Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	77,503	A→T	9.1%	V14E (GTG→GAG)	rsmB ←	ribosomal RNA small subunit methyltransferase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	77,503	0	A	T	9.1%	199.9 / 9.1	66	V14E (GTG→GAG)	rsmB	ribosomal RNA small subunit methyltransferase B
Reads supporting (aligned to +/- strand):  ref base A (36/24);  new base T (3/3);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

CAGCAGCTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCAGGCTCAGGGCTT  >  NC_002947/77358‑77652                                                                                                                                                   |                                                                                                                                                      cagcagCTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGc                                                                                                                                                   >  1:373664/1‑150 (MQ=255)  agcagcTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCg                                                                                                                                                  >  2:1206978/1‑150 (MQ=255)  agcagcTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCg                                                                                                                                                  >  2:1027008/1‑150 (MQ=255)      gcTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGTGCTCAGCGGcaa                                                                                                                                              <  1:1309490/150‑3 (MQ=255)          tgCGGCCAGCAGGTCGACGCGTGGCAGACAGCGTGTGGTACGGAACGCGAAGGGCTGGGTGAAGCCGAGGTGGCGTTCATCTTCTTTATCTAGTTGCGCCGGCCGCGAGCTGTTCAGAGAAGCCTT‑GCCGCTGAGCACAGCGGCCAgcgc                                                                                                                                          >  2:521405/1‑150 (MQ=255)              gCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACgg                                                                                                                                      <  1:676620/150‑1 (MQ=255)               ccAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGTGCTCAGCGGCAAg                                                                                                                                             >  2:539662/1‑142 (MQ=255)               ccAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGTGCTCAGCGGCAAg                                                                                                                                             <  1:539662/142‑1 (MQ=255)                  gcagGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGc                                                                                                                                                >  1:1177118/1‑136 (MQ=255)                  gcagGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGc                                                                                                                                                <  2:1177118/136‑1 (MQ=255)                  gcagGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGggcgg                                                                                                                                  >  2:139504/1‑150 (MQ=255)                                ggCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGTCGACGCCACGGTCATCTACCTTGTCCAGTTGAACCTGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTggg                                                                                                                    <  1:1385987/150‑1 (MQ=255)                                   tGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTc                                                                                                                 >  2:150515/1‑150 (MQ=255)                                    cctggCGTTCGGTACCGCACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGTGCTCAGCGGcaa                                                                                                                                              >  1:1405772/5‑118 (MQ=255)                                    cctggCGTTCGGTACCGCACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGTGCTCAGCGGcaa                                                                                                                                              <  2:1405772/116‑3 (MQ=255)                                      cAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCAtt                                                                                                              <  1:1376344/150‑1 (MQ=255)                                      cAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCAtt                                                                                                              >  1:183580/1‑150 (MQ=255)                                      cAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCAtt                                                                                                              >  2:684517/1‑150 (MQ=255)                                       aGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTg                                                                                                             <  2:268208/150‑1 (MQ=255)                                        gCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTgg                                                                                                            <  1:150515/150‑1 (MQ=255)                                              ggTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGc                                                                                                      >  2:778926/1‑150 (MQ=255)                                                tACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGt                                                                                                                       <  2:812542/131‑1 (MQ=255)                                                tACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTACAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGt                                                                                                                       >  1:812542/1‑131 (MQ=255)                                                     aaCGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGc                                                                                               >  2:1077653/1‑150 (MQ=255)                                                      aCGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGcc                                                                                              <  1:213348/150‑1 (MQ=255)                                                           aGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCcggcgg                                                                                         <  1:684517/150‑1 (MQ=255)                                                              tCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGa                                                                                      >  2:390006/1‑150 (MQ=255)                                                               ccTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGaa                                                                                     <  1:584928/150‑1 (MQ=255)                                                                cTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAGGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGTCCCAGCACCTTGCCGGCGGCGAAc                                                                                    >  1:1203069/1‑150 (MQ=255)                                                                   ggTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTtc                                                                                 >  1:171732/1‑150 (MQ=255)                                                                         gCCGCGGTCGCGTTCATCTACCTTTTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGCACTTCTCgcgg                                                                           >  1:57277/1‑150 (MQ=255)                                                                           cgcgGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCtt                                                                                                 <  1:608382/126‑1 (MQ=255)                                                                           cgcgGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCtt                                                                                                 >  2:608382/1‑126 (MQ=255)                                                                            gcgGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGcggcgg                                                                        >  1:815826/1‑150 (MQ=255)                                                                               gTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTg                                                                     >  2:1352984/1‑150 (MQ=255)                                                                                  gcgTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTg                                                                  <  2:171732/150‑1 (MQ=255)                                                                                    gTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAAc                                                                                    >  2:1089327/1‑130 (MQ=255)                                                                                    gTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAAc                                                                                    <  1:1089327/130‑1 (MQ=255)                                                                                     tgtaTCTACCTTGTGCAGTTGCTCCTGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAAc                                                               >  2:1090560/4‑150 (MQ=255)                                                                                         tCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCTTCGGCTGTTAAGCAGGt                                                           >  2:793003/1‑150 (MQ=255)                                                                                         tCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGt                                                           <  2:815826/150‑1 (MQ=255)                                                                                          cTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTc                                                          >  2:82842/1‑150 (MQ=255)                                                                                            aCCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCAc                                                        >  2:537076/1‑150 (MQ=255)                                                                                             ccTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACt                                                       >  1:986301/1‑150 (MQ=255)                                                                                             ccTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACt                                                       <  1:96624/150‑1 (MQ=255)                                                                                               ttGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGa                                                     >  1:1090990/1‑150 (MQ=255)                                                                                                tGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGaa                                                    <  2:1090990/150‑1 (MQ=255)                                                                                                tGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGaa                                                    <  1:139504/150‑1 (MQ=255)                                                                                                 gTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGACGTGTTCGCCG‑GCAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAg                                                   <  1:1027008/150‑1 (MQ=255)                                                                                                       ttGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAAt                                             <  1:1206978/150‑1 (MQ=255)                                                                                                               gCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGACGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTgcc                                     >  1:110703/1‑150 (MQ=255)                                                                                                                   cGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGCACAGGTCACTGAAGCTCAATGCCTTGCCGCCa                                 <  1:741672/150‑1 (MQ=255)                                                                                                                         gTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTgcc                                     >  2:492243/1‑140 (MQ=255)                                                                                                                         gTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTgcc                                     <  1:492243/140‑1 (MQ=255)                                                                                                                          ttCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGtt                          >  1:947976/1‑150 (MQ=255)                                                                                                                          ttCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACCTCTTTCCTGTGCCGGCTAACTGGTGAGGGAAGCTCAATGCCTTGCCGCCAGGCAGtt                          <  1:603991/150‑1 (MQ=255)                                                                                                                            cAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGc                        <  1:476299/150‑1 (MQ=255)                                                                                                                              gCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAg                      >  2:438920/1‑150 (MQ=255)                                                                                                                               cGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAgg                     >  1:504652/1‑150 (MQ=255)                                                                                                                                 aaGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCg                   >  1:136105/1‑150 (MQ=255)                                                                                                                                     cTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTc               >  1:744995/1‑150 (MQ=255)                                                                                                                                      tt‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCa              >  2:901623/1‑150 (MQ=255)                                                                                                                                       tggCCGCTGTGCTCAGCGGCCAGCGCACGGGCGGCGGCGAGGAGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCa              >  2:392049/3‑150 (MQ=255)                                                                                                                                          ccGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAgg                               >  2:1154629/1‑130 (MQ=255)                                                                                                                                          ccGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGCTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAgg                               <  1:1154629/130‑1 (MQ=255)                                                                                                                                           cGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCAGGCt          >  1:893811/1‑150 (MQ=255)                                                                                                                                                   acaGCGGCCAGCGCACGGGCGGCGGCGCGCGGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCAGGCTCAGGGCtt  <  1:1071008/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CAGCAGCTGTGCGGCCAGCAGGTCGAGGCGTGGCTGCCAGCGTGCGGTACCGAACGCCAGGTCCTGGGTCAGGCCGCGGTCGCGTTCATCTACCTTGTCCAGTTGCGCCGGCAGCGAGCTGTTCAGCGAAGCCTT‑GCCGCTGAGCACAGCGGCCAGCGCACGGGCGGCGGCGAGGCGTGGGTTCATTGGCCCAGCACCTTGCCGGCGGCGAACTTCTCGCGGCGGCTGTTGAACAGGTCACTGAAGCTCAATGCCTTGCCGCCAGGCAGTTGCAGGCGGGTCAGGCTCAGGGCTT  >  NC_002947/77358‑77652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑