Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 966,599 A→T 9.7% L41M (TTG→ATG) ‡ PP_0828 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947966,5990AT 9.7% 119.9 / 4.7 41L41M (TTG→ATG) ‡PP_0828membrane protein
Reads supporting (aligned to +/- strand):  ref base A (14/23);  new base T (2/2);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCG  >  NC_002947/966450‑966740
                                                                                                                                                     |                                                                                                                                             
gCTGCGCGAACTGTTCACCAGCGCGCCCCACAGCGCCGGCGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCCCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCaa                                                                                                                                               <  1:784775/150‑1 (MQ=255)
        aaCTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCa                                                                                                                                       <  2:1208273/150‑1 (MQ=255)
        aaCTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAACAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCa                                                                                                                                       <  1:1002670/150‑1 (MQ=255)
          cTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCTTGAACAGCaag                                                                                                                                     <  2:821395/150‑3 (MQ=255)
             ttCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGaaa                                                                                                                                  <  1:1252993/150‑1 (MQ=255)
               cACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATc                                                                                                                                >  1:948040/1‑150 (MQ=255)
                aCCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCTTGAACAGCaa                                                                                                                                      <  2:565148/143‑2 (MQ=255)
                aCCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCTTGAACAGCaa                                                                                                                                      >  1:565148/1‑142 (MQ=255)
                 ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGACCAGCATGAAATCgc                                                                                                                              <  2:1307796/150‑2 (MQ=255)
                 ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGa                                                                                                                              <  2:44132/150‑1 (MQ=255)
                  cAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGAt                                                                                                                             <  2:136171/150‑1 (MQ=255)
                   aGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATg                                                                                                                            <  1:1115951/150‑1 (MQ=255)
                        gcCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGAAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGAc                                                                                                                       <  1:303293/150‑1 (MQ=255)
                                 ggTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATg                                                                                                                            <  1:813053/136‑1 (MQ=255)
                                 ggTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATg                                                                                                                            >  2:813053/1‑136 (MQ=255)
                                  gTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATcc                                                                                                             <  1:1270144/150‑1 (MQ=255)
                                            aacagcaGCGCCTACAGCCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCaa                                                                                                                                               <  2:1382282/101‑1 (MQ=255)
                                            aacagcaGCGCCTACAGCCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCaa                                                                                                                                               >  1:1382282/6‑106 (MQ=255)
                                              caccaGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCagcagc                                                                                                 >  1:579673/1‑150 (MQ=255)
                                                        tGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCg                                                                                       >  1:921385/1‑150 (MQ=255)
                                                         gTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGa                                                                                      <  2:1381614/150‑1 (MQ=255)
                                                                      ttGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAg                                                                         <  2:948040/150‑1 (MQ=255)
                                                                               cTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTGCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTc                                                                <  2:579673/150‑1 (MQ=255)
                                                                                tCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCg                                                                               >  1:499900/1‑134 (MQ=255)
                                                                                tCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCg                                                                               <  2:499900/134‑1 (MQ=255)
                                                                                  aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTg                                                                                                                         <  2:901272/90‑1 (MQ=255)
                                                                                  aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTg                                                                                                                         >  1:901272/1‑90 (MQ=255)
                                                                                  aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTc                                                             >  2:289280/1‑150 (MQ=255)
                                                                                    cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGTATAAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCTATGGTGATCAGCACATCCTAGCTCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTcac                                                           <  2:389861/150‑1 (MQ=255)
                                                                                    cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGAt                                                                    >  2:1211813/1‑141 (MQ=255)
                                                                                    cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGAt                                                                    <  1:1211813/141‑1 (MQ=255)
                                                                                          caccaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGACGGTCTTCACCACCAg                                                     >  2:8896/1‑150 (MQ=255)
                                                                                           accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGaa                                                                                                                                            <  2:610625/62‑1 (MQ=255)
                                                                                           accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGaa                                                                                                                                            >  1:610625/1‑62 (MQ=255)
                                                                                           accaGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAgg                                                    >  1:41361/1‑150 (MQ=255)
                                                                                                 gcTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTAc                                              <  2:928267/150‑1 (MQ=255)
                                                                                                          agcagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCg                                                  >  1:540919/1‑137 (MQ=255)
                                                                                                          agcagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCg                                                  <  2:540919/137‑1 (MQ=255)
                                                                                                          agcagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCg                                     >  1:121865/1‑150 (MQ=255)
                                                                                                            cagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGAt                                   <  2:41361/150‑1 (MQ=255)
                                                                                                            cagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGAt                                   <  1:289280/150‑1 (MQ=255)
                                                                                                                     cGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCgg                                                 <  2:150164/127‑1 (MQ=255)
                                                                                                                     cGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCgg                                                 >  1:150164/1‑127 (MQ=255)
                                                                                                                                            tGCTGTTCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCAGGCTTTGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgc   >  1:88008/1‑150 (MQ=255)
                                                                                                                                                    aaGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg  <  2:1401826/143‑1 (MQ=255)
                                                                                                                                                    aaGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg  >  1:1401826/1‑143 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCG  >  NC_002947/966450‑966740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑