| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 968,878 | G→T | 15.0% | intergenic (‑308/+23) | PP_0831 ← / ← PP_t68 | hypothetical protein/tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 968,878 | 0 | G | T | 15.0% | 96.5 / 5.0 | 37 | intergenic (‑308/+23) | PP_0831/PP_t68 | hypothetical protein/tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base T (3/3); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATC > NC_002947/968739‑969025 | ttCTGAATATAACTGACGAATGAAAGCTTAAGGCCTAGCGCAAGGGGCGGACAATAGAATACAGGTATGGGGGGAGTCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGt < 2:608947/150‑1 (MQ=255) tCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGtt < 2:961248/150‑1 (MQ=255) atatCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCg < 2:1264601/150‑1 (MQ=255) tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt > 1:687196/1‑150 (MQ=255) aTGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGt < 2:327562/131‑1 (MQ=255) aTGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGt > 1:327562/1‑131 (MQ=255) cTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAggg < 2:368006/150‑1 (MQ=255) ggCTAGCGCAAGGGGCGGCCAATAGAAGGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTc < 1:1051974/149‑1 (MQ=255) gaGCGCAAGGGGCGCCAAATAGAAAGGAGTGATGGGGGGAGGCGCGGGAGCCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGaa < 1:1023616/149‑1 (MQ=255) gcggcCAATAGAATGCAGGTATGGGGGGAGGCGCGGGATTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCt < 1:858361/150‑1 (MQ=255) ggcCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACGCCCACTCCttt > 1:1002020/1‑150 (MQ=255) gcCAATAGAATGCAGGTATGGGGGGAGGCGTGGTGGCGCAGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTc < 2:1182921/150‑1 (MQ=255) aaTAGGATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaa > 2:1422247/1‑150 (MQ=255) tAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaaaa > 2:1305018/1‑150 (MQ=255) tGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAt > 1:1094981/1‑150 (MQ=255) tGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAt < 2:1094981/150‑1 (MQ=255) aGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtttt < 2:687196/150‑1 (MQ=255) gTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGGCGGTGTTTTCCGGATTTGGTGCCCCGTGGGAGACTCGAACTCCCACTCCTTTCGAACACGGGTTGTGa > 1:241065/1‑150 (MQ=255) aGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAAt < 2:1384937/150‑1 (MQ=255) ggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCa < 2:158129/150‑1 (MQ=255) aaCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTcc < 1:253431/88‑1 (MQ=255) aaCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTcc > 2:253431/1‑88 (MQ=255) cgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCg > 1:1135481/1‑55 (MQ=255) cgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCg < 2:1135481/55‑1 (MQ=255) tgcgtgAATCGAAGCTACAAAAAAACCGACCTTAAGGTCGGTTTTTTACGCAATTCGTGGCCGTTGGCGGAGTCCAACTCCCCCTCCTTTCGAAAACGGATTTTCAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAg < 1:878844/150‑1 (MQ=255) aGGTACAAAAAGACCGACCGTAGGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCAAACTCCCACTCCTTTCGAAAGTGGATTTTGAATCCGCCGAGTATACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTg < 2:241065/150‑1 (MQ=255) acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc < 2:800363/128‑1 (MQ=255) acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc > 2:828660/1‑150 (MQ=255) acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGACATCAGGGCGTGgc > 1:800363/1‑128 (MQ=255) ggaaaaaaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGTAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTg < 1:890944/123‑1 (MQ=255) ggaaaaaaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTg > 2:890944/3‑125 (MQ=255) caAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCt > 1:346259/1‑150 (MQ=255) aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTc < 1:1056913/67‑1 (MQ=255) aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTc > 2:1056913/1‑67 (MQ=255) aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCa < 1:960616/60‑1 (MQ=37) aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCa > 2:960616/1‑60 (MQ=37) aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg > 1:816397/1‑150 (MQ=255) ccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATc > 1:158303/1‑150 (MQ=255) cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg < 1:1251368/143‑1 (MQ=255) cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg > 2:1251368/1‑143 (MQ=255) | TTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATC > NC_002947/968739‑969025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |