| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 970,240 | T→G | 15.2% | F29C (TTC→TGC) ‡ | tgt → | queuine tRNA‑ribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 970,240 | 0 | T | G | 15.2% | 124.9 / 16.3 | 59 | F29C (TTC→TGC) ‡ | tgt | queuine tRNA‑ribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (25/24); new base G (3/6); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TCATCACCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGC > NC_002947/970094‑970379 | tcatcaCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCac < 2:977279/150‑2 (MQ=255) atcaCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGTATTCATGc > 1:612229/1‑150 (MQ=255) tcaCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGcc > 1:1395600/1‑150 (MQ=255) aCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCgg < 1:450195/150‑1 (MQ=255) aCCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCgg < 1:1362922/150‑1 (MQ=255) cccGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGt < 2:1366934/150‑1 (MQ=255) cccGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCggg < 2:257102/150‑2 (MQ=255) cGCAATGCGGCGACAGGCGGGCCCGAGGATCAAGCCTGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCAACGACGGCAAGGCCCGTCGGGGTCGCCTCACCTTCCCACGTGGCACCGTGGAACCCCCGCCATTCATGCCGGTgg > 2:1361455/1‑150 (MQ=255) cGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTgg < 1:1077538/150‑1 (MQ=255) gCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCggggtt < 1:342437/150‑5 (MQ=255) cGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTa < 1:990893/150‑1 (MQ=255) gcCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACACCGGCATGAATGCCggggt < 1:1226402/139‑4 (MQ=255) gcCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACACCGGCATGAATGCCggggt > 2:1226402/1‑136 (MQ=255) aCGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACg < 1:340910/150‑1 (MQ=255) gCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAg > 2:1243139/1‑150 (MQ=255) gCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGCATGCCGGTGGGGACCTATGGCACGGTCAAg < 2:876929/150‑1 (MQ=255) ccGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAggg > 2:1253420/1‑150 (MQ=255) gAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCa < 1:33304/150‑1 (MQ=255) aaGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGACATGCTGcc > 1:60371/1‑150 (MQ=255) aGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATgg < 2:552447/128‑1 (MQ=255) aGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATgg > 1:552447/1‑128 (MQ=255) aTGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCAcgc < 2:60371/150‑1 (MQ=255) gCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCg > 2:1062273/1‑150 (MQ=255) aCCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAg > 1:466391/1‑150 (MQ=255) aCCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCggg > 1:224236/1‑103 (MQ=255) aCCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATGAATGCCggg < 2:224236/104‑2 (MQ=255) ccTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGCTCCAGCGGAAACCCCCTCAGCCATGCTGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAgg < 1:1243139/150‑1 (MQ=255) gAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCg > 1:412378/1‑150 (MQ=255) aTGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGgc < 1:820912/150‑1 (MQ=255) cTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAg > 2:705445/1‑112 (MQ=255) cTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAg < 1:705445/112‑1 (MQ=255) cTTCGAACTGCTGGCCACCGACGGAACGGGCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCga < 1:360895/150‑1 (MQ=255) gAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAgatg > 2:1066009/1‑150 (MQ=255) aCTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAgatgat > 1:1165772/1‑150 (MQ=255) ctgGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTc < 1:1066009/150‑1 (MQ=255) tgGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCg < 1:146240/150‑1 (MQ=255) ccGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAaca > 2:390001/1‑150 (MQ=255) cGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAt < 1:1370360/99‑1 (MQ=255) cGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCAt > 2:1370360/1‑99 (MQ=255) cGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAacac > 2:653412/1‑150 (MQ=255) aaGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCAc < 2:1395600/150‑1 (MQ=255) gCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCga > 1:31408/1‑122 (MQ=255) gCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCga < 2:31408/122‑1 (MQ=255) ccGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTgggg > 2:1404757/1‑64 (MQ=255) ccGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTgggg < 1:1404757/64‑1 (MQ=255) ccGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATAGAGGCCATCGGCGACGAGATGAACCTCGGCAACACCTTCCACCtgtg > 2:867156/1‑150 (MQ=255) aTCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAacac > 2:991980/1‑125 (MQ=255) aTCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAacac < 1:991980/125‑1 (MQ=255) aTCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCgg > 1:255828/1‑148 (MQ=255) aTCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCgg < 2:255828/148‑1 (MQ=255) tCCCACGGGGCACCGTCCAACCCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATCATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAgg < 2:466391/150‑1 (MQ=255) ccACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATc > 1:1244518/1‑105 (MQ=255) ccACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATc < 2:1244518/105‑1 (MQ=255) aCGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGAt > 1:780379/1‑150 (MQ=255) ccGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAac > 2:394844/1‑104 (MQ=255) ccGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAac < 1:394844/104‑1 (MQ=255) cGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAgg > 1:899606/1‑74 (MQ=255) cGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAgg < 2:899606/74‑1 (MQ=255) ccaccGGCATGAATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGc > 2:1331291/4‑150 (MQ=255) | TCATCACCCGCAATCCGGCGCCACGCGGGCCCGAGGATCAAGCATGAGTCGCACCTGTCGAATGTCCTTCGAACTGCTGGCCACCGACGGCAAGGCCCGTCGGGGTCGCATCACCTTCCCACGTGGCACCGTGGAAACCCCGGCATTCATGCCGGTGGGGACCTATGGCACGGTCAAGGGCATGCTGCCACGCGACATCGAGGCCATCGGCGCCGAGATGATCCTCGGCAACACCTTCCACCTGTGGCTGCGTCCGGGCACCGAGGTGATCAAGAAGCACAACGGC > NC_002947/970094‑970379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |