑150 (MQ=255)
                                                 cAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGacac                                                                                     <  1:822775/150‑1 (MQ=255)
                                                  aaCAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCAGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACt                                                                                    >  1:676626/1‑150 (MQ=255)
                                                  aaCAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCAGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACt                                                                                    >  1:677109/1‑150 (MQ=255)
                                                    cAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATgg                                                                                            >  1:614644/1‑140 (MQ=255)
                                                    cAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATgg                                                                                            <  2:614644/140‑1 (MQ=255)
                                                     aGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATTGTCGACACTagg                                                                                 >  2:682873/1‑150 (MQ=255)
                                                       cTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGTGGATGCAGCCTTCGTCCAGGTAATCGTCGACACGacgca                                                                               >  2:289337/1‑150 (MQ=255)
                                                            aGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGt                                                                          <  1:839729/150‑1 (MQ=255)
                                                                    aCCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCAcggc                                                                  >  2:922669/1‑150 (MQ=255)
                                                                      cGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTc                                                                                          <  1:52818/124‑1 (MQ=255)
                                                                      cGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTc                                                                                          >  2:52818/1‑124 (MQ=255)
                                                                          ggTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTa                                                            <  1:1190050/150‑1 (MQ=255)
                                                                                cGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAg                                                      <  1:973860/150‑1 (MQ=255)
                                                                                cGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCACAGGGCGCTGCACCAGTGCCTGTATCTGTCGTAGTTTCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAg                                                      >  1:1316742/1‑150 (MQ=255)
                                                                                   gTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGt                                                                                           >  2:633078/1‑110 (MQ=255)
                                                                                   gTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGt                                                                                           <  1:633078/110‑1 (MQ=255)
                                                                                        acaGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCa                                                                                                               >  2:1349458/1‑85 (MQ=255)
                                                                                        acaGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCa                                                                                                               <  1:1349458/85‑1 (MQ=255)
                                                                                           gccggTGCTTCTGGTCGTCGCTCAGGGCGCTGATCATGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGa                                           <  1:1365842/147‑1 (MQ=255)
                                                                                             agGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAgg                                         >  1:1323352/1‑150 (MQ=255)
                                                                                               gTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGACAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGcc                                       >  2:648560/1‑150 (MQ=255)
                                                                                                   ttCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGc                                     >  2:1331385/1‑148 (MQ=255)
                                                                                                   ttCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGc                                     <  1:1331385/148‑1 (MQ=255)
                                                                                                   ttCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTg                                   >  1:828616/1‑150 (MQ=255)
                                                                                                        gtcgtcGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGt                                             <  1:465925/135‑1 (MQ=255)
                                                                                                        gtcgtcGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGt                                             >  2:465925/1‑135 (MQ=255)
                                                                                                        gtcgtcGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTc                              <  2:1250601/150‑1 (MQ=255)
                                                                                                         tcgtcgCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCg                             <  1:1215144/150‑1 (MQ=255)
                                                                                                         tcgtcgCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCg                             <  2:1006667/150‑1 (MQ=255)
                                                                                                         tcgtcgCTCAGGGCGCTGATCTTGTGCCCGTAGCAGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCg                             <  2:676626/150‑1 (MQ=255)
                                                                                                         tcgtcgCTCAGGGCGCTGATCTTGTGCCCGTAGCAGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCg                             <  2:677109/150‑1 (MQ=255)
                                                                                                            tcgCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAg                                                      >  1:1227090/1‑122 (MQ=255)
                                                                                                            tcgCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAg                                                      <  2:1227090/122‑1 (MQ=255)
                                                                                                               cTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAAGTACTGGTCGCCACTAGGCCGGAGTGGCACGACGGCGTAGTTGATTTGCACGGTGAGGCCGCTGCCTTCGGCCAGc                       <  2:15088/150‑1 (MQ=255)
                                                                                                                     gcgcTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTca                             >  1:473275/1‑137 (MQ=255)
                                                                                                                     gcgcTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTca                             <  2:473275/138‑2 (MQ=255)
                                                                                                                             cTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGcc         <  2:553769/150‑1 (MQ=255)
                                                                                                                                gtgCCCGTAGCTGTCTTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGt                                                                                           <  1:1243433/65‑1 (MQ=255)
                                                                                                                                gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGt                                                                                           >  2:1243433/1‑65 (MQ=255)
                                                                                                                                    ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAgg  <  2:1316742/150‑1 (MQ=255)
                                                                                                                                      gTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGctg   >  1:484085/1‑145 (MQ=255)
                                                                                                                                      gTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTGCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGctg   <  2:484085/147‑3 (MQ=255)
                                                                                                                                           |                                                                                                                                              
AGGCCCAGTTCGGCAGCCACGGCGAGGAACTCGGCTTCACCCTCCGGGGCAACAGCTACCAGCAAGCCACCGCTGGTCTGCGGGTCGCACAGCAGGTGCTTCTGGTCGTCGCTCAGGGCGCTGATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGG  >  NC_002947/961444‑961725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑